MultiFlo FX Multi-Mode Dispenser

MultiFlo™ FX is an automated multi-mode reagent dispenser for 6- to 1536-well microplates offering BioTek’s unique Parallel Dispense™ technology. Up to four independent reagents can be dispensed in parallel without potential carryover. MultiFlo FX becomes a versatile multi-mode dispenser with the addition of either the RAD™ technology for random access dispensing to 6- to 384-well plates or a wash module for 6- to 384-well plate washing. Fast, intuitive programming and operation are via the color touch screen user interface. A MultiFlo FX configured with either RAD technology or the wash module replaces up to five liquid handlers.
 

Save BIG on reagent costs with New RAD technology!

In this example, a savings of 34% per plate was acquired.

SAVE up to 34% on reagent costs with RAD technology

 

See the MultiFlo FX in Action

 
MultiFlo FX video
  • Multi-mode dispensing replaces up to four dispensers and a washer
  • RAD™ technology for dispensing to individual wells
  • Parallel Dispense™ technology:  peristaltic or syringe dispensing with no carryover
  • Cost savings through dispense mode options and low prime volumes
  • Cell friendly angled dispense and wash tubes, adjustable flow rates
  • Flexible experiment design with total control of dispense patterns
  • Large, high resolution color touch screen display
  • Onboard computer with two USB flash drive ports for file transfer, storage and operation
  • Optional plate washing module for additional functionality
  • Modular and upgradable
  • ELISAs, cell assays and superparamagnetic bead based assays such as Luminex xMAP®
  • Compact footprint saves space and improves efficiency
  • Wide array of plate formats up to 50 mm in height
  • BioStack™ 4 compatible to automate cell-based workflows using  lidded plates
MultiFlo FX with WASH module

MultiFlo FX with RAD
  MFX MFXP* MFXW** MFXPW** MFXR*** MFXPR***
Unconfigured base instrument
         
1 peripump dispenser only  
       
Wash module only    
     
1 peripump dispenser and wash module      
   
RAD technology with one peripump          
RAD technology with two peripumps          

 

*P configurations include one plastic tip cassette
**W configurations include 96/384 wash manifold
***R configurations include one plastic tip multi-channel or RAD cassette.


 

Dispenser Modules (purchased separately)

7210010 - External Peristaltic Pump Dispenser Module
Adds 1 peristaltic pump dispenser to MultiFlo FX base instruments. For 6- to 1536-well microplates. Includes 1 plastic tip cassette.

7210008 - External Dual Syringe Dispenser Module, Autoclavable
Adds 2 syringe drive dispensers to MultiFlo FX base instruments.

7210009 - External Dual Syringe Dispenser Module, Non-Autoclavable
Adds 2 syringe drive dispensers to MultiFlo FX base instruments.

 

Note: See accessories tab for all available accessories.

 

General
User Interface 5.7" LCD touch screen display
Microplate Types Dispensing (peri pump and syringe)
96-, 384- and 1536-well standard, deep and PCR plates; 6-. 12-, 24- and 48-well plates (dispense tip configurable)
Washing
96-. 384-well standard plates; 6-, 12-, 24-,and 48-well plates (with compatible manifold configuration)
USB ports (2) For protocol storage and transfer
For optional external mouse or keyboard
Shaking Programmable in minutes and seconds, up to 60 minutes
Intensities - slow, medium, fast or variable
Soak Time Programmable in minutes and seconds, up to 60 minutes
Safety/Convenience/Maintenance Adjustment utility for plate positioning
Pre-programmed maintenance routines
Automation Compatible with BioStack and 3rd party automation
Onboard Software Create, edit or run multiple protocols, extensive Help system
Software Liquid Handling Control™, for PC protocol programming and execution (optional)
Operating Temperature 15° C to 35° C (59° F to 95° F)
Humidity Maximum 80% relative humidity at temperatures up to 31° C (87.8° F) decreasing linearly to 50% at 40° C (104° F)
Electrical Compatible with 100 - 240 VAC ±10% 50-60 Hz
Washing
Manifold Types 96-/384-well washing:
8-tube (1x8) manifold

6-, 12-, 24-, 48-well washing:
Custom manifolds available
Washing Speed 3 asp. /disp. cycles: >300 µL/well, 96 wells, 8-tube manifold, final aspirate <105 seconds
Fluid Delivery One positive displacement syringe pump
Volume Range 20-30,000 µL/well
Flow Rates 1-9
Wash Cycles 1-10
Dispense Accuracy +3%
Dispense Precision <3% CV (96-/384-well plates; 300 µL/well)
<3% CV (12-/48-well plates; volume dependent)
<5% CV (6-well plates; 5560 µL/well)
Residual Volume <2 µL/well, 300 µL dispense, 0.1% Tween
Sterilization Chemical: 70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Supply Bottle Volume 2 L
Dispensing - Peristaltic pump (Multi-Channel)
Manifold Types 8-tip cassette (1 x 8) - sapphire jeweled 316 stainless steel, 316 stainless steel or polypropylene plastic tips
Labware Types 6-, 12-, 24-, 48-, 96-, 384- and 1536-well microplates in low profile, standard height and deep well formats (manifold dependent)
PCR trays
Microtubes
Maximum height: 47 or 50 mm (carrier dependent)
Dispense Speed 10 µL/well, 384 wells: 8 secs (10 µL cassette)
10 µL/well, 96 wells: 3 secs (5 µL cassette)
5 µL/well, 384 wells: 6.5 secs (5 µL cassette)
1 µL/ well, 1536 wells: 21 secs (1 µL cassette)
Volume Range 500 nL - 3,000 µL/well
Selectable in 1 µL increments
Fluid Delivery One or two positive displacement peristaltic pump
Flow Rates Low, medium or high
Cassette Size 1 µL
Recommended Volume Range: 1 - 50 µL
Dispense Accuracy: at 1 µL ±5% typical
Dispense Precision: at 1 µL <5% CV typical
Dispense Precision: at 500 nL < 10% CV typical
Minimum Prime Volume: 0.78 - 1.20 mL

5 µL
Recommended Volume Range: 5 - 2,500 µL
Dispense Accuracy: at 5 µL ±2% typical
Dispense Precision: at 5 µL <2.5% CV typical
Minimum Prime Volume: 2.75 - 4.23 mL

10 µL
Recommended Volume Range: 10 - 3,000 µL
Dispense Accuracy: at 10 µL ±2% typical
Dispense Precision: at 10 µL <2% CV typical
Minimum Prime Volume: 4.79 - 7.36 mL
Recommended Replacement Interval 1 µL Cassette: 1,000 384-well microplates at 5 µL well
5 µL Cassette: 1,000 96-well microplates at 50 µL well
10 µL Cassette: 1,000 96-well microplates at 100 µL well
Sterilization Autoclave:
Up to 50 cycles at temperatures and pressures of 121° C and 1 bar (750 mmHg)

Chemical:
70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Dispensing - Peristaltic pump (RAD Technology)
Labware Types Single tip: 6-, 12-, 24-, 48-, 96-, 384-well plates; low profile, standard height and deep well formats; PCR trays and microtubes
8-to-1 tip: 6-, 12-, and 24-well plates
Manifold Types RAD single, with plastic or steel tip with 1, 5 or 10 tubing, 7° angle
RAD 8-to-1 plastic tip, with 5 µL tubing, angled bulk dispense chute
Volume Range 500 nL - 30,000 µL
Minimum Prime Volume 1 µL cassette
Reduced tube length (18") - 90 µL
Standard tube length (30") - 150 µL

5 µL cassette
Reduced tube length (18") - 320 µL
Standard tube length (30") - 530 µL

10 µL cassette
Reduced tube length (18") - 555 µL
Standard tube length (30") - 920 µL
Dispense Speed (flow rate high) 1 µL/well, 96 wells: 19 secs (1 µL cassette)
10 µL/well, 96 wells: 33 secs (1 µL cassette)
5 µL/well, 96 wells: 19 secs (5 µL cassette)
100 µL/ well, 96 wells: 76 secs (5 µL cassette)
10 µL/well, 96 wells: 21 secs (10 µL cassette)
100 µL/well, 96 wells: 70 secs (10 µL cassette)

1 µL/well, 384 wells: 55 secs (1 µL cassette)
10 µL/well, 384 wells: 112 secs (1 µL cassette)
5 µL/well, 384 wells: 58 secs (5 µL cassette)
100 µL/ well, 384 wells: 286 secs (5 µL cassette)
10 µL/well, 384 wells: 66 secs (10 µL cassette)
100 µL/well, 384 wells: 259 secs (10 µL cassette)
Dispense Accuracy +10% (1 µL cassette (med flow rate); 1 µL/well)
+5% (1 µL cassette (med flow rate); >2 µL/well)

+4% (5 µL cassette; 5 µL/well)
+2% (5 µL cassette; >10 µL/well)

+4% (10 µL cassette; 10 µL/well)
+2% (10 µL cassette; >20 µL/well)

+4% (8-to-1 cassette; 40 µL/well)
+2% (8-to-1 cassette; >80 µL/well)
Dispense Precision 10% CV (1 µL cassette (med flow rate); 0.5 µL/well)
10% CV (1 µL cassette (med flow rate); 1 µL/well)
5% CV (1 µL cassette (med flow rate); >2 µL/well)
 
5% CV (5 µL cassette; 5 µL/well)
2.5% CV (5 µL cassette; >10 µL/well)
 
4% CV (10 µL cassette; 10 µL/well)
2% CV (10 µL cassette; >20 µL/well)
2.5% CV (8-to-1 cassette; >10 µL/well)
Dispensing - Syringe pump
Manifold Types 96- / 384-well dispensing:
One 16-tube (2 x 8) manifold - 316 stainless steel tubes

96- / 384-well dispensing:
Two 16-tube (1 x 16) manifolds - 316 stainless steel tubes

1536-well dispensing:
Two 32-tube (1 x 32) manifolds - sapphire jeweled 316 stainless steel SB or 316 stainless steel LB tubes
Labware Types 96-, 384- and 1536-well microplates in low profile, standard height and deep well formats (manifold dependent)
PCR trays
Microtubes
Maximum height: 47 or 50 mm (carrier dependent)
Dispense Speed 20 µL /well, 96 wells, 1 x16: 5 seconds
20 µL /well, 384 wells, 1 x16: 14 seconds
3 µL /well, 1536 wells, 1 x 32: 14 seconds
3 µL /well, 1536 wells, 2 x 32: 7 seconds
Volume Range 3 - 3,000 µL/well (manifold dependent)
Selectable in 1 µL increments
Fluid Delivery 2 microprocessor-controlled syringe drives
Flow Rates 1 - 5
Dispense Accuracy +1 µL typical at 5 µL
+1 µL typical at 20 µL
+1% typical at 100 µL
Dispense Precision <5% CV typical at 5 µL
<2.5% CV typical at 20 µL
<1% CV typical at 100 µL
Supply Bottle Volume 1 L or 2 L (model dependent)
Sterilization Autoclave: (model dependent)
Up to 50 cycles at temperatures and pressures of 121° C and 1 bar (750 mmHg)

Chemical:
70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Physical Characteristics
Dimensions Base instrument:
Depth: 11.75" (29.21 cm)
Width: 17.19" (43.51 cm)
Height: 8.0" (20.32 cm)
Weight Base instrument:
19.5 lbs (8.8 Kg)
Regulatory
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available. RoHS Compliant.

Specifications are subject to change. Performance values represent the average observed factory test values.

Optional

BioStack Integration Kits

7310044 BioStack Integration Kit for MultiFlo FX & MultiFlo

Bottles & Tubing

48140 Waste Bottle (2L) without cap accessory has picture
4070515 Dispense Bottle (2L) with Cap and Connector accessory has picture
4070545S Waste Bottle Level Sense Assembly
7140511 Autoclavable Reagent Bottle (1L) with Cap and Connector accessory has picture
7180541 Dispense Tubing Set - Teflon Lined
7183002 Supply Tubing Set for DMSO & Acetonitrile for 2 Syringe Pump Dispensers

Cassettes

1260015 10 - 3,000 µL, 10 µL increments, RAD peri cassette, plastic tip
1260016 5- 2,500 µL, 5 µL increments, RAD peri cassette, plastic tip
1260017 500 nL- 50 µL, 1 µL increments, RAD peri cassette, plastic tip
1260018 10 - 3,000 µL, 10 µL increments, RAD peri cassette, steel tip
1260019 5 - 2,500 µL, 5 µL increment, RAD peri cassette, steel
1260020 10 - 3,000 µL, 10 µL increments, RAD peri cassette, plastic tip, large bore
1260021 500 nL - 50 µL, 1 µL increments, RAD peri cassette, steel tip
1260022 8 µL - 30,000 µL, RAD peri cassette, 8-to-1 tube/tip, plastic tip
7170007 Large Volume Replacement Tubing Set (10 µL) - molded plastic tips accessory has picture
7170008 Medium Volume Replacement Tubing Set (5 µL) - molded plastic tips accessory has picture
7170009 Low Volume Replacement Tubing Set (1 µL) - molded plastic tips accessory has picture
7170010 Large Volume (10 µL) Cassette - molded plastic tips accessory has picture
7170011 Medium Volume (5 µL) Cassette - molded plastic tips accessory has picture
7170012 Low Volume (1 µL) Cassette - molded plastic tips accessory has picture
7170013 Large Volume (10 µL) Cassette - stainless steel tips accessory has picture
7170014 Medium Volume (5 µL) Cassette - stainless steel tips accessory has picture
7170015 Low Volume (1 µL) Cassette - sapphire jeweled, stainless steel tips accessory has picture
7170016 Low Volume (1 µL) 1536-well Cassette accessory has picture
7170018 Low Volume (1 µL) 1536-well Cassette - molded plastic tips accessory has picture
7170020 Large volume (10 µL) Cassette Tubing Extension Kit accessory has picture
7170021 Medium volume (5 µL) Cassette Tubing Extension Kit accessory has picture
7170022 Low volume (1 µL) Cassette Tubing Extension Kit accessory has picture
7170023 Large Volume (10 µL) Cassette - large bore, molded plastic tips
7170024 Medium Volume (5 µL) Cassette - large bore, molded plastic tips

Labware Holders

7212042 Minitube Carrier - for labware up to 50 mm in height, including 1.2 mL minitubes accessory has picture

Manifolds

1260009 Wash Manifold Set, 6-well Plates
1260010 Wash Manifold Set, 12-well Plates
1260011 Wash Manifold Set, 24-well Plates
1260012 Wash Manifold Set, 48-well Plates
1260534S Syringe manifold, 48- well plates, autoclavable
1260535S Syringe manifold, 24-well plates, autoclavable
1260536S Syringe manifold, 12-well plates, autoclavable
1260537S Syringe manifold, 6-well plates, autoclavable
7180543S Syringe Dispenser Manifold, 96-/384-well Plates - 1x16-tube, Autoclavable
7180548S Syringe Dispenser Manifold, 96-/384-well Plates - 2x8-tube, Non-Autoclavable
7180552S Syringe Dispenser Manifold, 1536-well Plates - 1x32-tube Large Bore, Autoclavable
7180553S Syringe Dispenser Manifold, 1536-well Plates - 1x32-tube Small Bore, Autoclavable
7180560S Syringe Dispenser Manifold, 96-/384-well Plates - 2x8-tube, Autoclavable

Miscellaneous

7102215 384-well Ring Magnet accessory has picture
7102216 96-well Ring Magnet accessory has picture
7103016 96-well Flat Magnet accessory has picture
7103017 384-well Flat Magnet accessory has picture
7140516 Spare Autoclavable Syringe Head with Piston
7210509 Spare Reservoir Holder and Strap
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture

Product Qualification Packages

1260521 MultiFlo FX Product Qualification Package

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