BioTek: Microplate Instrumentation & Software Solutions
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MultiFlo Microplate Dispenser

BioTek's new MultiFlo™ Microplate Dispenser is the latest advancement in bulk dispensers offering up to four reagents dispensed in parallel with one compact instrument. The MultiFlo offers a choice of either peristaltic pump or microprocessor controlled syringe drive technologies. Users can purchase one channel now and add others in the future. A wide array of plate types are accommodated from 6- to 1536-well formats. The MultiFlo dispenses a broad volume range from 500 nL to 3000 µL accurately and precisely with a fully modular and upgradable design. BioTek's proprietary angled dispensing ensures compatibility with all dispense protocols including reagent addition to loosely adherent cell monolayers. For assays requiring minimum reagent usage, the MultiFlo's prime volume is under 1 mL. A built-in priming trough is designed to allow both recovery of reagents or to send primed reagent directly to waste.

With a small footprint and base height of less than 8", the MultiFlo comfortably fits on any laboratory bench or robotic system. Easily integrated with a BioStack™ Microplate Stacker, up to 75 plates can be reliably dispensed by the MultiFlo with walkaway confidence. Alternatively, robotic integration of the MultiFlo to third party automated systems is seamless with available Liquid Handling Control™ Software. LHC™ additionally allows dispense protocols to be created and run from the PC or downloaded to the onboard user interface.

 

  • Broad volume range from 500 nL to 3000 µL
  • 6- to 1536-well microplates with a wide array of plate formats including low profile, standard height, deep well and microtubes
  • Up to four independent reagents dispensed in parallel
  • Available with one or two peristaltic pumps, two syringe pumps or any combination thereof
  • Fully modular and upgradable design
  • Speed: dispenses 384-wells in 6 seconds
  • Compact footprint with base instrument height under 8"
  • 7° angled dispensing for minimized disturbance to loosely adherent cell monolayers
  • Autoclavable dispense paths for guaranteed sterility
  • Confidence-Plus™ unconditional lifetime warranty
  • Easy to use keypad interface or increased flexibility with LHC PC Software
  • BioStack automation compatible
  • Available 3-MultiFlo rack for robotic system integration

Instruments

MF - MultiFlo Dispenser
MultiFlo base instrument, unconfigured. For labware up to 47 mm in height.

MFP - MultiFlo Dispenser
MultiFlo base instrument, with 1 internal peristaltic pump dispenser. For 6- to 1536-well microplates and labware up to 47 mm in height. Includes 1 plastic tip cassette.



Dispenser Modules (purchased separately)

7210010 - External Peristaltic Pump Dispenser Module
Adds 1 peristaltic pump dispenser to MultiFlo base instruments. For 6- to 1536-well microplates. Includes 1 plastic tip cassette.

7210008 - External Dual Syringe Dispenser Module, Autoclavable
Adds 2 syringe drive dispensers to MultiFlo base instruments.

7210009 - External Dual Syringe Dispenser Module, Non-Autoclavable
Adds 2 syringe drive dispensers to MultiFlo base instruments.



Manifolds for Syringe Dispenser Modules (purchased separately)

7180548S - 2x8-tube Manifold, 96-/384-well Microplates
For 96- and 384-well dispensing, including half-area and PCR 96-well formats. Steel tips. 1 required for Dual Syringe Dispenser Module. Non-Autoclavable.

7180543S - 1x16-tube Manifold, 96-/384-well Microplates
For 96- and faster 384-well microplate dispensing. Steel tips. 2 required for Dual Syringe Dispenser Module.

7180552S - 1x32-tube Large Bore Manifold, 1536-well Microplates
For 1536-well microplate dispensing. Steel tips. Recommended with most liquids – those not containing surfactant. 2 required for Dual Syringe Dispenser Module.

7180553S - 1x32-tube Small Bore Manifold, 1536-well Microplates
For 1536-well microplate dispensing. Sapphire steel tips. Recommended with all liquids. 2 required for Dual Syringe Dispenser Module.

 

Note: P/N MFP and 7210010 each include any 1 plastic tip dispense cassette.

 

General
Number of Reagents 1 - 4 (model dependent)
Shaking Programmable in minutes and seconds, up to 60 minutes
Intensities - slow, medium, fast or variable
Soak Time Programmable in minutes and seconds, up to 60 minutes
Safety/Convenience/Maintenance
Adjustment utility for plate positioning
Pre-programmed maintenance routines
Automation BioStack™ Microplate Stacker - up to 50 SBS standard height plates (optional)
Onboard Software 2 x 24 character LCD display
25 alphanumeric soft keys
Create, edit or run multiple protocols
Software Liquid Handling Control™, for PC protocol programming and execution (optional)
Operating Temperature 15° C to 35° C (59° F to 95° F)
Humidity Maximum 80% relative humidity at temperatures up to 31° C (87.8° F) decreasing linearly to 50% at 40° C (104° F)
Electrical Compatible with 100 - 240 VAC ±10% 50-60 Hz
Dispensing - Peristaltic pump
Manifold Types 8-tip cassette (1 x 8) - sapphire jeweled 316 stainless steel, 316 stainless steel or polypropylene plastic tips
Labware Types 6-, 12-, 24-, 48-, 96-, 384- and 1536-well microplates in low profile, standard height and deep well formats (manifold dependent)
PCR trays
Microtubes
Maximum height: 47 or 50 mm (carrier dependent)
Dispensing Speed 10 µL /well, 96 wells, 8-tip cassette: 3 seconds
5 µL /well, 384 wells, 8-tip cassette: 6 seconds
1 µL /well, 1536 wells, 8-tip cassette: 18 seconds
500 nL /well, 1536 wells, 8-tip cassette: 15 seconds
500 nL /well, 384 wells, 8-tip cassette: 6 seconds
Volume Range 500 nL - 3,000 µL/well
Selectable in 1 µL increments
Fluid Delivery One or two positive displacement peristaltic pump
Flow Rates Low, medium or high
Cassette Size 1 µL
Recommended Volume Range: 1 - 50 µL
Dispense Accuracy: at 1 µL ±5% typical
Dispense Precision: at 1 µL <5% CV typical
Dispense Precision: at 500 nL < 10% CV typical
Minimum Prime Volume: 0.78 - 1.20 mL

5 µL
Recommended Volume Range: 5 - 2,500 µL
Dispense Accuracy: at 5 µL ±2% typical
Dispense Precision: at 5 µL <2.5% CV typical
Minimum Prime Volume: 2.75 - 4.23 mL

10 µL
Recommended Volume Range: 10 - 3,000 µL
Dispense Accuracy: at 10 µL ±2% typical
Dispense Precision: at 10 µL <2% CV typical
Minimum Prime Volume: 4.79 - 7.36 mL
Recommended Replacement Interval 1 µL Cassette: 1,000 384-well microplates at 5 µL well
5 µL Cassette: 1,000 96-well microplates at 50 µL well
10 µL Cassette: 1,000 96-well microplates at 100 µL well
Sterilization Autoclave:
Up to 50 cycles at temperatures and pressures of 121° C and 1 bar (750 mmHg)

Chemical:
70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Dispensing - Syringe pump
Minimum Prime Volume 12 mL
Manifold Types 96- / 384-well dispensing:
One 16-tube (2 x 8) manifold - 316 stainless steel tubes

96- / 384-well dispensing:
Two 16-tube (1 x 16) manifolds - 316 stainless steel tubes

1536-well dispensing:
Two 32-tube (1 x 32) manifolds - sapphire jeweled 316 stainless steel SB or 316 stainless steel LB tubes
Labware Types 96-, 384- and 1536-well microplates in low profile, standard height and deep well formats (manifold dependent)
PCR trays
Microtubes
Maximum height: 47 or 50 mm (carrier dependent)
Dispensing Speed 20 µL /well, 96 wells, 1 x16: 5 seconds
20 µL /well, 384 wells, 1 x16: 14 seconds
3 µL /well, 1536 wells, 1 x 32: 14 seconds
3 µL /well, 1536 wells, 2 x 32: 7 seconds
Volume Range 3 - 3,000 µL/well (manifold dependent)
Selectable in 1 µL increments
Fluid Delivery 2 microprocessor-controlled syringe drives
Flow Rates 1 - 5
Dispense Accuracy +1 µL typical at 5 µL
+1 µL typical at 20 µL
+1% typical at 100 µL
Dispense Precision <5% CV typical at 5 µL
<2.5% CV typical at 20 µL
<1% CV typical at 100 µL
Supply Bottle Volume 1 L or 2 L (model dependent)
Sterilization Autoclave: (model dependent)
Up to 50 cycles at temperatures and pressures of 121° C and 1 bar (750 mmHg)

Chemical:
70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Physical Characteristics
Dimensions Base instrument:
Depth: 10.6" (26.9 cm)
Width: 16.1" (40.9 cm)
Height: 7.6" (19.3 cm)
Weight Base instrument:
14.3 lbs (6.5 kg)
Regulatory
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change.

Optional

BioStack3 Microplate Stacker

BioStack3 Microplate Stacker

BioStack3 is the fastest stacker available to integrate with BioTek microplate instruments including washers, dispensers and readers. The new design allows compact, walk-away automation for benchtop or laboratory enclosure use.


Liquid Handling Control Software

Liquid Handling Control Software

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.


Optional

BioStack Integration Kits

7310044 BioStack Integration Kit for MultiFlo

Bottles & Tubing

7180541 Dispense Tubing Set - Teflon Lined
7183002 Supply Tubing Set for DMSO & Acetonitrile for 2 Syringe Pump Dispensers

Cassettes

7170007 Large Volume Replacement Tubing Set (10 µL) - molded plastic tips accessory has picture
7170008 Medium Volume Replacement Tubing Set (5 µL) - molded plastic tips accessory has picture
7170009 Low Volume Replacement Tubing Set (1 µL) - molded plastic tips accessory has picture
7170010 Large Volume (10 µL) Cassette - molded plastic tips accessory has picture
7170011 Medium Volume (5 µL) Cassette - molded plastic tips accessory has picture
7170012 Low Volume (1 µL) Cassette - molded plastic tips accessory has picture
7170013 Large Volume (10 µL) Cassette - stainless steel tips accessory has picture
7170014 Medium Volume (5 µL) Cassette - stainless steel tips accessory has picture
7170015 Low Volume (1 µL) Cassette - sapphire jeweled, stainless steel tips accessory has picture
7170016 Low Volume (1 µL) 1536-well Cassette accessory has picture
7170017 Cassette Calibration Kit accessory has picture
7170018 Low Volume (1 µL) 1536-well Cassette - molded plastic tips accessory has picture
7170020 Large volume (10 µL) Cassette Tubing Extension Kit accessory has picture
7170021 Medium volume (5 µL) Cassette Tubing Extension Kit accessory has picture
7170022 Low volume (1 µL) Cassette Tubing Extension Kit accessory has picture
7170023 Large Volume (10 µL) Cassette - large bore, molded plastic tips
7170024 Medium Volume (5 µL) Cassette - large bore, molded plastic tips

Labware Holders

7212042 Minitube Carrier - for labware up to 50 mm in height, including 1.2 mL minitubes accessory has picture

Manifolds

7180560S 2x8 Manifold

Miscellaneous

7140516 Spare Autoclavable Syringe Head with Piston
7210509 Spare Reservoir Holder and Strap
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture

Product Qualification Packages

7210512 MultiFlo Product Qualification Package

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