MultiFlo FX Multi-Mode Dispenser

MultiFlo™ FX is an automated multi-mode reagent dispenser for 6- to 1536-well microplates offering BioTek’s unique Parallel Dispense™ technology. Up to four independent reagents can be dispensed in parallel without potential carryover. MultiFlo FX becomes a versatile multi-mode dispenser with the addition of either the RAD™ technology for random access dispensing to 6- to 384-well plates or a wash module for 6- to 384-well plate washing. Fast, intuitive programming and operation are via the color touch screen user interface. A MultiFlo FX configured with either RAD technology or the wash module replaces up to five liquid handlers.
 

Save BIG on reagent costs with New RAD technology!

In this example, a savings of 34% per plate was acquired.

SAVE up to 34% on reagent costs with RAD technology

 

Make the Switch Today! Trade in your Multidrop or WellMate for up to $5,000 towards a new MultiFlo FX

 
MultiFlo FX promo video

 
US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).
  • Multi-mode dispensing replaces up to four dispensers and a washer
  • RAD™ technology for dispensing to individual wells
  • Parallel Dispense™ technology:  peristaltic or syringe dispensing with no carryover
  • Cost savings through dispense mode options and low prime volumes
  • Cell friendly angled dispense and wash tubes, adjustable flow rates
  • Flexible experiment design with total control of dispense patterns
  • Large, high resolution color touch screen display
  • Onboard computer with two USB flash drive ports for file transfer, storage and operation
  • Optional plate washing module for additional functionality
  • Modular and upgradable
  • ELISAs, cell assays and superparamagnetic bead based assays such as Luminex xMAP®
  • Compact footprint saves space and improves efficiency
  • Wide array of plate formats up to 50 mm in height
  • BioStack™ 4 compatible to automate cell-based workflows using  lidded plates
MultiFlo FX with WASH module

MultiFlo FX with RAD
  MFX MFXP* MFXW** MFXPW** MFXR*** MFXPR***
Unconfigured base instrument
         
1 peripump dispenser only  
       
Wash module only    
     
1 peripump dispenser and wash module      
   
RAD technology with one peripump          
RAD technology with two peripumps          

 

*P configurations include one plastic tip cassette
**W configurations include 96/384 wash manifold
***R configurations include one plastic tip multi-channel or RAD cassette.


 

Dispenser Modules (purchased separately)

7210010 - External Peristaltic Pump Dispenser Module
Adds 1 peristaltic pump dispenser to MultiFlo FX base instruments. For 6- to 1536-well microplates. Includes 1 plastic tip cassette.

7210008 - External Dual Syringe Dispenser Module, Autoclavable
Adds 2 syringe drive dispensers to MultiFlo FX base instruments.

7210009 - External Dual Syringe Dispenser Module, Non-Autoclavable
Adds 2 syringe drive dispensers to MultiFlo FX base instruments.

 

Note: See accessories tab for all available accessories.

 

General
User Interface 5.7" LCD touch screen display
USB ports (2) For protocol storage/transfer and for optional external mouse or keyboard
Shaking Slow, medium, fast or variable up to 60 minutes
Soak Time Programmable in minutes and seconds, up to 60 minutes
Microplate Types Dispensing (peri pump and syringe)
96-, 384- and 1536-well standard, deep and PCR plates; 6-. 12-, 24- and 48-well plates (dispense tip configurable)
Washing
96-. 384-well standard plates; 6-, 12-, 24-,and 48-well plates (with compatible manifold configuration)
Safety/Convenience/Maintenance Adjustment utility for plate positioning
Pre-programmed maintenance routines
Automation Compatible with BioStack and 3rd party automation
Onboard Software Create, edit or run multiple protocols, extensive Help system
Software Liquid Handling Control™, for PC protocol programming and execution (optional)
Labware Types Multi-channel peristaltic and syringe dispensing: 6-, to 1536-well microplates; low profile, standard height and deep well formats, PCR trays, microtubes. Max. height: 47 or 50 mm (carrier dependent) RAD dispensing: Single tip; 6-, to 384-well plates; low profile, standard height and deep well formats; PCR trays and microtubes. 8-to-1 tip; 6-, 12-, and 24-well plates
Washing: 6- to 384-well standard plates (with appropriate manifold)
Sterilization Peristaltic and syringe pump dispensers: Autoclave, up to 50 cycles at 121° C and 1 bar (750 mmHg) or chemical, 70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Washer: Chemical, 70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Humidity Maximum 80% relative humidity at temperatures up to 31° C (87.8° F) decreasing linearly to 50% at 40° C (104° F)
Electrical Compatible with 100 - 240 VAC ±10% 50-60 Hz
Washing
Manifold Types 96-/384-well plate washing: 1x8 manifold; 6-, 12-, 24-, 48-well plate washing: Custom manifolds available
Washing Speed 3 asp. /disp. cycles: >300 µL/well, 96 wells, 8-tube manifold, final aspirate <130 seconds
Fluid Delivery One positive displacement syringe pump
Volume Range 20-30,000 µL/well
Flow Rates 140-422 µL/well/second
Wash Cycles 1-10
Dispense Precision <3% CV (96-/384-well plates; 300 µL/well) and (12-/48-well plates; volume dependent)
<5% CV (6-well plates; 5560 µL/well)
Dispense Accuracy +3%
Residual Volume <2 µL/well, 300 µL dispense, 0.1% Tween
Sterilization Chemical: 70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Supply Bottle 2 L
Dispensing - Peristaltic pump (Multi-Channel)
Manifold Types 1 x 8 - sapphire jeweled 316 SS, 316 SS or polypropylene tips, with 1, 5 or 10 µL tubing
Dispense Speed 1 µL cass, 1 µL/ well: 6s (384 wells), 21s (1536 wells)
5 µL cass, 5 µL/ well: 6.5s (384 wells)
5 µL cass, 10 µL/ well: 3s (96 wells)
5 µL cass, 20 µL/ well: 3.5s (96 wells)
10 µL cass, 10 µL/ well: 8s (384 wells)
Volume Range 500 nL - 3,000 µL/well, selectable in 1 µL increments
Fluid Delivery One or two positive displacement peristaltic pump
Flow Rates Adjustable high flow to very low flow rates
Minimum Prime Volume 1 µL cass, 18": 0.78 mL; 1 µL cass, 30": 1.20 mL
5 µL cass, 18": 2.75 mL; 1 µL cass, 30": 4.23 mL
10 µL cass, 18": 4.79 mL; 10 µL cass, 30": 7.36 mL
Cassette Size 1 µL
Recommended Volume Range: 1 - 50 µL
Dispense Accuracy: at 1 µL ±5%
Dispense Precision: at 1 µL <5% CV
Dispense Precision: at 500 nL < 10% CV
Minimum Prime Volume: 0.78 - 1.20 mL

5 µL
Recommended Volume Range: 5 - 2,500 µL
Dispense Accuracy: at 5 µL ±2%
Dispense Precision: at 5 µL <2.5% CV
Minimum Prime Volume: 2.75 - 4.23 mL

10 µL
Recommended Volume Range: 10 - 3,000 µL
Dispense Accuracy: at 10 µL ±2%
Dispense Precision: at 10 µL <2% CV
Minimum Prime Volume: 4.79 - 7.36 mL
Dispense Performance 1 µL cass, 1-50 µL: Accuracy <5% at 1 µL, Precision <5% CV at 1 µL
1 µL cass, 1-50 µL: Precision < 10% CV at 500 nL
5 µL cass, 5-2,500 µL: Accuracy +2% at 5 µL, Precision <2.5% CV at 5 µL
10 µL cass, 10-3,000 µL: Accuracy +2% at 10 µL, Precision <2% CV at
10 µL
Recommended Replacement Interval 1 µL Cassette: 1,000 384-well microplates at 5 µL well
5 µL Cassette: 1,000 96-well microplates at 50 µL well
10 µL Cassette: 1,000 96-well microplates at 100 µL well
Sterilization Autoclave:
Up to 50 cycles at temperatures and pressures of 121° C and 1 bar (750 mmHg)

Chemical:
70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Dispensing - Peristaltic pump (RAD Technology)
Labware Types Single tip: 6-, 12-, 24-, 48-, 96-, 384-well plates; low profile, standard height and deep well formats; PCR trays and microtubes
8-to-1 tip: 6-, 12-, and 24-well plates
Manifold Types RAD single, with plastic or steel tip with 1, 5 or 10 tubing, 7° angle
RAD 8-to-1 plastic tip, with 5 µL tubing, angled bulk dispense chute
Volume Range 500 nL - 30,000 µL
Minimum Prime Volume 1 µL cass, 18": 90 µL ; 1 µL cass, 30": 150 µL
5 µL cass, 18": 320 µL; 1 µL cass, 30": 530 µL
10 µL cass, 18": 555 µL; 10 µL cass, 30": 920 µL
Dispense Speed (high flow) 1 µL cass, 1 µL/well: 19s (96 wells) 55s (384 wells)
1 µL cass, 10 µL/well: 33s (96 wells), 112s (384 wells)
5 µL cass, 5 µL/well: 19s (96 wells), 58s (384 wells)
5 µL cass, 100 µL/well: 76s (96 wells), 286s (384 wells)
10 µL cass, 10 µL/well: 21s (96 wells), 66s (384 wells)
10 µL cass, 100 µL/well: 70s (96 wells), 259s (384 wells)
Dispense Performance 1 µL cass (med), 0.5 µL/well: Precision 10% CV
1 µL cass (med), 1 µL/well: Accuracy + 10%, Precision 10% CV
1 µL cass (med), >2 µL/well: Accuracy + 5%, Precision 5% CV
5 µL cass (high), 5 µL/well: Accuracy + 4%, Precision 5% CV
5 µL cass (high), >10 µL/well: Accuracy + 2%, Precision 2.5% CV
10 µL cass (high), 10 µL/well: Accuracy + 4%,Precision 4% CV
10 µL cass (high), >20 µL/well: Accuracy + 2%, Precision 2 %CV
8-to 1 cass(high), 40 µL/well: Accuracy + 4%
8-to 1 cass (high), >10 µL/well: Precision 2.5%CV
8- to 1cass(high), >80 µL/well: Accuracy + 2%
Dispense Accuracy +10% (1 µL cassette (med flow rate); 1 µL/well)
+5% (1 µL cassette (med flow rate); >2 µL/well)

+4% (5 µL cassette; 5 µL/well)
+2% (5 µL cassette; >10 µL/well)

+4% (10 µL cassette; 10 µL/well)
+2% (10 µL cassette; >20 µL/well)

+4% (8-to-1 cassette; 40 µL/well)
+2% (8-to-1 cassette; >80 µL/well)
Dispense Precision 10% CV (1 µL cassette (med flow rate); 0.5 µL/well)
10% CV (1 µL cassette (med flow rate); 1 µL/well)
5% CV (1 µL cassette (med flow rate); >2 µL/well)
 
5% CV (5 µL cassette; 5 µL/well)
2.5% CV (5 µL cassette; >10 µL/well)
 
4% CV (10 µL cassette; 10 µL/well)
2% CV (10 µL cassette; >20 µL/well)
2.5% CV (8-to-1 cassette; >10 µL/well)
Dispensing - Syringe pump
Manifold Types 96-/384-well dispensing: (1) 2 x 8 manifold, 316 SS tubes, autoclavable and non-autoclavable available. (2) 1 x 16 manifolds, 316 SS tubes, autoclavable
1536-well dispensing: (2) 1 x 32 manifolds sapphire jeweled 316 SS SB or 316 SS LB tubes, autoclavable
6-, to 48-well dispensing: Custom autoclavable manifolds available for each plate type
Dispense Speed 20 µL /well, 96 wells, 1 x16: 5 seconds
20 µL /well, 384 wells, 1 x16: 14 seconds
3 µL /well, 1536 wells, 2 x 32: 7 seconds
Volume Range 3 - 3,000 µL/well (manifold dependent)
Selectable in 1 µL increments
Fluid Delivery Two positive displacement syringe drives
Dispense Performance 5 µL/well: Accuracy + 1 µL, Precision < 5% CV
20 µL/well: Accuracy + 1µL, Precision < 2.5% CV
100 µL/well: Accuracy + 1 %, Precision < 1% CV
Minimum Prime Volume 12 mL
Dispense Accuracy +1 µL at 5 µL
+1 µL at 20 µL
+1% at 100 µL
Dispense Precision <5% CV at 5 µL
<2.5% CV at 20 µL
<1% CV at 100 µL
Supply Bottle 1 L or 2 L (model dependent)
Physical Characteristics
Power 100 - 240 Volts AC. 50/60 Hz.
Dimensions Base instrument: 17.19"W x 11.75" D x 8" H (43.51 x 29.21 x 20.32 cm)
Weight Base instrument:
19.5 lbs (8.8 Kg)
Regulatory
Regulatory CE and TUV marked, RoHS Compliant. Models for In Vitro Diagnostic use are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Liquid Handling Control™ Software

Liquid Handling Control™ Software

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.


Optional

BioStack Integration Kits

7310044 BioStack Integration Kit for MultiFlo FX & MultiFlo

Bottles & Tubing

48140 Waste Bottle (2L) without cap accessory has picture
4070515 Dispense Bottle (2L) with Cap and Connector accessory has picture
4070545S Waste Bottle Level Sense Assembly
7140511 Autoclavable Reagent Bottle (1L) with Cap and Connector accessory has picture
7180541 Dispense Tubing Set - Teflon Lined
7183002 Supply Tubing Set for DMSO & Acetonitrile for 2 Syringe Pump Dispensers

Cassettes

1260015 10 - 3,000 µL, 10 µL increments, RAD peri cassette, plastic tip
1260016 5- 2,500 µL, 5 µL increments, RAD peri cassette, plastic tip
1260017 500 nL- 50 µL, 1 µL increments, RAD peri cassette, plastic tip
1260018 10 - 3,000 µL, 10 µL increments, RAD peri cassette, steel tip
1260019 5 - 2,500 µL, 5 µL increment, RAD peri cassette, steel
1260020 10 - 3,000 µL, 10 µL increments, RAD peri cassette, plastic tip, large bore
1260021 500 nL - 50 µL, 1 µL increments, RAD peri cassette, steel tip
1260022 8 µL - 30,000 µL, RAD peri cassette, 8-to-1 tube/tip, plastic tip
7170007 Large Volume Replacement Tubing Set (10 µL) - molded plastic tips accessory has picture
7170008 Medium Volume Replacement Tubing Set (5 µL) - molded plastic tips accessory has picture
7170009 Low Volume Replacement Tubing Set (1 µL) - molded plastic tips accessory has picture
7170010 Large Volume (10 µL) Cassette - molded plastic tips accessory has picture
7170011 Medium Volume (5 µL) Cassette - molded plastic tips accessory has picture
7170012 Low Volume (1 µL) Cassette - molded plastic tips accessory has picture
7170013 Large Volume (10 µL) Cassette - stainless steel tips accessory has picture
7170014 Medium Volume (5 µL) Cassette - stainless steel tips accessory has picture
7170015 Low Volume (1 µL) Cassette - sapphire jeweled, stainless steel tips accessory has picture
7170016 Low Volume (1 µL) 1536-well Cassette accessory has picture
7170018 Low Volume (1 µL) 1536-well Cassette - molded plastic tips accessory has picture
7170020 Large volume (10 µL) Cassette Tubing Extension Kit accessory has picture
7170021 Medium volume (5 µL) Cassette Tubing Extension Kit accessory has picture
7170022 Low volume (1 µL) Cassette Tubing Extension Kit accessory has picture
7170023 Large Volume (10 µL) Cassette - large bore, molded plastic tips
7170024 Medium Volume (5 µL) Cassette - large bore, molded plastic tips

Labware Holders

7212042 Minitube Carrier - for labware up to 50 mm in height, including 1.2 mL minitubes accessory has picture

Manifolds

1260009 Wash Manifold Set, 6-well Plates
1260010 Wash Manifold Set, 12-well Plates
1260011 Wash Manifold Set, 24-well Plates
1260012 Wash Manifold Set, 48-well Plates
1260534S Syringe manifold, 48- well plates, autoclavable
1260535S Syringe manifold, 24-well plates, autoclavable
1260536S Syringe manifold, 12-well plates, autoclavable
1260537S Syringe manifold, 6-well plates, autoclavable
7180543S Syringe Dispenser Manifold, 96-/384-well Plates - 1x16-tube, Autoclavable
7180548S Syringe Dispenser Manifold, 96-/384-well Plates - 2x8-tube, Non-Autoclavable
7180552S Syringe Dispenser Manifold, 1536-well Plates - 1x32-tube Large Bore, Autoclavable
7180553S Syringe Dispenser Manifold, 1536-well Plates - 1x32-tube Small Bore, Autoclavable
7180560S Syringe Dispenser Manifold, 96-/384-well Plates - 2x8-tube, Autoclavable

Miscellaneous

1260023 MultiFlo FX Washer Upgrade Kit
7102215 384-well Ring Magnet accessory has picture
7102216 96-well Ring Magnet accessory has picture
7103016 96-well Flat Magnet accessory has picture
7103017 384-well Flat Magnet accessory has picture
7210509 Spare Reservoir Holder and Strap
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture

Product Qualification Packages

1260521 MultiFlo FX Product Qualification Package

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