Cytation Multi-Mode Reader

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Cytation Multi-Mode Reader

Modular and Upgradable: For current and future workflow needs

100 mW Alpha Laser: Speed and performance-based excitation for Alpha assays

100 mW Alpha Laser: Speed and performance-based excitation for Alpha assays

Patented Hybrid OpticsThe Cytation family of multi-mode readers offer modularity to meet every laboratory's present workflow needs, and are upgradable for future requirements. Cytation readers feature the patented Hybrid Technology™ which incorporates variable bandwidth monochromator optics and high-sensitivity filter-based detection optics for unmatched versatility and performance. Variable bandwidth monochromators allow selection of excitation and emission wavelengths in 1 nm increments for top and bottom fluorescence measurements, UV-Vis absorbance and luminescence. The filter module is independent, with its own light source and high performance dichroic-based wavelength selection for advanced detection modes like fluorescence polarization, time-resolved fluorescence and filtered luminescence (e.g. BRET), along with available laser-based excitation for Alpha detection. Cell-based assays are optimized with Cytation's incubation up to 65 °C, orbital and linear shaking, plus available CO2/O2 control and dual reagent injectors. Data collection and analysis are managed with Gen5™ software, specifically designed for uncomplicated processing of even the most complex assays.

  • Patented Hybrid Technology™ combines flexible monochromator detection with high performance dichroic-based filter detection
  • Laser-based excitation available for AlphaScreen® AlphaLISA® assays
  • Available variable bandwidth fluorescence monochromators offer 9 nm to 50 nm in 1 nm increments
  • Optional CO2/O2 control, incubation up to 45 °C or 65 °C and shaking to optimize cell-based assays
  • Low volume (2 μL) nucleic acid and protein quantification with the available Take3™ Plates
  • Gen5™ Microplate Reader and Imager Software reader control, advanced data analysis and flexible output in one easy-to-use software package
  • Modular, upgradable hardware design allows the choice of filter- and monochromator-based multi-mode optics, optional gas control and reagent injectors
  • Available fluorescence microscopy module
Monochromator optics for flexible top and bottom measurements Monochromator optics for flexible top and bottom measurements
Filter optics offer excellent sensitivity  Monochromator optics for flexible top and bottom measurements
   
Variable bandwith monochromators to optimize detection of many fluorophores  
Variable bandwith monochromators to optimize detection of many fluorophores  




“Alpha” refers to products/technologies from PerkinElmer, Inc., that carry trademarks or registered trademarks.




 
Part #
CYT5MFA
CYT5MF
CYT5FA CYT3MF CYT5F CYT3F CYT5M CYT3M
FILTER-based                
Fluorescence intensity
   
Fluorescence polarization
   
Time resolved fluorescence
   
Filtered luminescence
   
Alpha detection
 
 
 
 
   
MONOCHROMATOR-based                
UV-Vis absorbance
 
   
Fluorescence intensity
 
   
Time resolved fluorescence*
 
   
Variable bandwidth fluorescence monochromators
       
 
Luminescence
 
   
OTHER                
Take3 Plate compatible
 
   
Incubation to 65 °C
 
 
 
Incubation to 45 °C      
 
 
Upgradable to imaging

*TRF is a secondary mode in monochromator-only configurations

All configurations are compatible with optional dual reagent injector, CO2/O2 controller and BioStack.

 

General
Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence
Alpha (Cytation 5)
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Filters: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 45 °C (Cytation 3)
4-Zone incubation to 65 °C (Cytation 5) with Condensation Control™
+0.2 °C at 37 °C
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: +0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2; +0.2% at 1% O2
Models for both CO2 and O2 or CO2 only are available
Absorbance
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 230 - 999 nm, 1 nm increments
Monochromator bandwidth 4 nm (230 - 285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Fluorescence Intensity
Light source Xenon flash
Detector PMT for monochromator system
PMT for filter system
Wavelength selection Quad monochromators (top/bottom)
Filters (top)
Wavelength range Monochromators: 250 - 700 nm (850 nm option)
Filters: 200 - 700 nm (850 nm option)
Monochromator bandwidth Variable; from 9 to 50 nm, in 1 nm increments (Cytation 5)
Fixed, 16 nm (Cytation 3)
Dynamic range 5 decades (Cytation 3)
7 decades (Cytation 5)
Sensitivity Filters:
Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)

Quad Monochromator:
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
Luminescence
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity Monos: 20 amol ATP (flash)
Filters: 10 amol ATP (flash), 100 amol (glow)
Fluorescence Polarization
Light source Xenon flash
Detector PMT
Wavelength selection Filters
Wavelength range 280 - 700 nm (850 nm option)
Sensitivity 1.2 mP standard deviation at 1 nM fluorescein
Time-Resolved Fluorescence
Light source Xenon flash
Detector PMT
Wavelength selection Quad monochromators (secondary mode)
Filters (top)
Wavelength range Filters: 200 - 700 nm (850 nm option)
Sensitivity Filters: Europium 40 fM (4 amol/well, 384-well plate)
Monos: Europium 1200 fM (120 amol/well, 384-well plate)
Alpha Detection
Light source 100 mW 680 nm laser
Detector PMT
Wavelength selection Filters (top)
Sensitivity 100 amol LCK peptide (384-well low volume plate)
Reagent Dispensers
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well microplates, Petri dishes
Dead volume 1.1 mL, 100 µL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL
Physical Characteristics
Power 250 Watts max.
Dimensions 20" D x 16.5" W x 17.5" H
(50.8 cm x 41.91 cm x 44.5 cm)
Weight 80 lbs (36.3 Kg)
Regulatory
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Microplate Reader and Imager Software

Gen5 Microplate Reader and Imager Software

BioTek’s Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.
Note: Some instruments come with a specific edition of Gen5, please check the microplate reader specifications.


Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Optional

Adapters and Labware Holders

1222240 Petri dish adapter 38.7 mm max accessory has picture
1222241 Petri dish adapter 60 mm accessory has picture
1222242 Petri dish adapter 100 mm accessory has picture
1222246 Petri dish adapter 35.5 mm max accessory has picture

BioStack Integration Kits

7310053 BioStack Integration Kit for Cytation

Filter Accessories

7082073 Plug (Dummy filter) for Synergys and FLx800 accessory has picture
7082075 Retainer Clip for Synergys and FLx800 accessory has picture

Filter Cubes

1325000 Alpha filter block for 96 well measurements
1325001 Alpha filter cube for 384 well measurements
8040501 Filter Cube: Blue (360/460) / Green (485/528) accessory has picture
8040502 Filter Cube: Green (485/528) / Red (530 / 590) accessory has picture
8040505 Filter Cube: HTRF® – TR-FRET accessory has picture
8040506 Filter Cube: LanthaScreen – TR-FRET accessory has picture
8040507 Filter Cube: Lance – TR-FRET accessory has picture
8040553 Filter Cube – Luminescence accessory has picture
8040555 TRF cube
8040561 Filter Cube: Green FP (485/528) accessory has picture
8040562 Filter Cube: Red FP (530 / 590) accessory has picture
8040563 Blue/UV FP filter set: 360/40 EX, 460/40 EM, 400 nm mirror, UV polarizers
8040564 Empty Filter Cube - Fluorescence Polarization Visible (excitation > 400nm)
8040565 Empty Filter Cube - Fluorescence Polarization UV (excitation between 300 and 400 nm)
8040566 Empty Filter Cube - non FP applications
8040578 Deep UV FP 284/340

Filters

7092146 276/10 nm Fluorescence Filter accessory has picture
7092148 280/10 nm Fluorescence Filter accessory has picture
7082259 284/10 nm Fluorescence Filter accessory has picture
7082248 310/20 nm Fluorescence Filter accessory has picture
7082250 320/20 nm Fluorescence Filter accessory has picture
7092205 325/50 nm Fluorescence Filter accessory has picture
7092150 330/20 nm Fluorescence Filter accessory has picture
7082263 330/80 nm Fluorescence Filter accessory has picture
7082254 340/11 nm Fluorescence Filter accessory has picture
7082230 340/30 nm Fluorescence Filter accessory has picture
7092151 355/20 nm Fluorescence Filter accessory has picture
7082220 360/40 nm Fluorescence Filter accessory has picture
7092152 380/11 nm Fluorescence Filter accessory has picture
7082228 380/20 nm Fluorescence Filter accessory has picture
7092153 395/25 nm Fluorescence Filter accessory has picture
7082242 400/10 nm Fluorescence Filter accessory has picture
7082205 400/30 nm Fluorescence Filter accessory has picture
7092154 405/20 nm Fluorescence Filter accessory has picture
7092155 410/10 nm Fluorescence Filter accessory has picture
7092200 410/80 nm Fluorescence Filter accessory has picture
7082270 420/27 nm Fluorescence Filter accessory has picture
7082206 420/50 nm Fluorescence Filter accessory has picture
7082227 440/30 nm Fluorescence Filter accessory has picture
7082207 440/40 nm Fluorescence Filter accessory has picture
7082208 450/50 nm Fluorescence Filter accessory has picture
7082222 460/40 nm Fluorescence Filter accessory has picture
7092157 470/40 nm Fluorescence Filter accessory has picture
7092158 475/20 nm Fluorescence Filter accessory has picture
7082221 485/20 nm Fluorescence Filter accessory has picture
7082209 485/40 nm Fluorescence Filter accessory has picture
7092160 490/10 nm Fluorescence Filter accessory has picture
7082268 495/10 nm Fluorescence Filter accessory has picture
7092162 500/20 nm Fluorescence Filter accessory has picture
7082256 500/27 nm Fluorescence Filter accessory has picture
7082218 508/20 nm Fluorescence Filter accessory has picture
7092201 515/30 nm Fluorescence Filter accessory has picture
7082246 516/20 nm Fluorescence Filter accessory has picture
7092163 520/10 nm Fluorescence Filter accessory has picture
7092164 520/20 nm Fluorescence Filter accessory has picture
7082247 528/20 nm Fluorescence Filter accessory has picture
7082223 530/25 nm Fluorescence Filter accessory has picture
7092166 530/30 nm Fluorescence Filter accessory has picture
7092167 532/40 nm Fluorescence Filter accessory has picture
7092168 540/15 nm Fluorescence Filter accessory has picture
7082249 540/25 nm Fluorescence Filter accessory has picture
7082253 540/35 nm Fluorescence Filter accessory has picture
7092169 540/45 nm Fluorescence Filter accessory has picture

Miscellaneous

1210007 Gas Controller for CO2 control. Includes CO2 flow meter & tubing set (p/n 1213032) accessory has picture
1210008 Gas Controller for CO2 and O2 control. Includes CO2 and N2 flow meters & tubing set (p/n 1213033 and p/n 1213032). accessory has picture
1210011 Calibration Kit for CO2 Controller

Reagent Injector Assembly

8040003S Dual Reagent Injector Module accessory has picture

Reagent Injector Options

1320514 Angled tip injector head accessory has picture

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture
Take3TRIO Take3 Trio Micro-Volume Plate accessory has picture

Test Plates & Reagents

1400501 Fluorescence Test Plate accessory has picture
7260522E Absorbance Test Plate accessory has picture
7260551 340 nm Absorbance Test Plate accessory has picture
8030015 Luminescence Test Plate accessory has picture

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