BioTek: Microplate Instrumentation & Software Solutions
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Eon Microplate Spectrophotometer

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High performance monochromator-based absorbance

Fast, compact automation with BioStack

The Eon™ Microplate Spectrophotometer is the latest in BioTek’s line of high performance, high value microplate instruments. Eon’s optical system allows wavelength selection between 200 and 999 nm in as little as 1 nm increments. No filters required for a new assay - Eon has it covered. It’s easy to view the sample’s peak wavelengths - just run a quick spectral scan in the cuvette, or in a microplate well. Eon reads 6- to 384-well microplate formats, and offers an optional cuvette port. Eon offers BioTek’s innovative 4-Zone™ temperature control system and takes it up a notch to 65°C. The higher setpoint capability expands application possibilities without the need for other instrumentation. Eon comes with Gen5™ v 2.0 and is compatible with Take3™  Micro-Volume plates for 2 µL measurements, as well as BioCell™, and cuvette measurements. Eon is BioStack™ and 3rd party automation compatible.

 Take3 Trio Compatible       Take3 Trio Compatible

  • 6- to 384-well microplate reading
  • 200 to 999 nm wavelength range
  • Optional cuvette port (200 – 999 nm)
  • Orbital, double-orbital and linear shaking
  • Incubation to 65°C
  • BioStack™ and 3rd party automation compatibility
  • Gen5™ v 2.0 Software

6- to 384-well plates. Incubation to 65°C, orbital and linear shaking

6- to 384-well plates. Incubation to 65°C. Cuvette port, orbital and linear shaking

Wavelength Selection Monochromator
Read method Endpoint, kinetic, spectral scanning and well area scanning
Microplate types 6- to 384-well plates, Take3 and Take3 Trio Micro-Volume Plates
Other Labware Standard cuvettes (EonC model)
Temperature control Ambient +4°C to 65°C
Shaking Orbital, double orbital and linear
Software Gen5™ Data Analysis software
Automation BioStack™ and 3rd party automation compatible
Light source Xenon flash lamp
Wavelength range 200 - 999 nm, selectable in 1 nm increments
Bandpass 2.4 nm
Dynamic range 0.0 - 4.0 OD
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy 0 to 2.0 OD + 1% + 0.010
2.0 to 3.0 OD + 3% + 0.010
OD linearity 0 to 2.0 OD + 1% + 0.010
2.0 to 3.0 OD + 3% + 0.010
OD repeatability 0 to 2.0 OD + 1% + 0.005 2.0 to 3.0 OD + 3% + 0.005
Stray light 0.03% at 230 nm typical
Reading speed 96 wells: 8 seconds
384 wells: 13 seconds
Physical Characteristics
Connectivity USB and RS232 ports for PC connection
Power 100 – 240 Volts AC 50/60 Hz
Dimensions 16" D x 8.5" W x 8.5" H
(40.6 cm x 21.6 cm x 21.6 cm)
Weight <24 lbs (11 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change.


Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


BioStack Integration Kits

7310049 BioStack Integration Kit for Eon

Labware Holders

7330531 Holder for 60-/72-/96-well Terasaki Plates accessory has picture


7210515 3-Instrument Rack – for third party robotic system integration accessory has picture

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
GENRC Gen5 Reader Control (RC) accessory has picture

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture
Take3Trio Take3 Trio Micro-Volume Plate accessory has picture

Test Plates & Reagents

7120782 BioTek QC Check Solution #1 - Yellow (125 mL) accessory has picture

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