MultiFlo Microplate Dispenser
BioTek's new MultiFlo™ Microplate Dispenser is the latest advancement in bulk dispensers offering up to four reagents dispensed in parallel with one compact instrument. The MultiFlo offers a choice of either peristaltic pump or microprocessor controlled syringe drive technologies. Users can purchase one channel now and add others in the future. A wide array of plate types are accommodated from 6- to 1536-well formats. The MultiFlo dispenses a broad volume range from 500 nL to 3000 µL accurately and precisely with a fully modular and upgradable design. BioTek's proprietary angled dispensing ensures compatibility with all dispense protocols including reagent addition to loosely adherent cell monolayers. For assays requiring minimum reagent usage, the MultiFlo's prime volume is under 1 mL. A built-in priming trough is designed to allow both recovery of reagents or to send primed reagent directly to waste.
With a small footprint and base height of less than 8", the MultiFlo comfortably fits on any laboratory bench or robotic system. Easily integrated with a BioStack™ Microplate Stacker, up to 75 plates can be reliably dispensed by the MultiFlo with walkaway confidence. Alternatively, robotic integration of the MultiFlo to third party automated systems is seamless with available Liquid Handling Control™ Software. LHC™ additionally allows dispense protocols to be created and run from the PC or downloaded to the onboard user interface.
- Broad volume range from 500 nL to 3000 µL
- 6- to 1536-well microplates with a wide array of plate formats including low profile, standard height, deep well and microtubes
- Up to four independent reagents dispensed in parallel
- Available with one or two peristaltic pumps, two syringe pumps or any combination thereof
- Fully modular and upgradable design
- Speed: dispenses 384-wells in 6 seconds
- Compact footprint with base instrument height under 8"
- 7° angled dispensing for minimized disturbance to loosely adherent cell monolayers
- Autoclavable dispense paths for guaranteed sterility
- Confidence-Plus™ unconditional lifetime warranty
- Easy to use keypad interface or increased flexibility with LHC PC Software
- BioStack automation compatible
- Available 3-MultiFlo rack for robotic system integration
Instruments
MF - MultiFlo™ Dispenser
MultiFlo base instrument, unconfigured. For labware up to 47 mm in height.
MFP - MultiFlo™ Dispenser
MultiFlo base instrument, with 1 internal peristaltic pump dispenser. For 6- to 1536-well microplates and labware up to 47 mm in height. Includes 1 plastic tip cassette.
Dispenser Modules (purchased separately)
7210010 - External Peristaltic Pump Dispenser Module
Adds 1 peristaltic pump dispenser to MultiFlo base instruments. For 6- to 1536-well microplates. Includes 1 plastic tip cassette.
7210008 - External Dual Syringe Dispenser Module, Autoclavable
Adds 2 syringe drive dispensers to MultiFlo base instruments.
7210009 - External Dual Syringe Dispenser Module, Non-Autoclavable
Adds 2 syringe drive dispensers to MultiFlo base instruments.
Manifolds for Syringe Dispenser Modules (purchased separately)
7180548S - 2x8-tube Manifold, 96-/384-well Microplates
For 96- and 384-well dispensing, including half-area and PCR 96-well formats. Steel tips. 1 required for Dual Syringe Dispenser Module. Non-Autoclavable.
7180543S - 1x16-tube Manifold, 96-/384-well Microplates
For 96- and faster 384-well microplate dispensing. Steel tips. 2 required for Dual Syringe Dispenser Module.
7180552S
- 1x32-tube Large Bore Manifold, 1536-well Microplates
For 1536-well microplate dispensing. Steel tips. Recommended with most liquids – those not containing surfactant. 2 required for Dual Syringe Dispenser Module.
7180553S - 1x32-tube Small Bore Manifold, 1536-well Microplates
For 1536-well microplate dispensing. Sapphire steel tips. Recommended with all liquids. 2 required for Dual Syringe Dispenser Module.
Note: P/N MFP and 7210010 each include any 1 plastic tip dispense cassette.
| General | |
| Number of Reagents | 1 - 4 (model dependent) |
| Shaking | Programmable in minutes and seconds, up to 60 minutes Intensities - slow, medium, fast or variable |
| Soak Time | Programmable in minutes and seconds, up to 60 minutes Safety/Convenience/Maintenance Adjustment utility for plate positioning Pre-programmed maintenance routines |
| Automation | BioStack™ Microplate Stacker - up to 50 SBS standard height plates (optional) |
| Onboard Software | 2 x 24 character LCD display 25 alphanumeric soft keys Create, edit or run multiple protocols |
| Software | Liquid Handling Control™, for PC protocol programming and execution (optional) |
| Operating Temperature | 15° C to 35° C (59° F to 95° F) |
| Humidity | Maximum 80% relative humidity at temperatures up to 31° C (87.8° F) decreasing linearly to 50% at 40° C (104° F) |
| Electrical | Compatible with 100 - 240 VAC ±10% 50-60 Hz |
| Dispensing - Peristaltic pump | |
| Manifold Types | 8-tip cassette (1 x 8) - sapphire jeweled 316 stainless steel, 316 stainless steel or polypropylene plastic tips |
| Labware Types | 6-, 12-, 24-, 48-, 96-, 384- and 1536-well microplates in low profile, standard height and
deep well formats (manifold dependent) PCR trays Microtubes Maximum height: 47 or 50 mm (carrier dependent) |
| Dispensing Speed | 10 µL /well, 96 wells, 8-tip cassette: 3 seconds 5 µL /well, 384 wells, 8-tip cassette: 6 seconds 1 µL /well, 1536 wells, 8-tip cassette: 18 seconds 500 nL /well, 1536 wells, 8-tip cassette: 15 seconds 500 nL /well, 384 wells, 8-tip cassette: 6 seconds |
| Volume Range | 500 nL - 3,000 µL/well Selectable in 1 µL increments |
| Fluid Delivery | One or two positive displacement peristaltic pump |
| Flow Rates | Low, medium or high |
| Cassette Size | 1 µL Recommended Volume Range: 1 - 50 µL Dispense Accuracy: at 1 µL ±5% typical Dispense Precision: at 1 µL <5% CV typical Dispense Precision: at 500 nL < 10% CV typical Minimum Prime Volume: 0.78 - 1.20 mL 5 µL Recommended Volume Range: 5 - 2,500 µL Dispense Accuracy: at 5 µL ±2% typical Dispense Precision: at 5 µL <2.5% CV typical Minimum Prime Volume: 2.75 - 4.23 mL 10 µL Recommended Volume Range: 10 - 3,000 µL Dispense Accuracy: at 10 µL ±2% typical Dispense Precision: at 10 µL <2% CV typical Minimum Prime Volume: 4.79 - 7.36 mL |
| Recommended Replacement Interval | 1 µL Cassette: 1,000 384-well microplates at 5 µL well 5 µL Cassette: 1,000 96-well microplates at 50 µL well 10 µL Cassette: 1,000 96-well microplates at 100 µL well |
| Sterilization | Autoclave: Up to 50 cycles at temperatures and pressures of 121° C and 1 bar (750 mmHg) Chemical: 70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution |
| Dispensing - Syringe pump | |
| Minimum Prime Volume | 12 mL |
| Manifold Types | 96- / 384-well dispensing: One 16-tube (2 x 8) manifold - 316 stainless steel tubes 96- / 384-well dispensing: Two 16-tube (1 x 16) manifolds - 316 stainless steel tubes 1536-well dispensing: Two 32-tube (1 x 32) manifolds - sapphire jeweled 316 stainless steel SB or 316 stainless steel LB tubes |
| Labware Types | 96-, 384- and 1536-well microplates in low profile, standard height and
deep well formats (manifold dependent) PCR trays Microtubes Maximum height: 47 or 50 mm (carrier dependent) |
| Dispensing Speed | 20 µL /well, 96 wells, 1 x16: 5 seconds 20 µL /well, 384 wells, 1 x16: 14 seconds 3 µL /well, 1536 wells, 1 x 32: 14 seconds 3 µL /well, 1536 wells, 2 x 32: 7 seconds |
| Volume Range | 3 - 3,000 µL/well (manifold dependent) Selectable in 1 µL increments |
| Fluid Delivery | 2 microprocessor-controlled syringe drives |
| Flow Rates | 1 - 5 |
| Dispense Accuracy | +1 µL typical at 5 µL +1 µL typical at 20 µL +1% typical at 100 µL |
| Dispense Precision | <5% CV typical at 5 µL <2.5% CV typical at 20 µL <1% CV typical at 100 µL |
| Supply Bottle Volume | 1 L or 2 L (model dependent) |
| Sterilization | Autoclave: (model dependent) Up to 50 cycles at temperatures and pressures of 121° C and 1 bar (750 mmHg) Chemical: 70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution |
| Physical Characteristics | |
| Dimensions | Base instrument: Depth: 10.6" (26.9 cm) Width: 16.1" (40.9 cm) Height: 7.6" (19.3 cm) |
| Weight | Base instrument: 14.3 lbs (6.5 kg) |
| Regulatory | |
| Regulatory | CE and TUV marked. In Vitro Diagnostic use models are available. |
Specifications are subject to change.
Optional

BioStack3 Microplate Stacker

Liquid Handling Control Software
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