MicroFill Microplate Dispenser
MicroFill™ offers an economical, compact, and reliable alternative to existing microplate dispensers. Accurate and precise dispensing are guaranteed without the time-consuming recalibration, cassette replacement, and maintenance commonly associated with other dispensers available today. An autoclavable model is also available for those applications requiring a sterile fluid path.
Using BioTek's vast expertise in liquid handling, MicroFill™ incorporates a microprocessor controlled syringe pump for optimal performance. 96-, 384- and 24-well standard microplates and deep well blocks are accommodated with flexible volume ranges from 5 µL to 6,000 µL per well. MicroFill's compact footprint makes it ideally suited to fit inside laminar flow cabinets. The user-controlled dispense flow rates allow low- to high-velocity dispensing for any application.
Superior Performance in 24-, 96- and 384-well Plates

MicroFill repeatedly delivers reagents with a range in sample viscosities. Typical performance is 1.5% CV at 80 μL.
US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).
- Robot compatible carrier accommodates 96-, 384- and 24-well standard and deep well microplates
- Microprocessor-controlled syringe pump guarantees optimal precision and accuracy
- No recalibration required
- Low-maintenance design with BioTek's long history of liquid handling instruments
- User-controlled dispense flow rates for low- to high-velocity dispensing
- Up to 75 programs stored in multiple languages at the touch of a button
- Built-in maintenance routines

MicroFill’s autoclavable syringe pump is changed in seconds for guaranteed sterility and no reagent carryover.
| Model |
MicroFill™ |
|
|
Part # |
AF1000 |
AF1000A |
| 96-/384-well | — | |
| 24-, 96- and 384-well | — | |
| Autoclavable | — | |
| Organic solvents | — | |
Language versions available: English, French, German, Italian, and Spanish.
| General | |
| Microplate Types | Microplates: 24-, 96- and 384-well standard microplates, deep-well blocks and 1.2 mL test tubes, 96-well Half Area and PCR microplates Microstrips: 1x8 and 1x16 |
| Automation | Compatible with BioStack Microplate Stacker and BioStack Twister II Microplate Handler. |
| Onboard Software | Microprocessor capacity: Up to 75 custom programs stored for instant recall. Program linking: Up to 30 programs linked together for complex assays. Language support: English, French, Spanish, German and Italian |
| PC Software | Interface Software (optional) For robotic system integration |
| Dispensing - Syringe pump | |
| Dispensing Speed | 10 µL, 96-well Microplate: 4 sec 5 µL, 384-well Microplate: 7 sec |
| Volume Range | 384 wells: 5 µL to 1,500 µL per well 96 wells: 10 µL to 3,000 µL per well 24 wells: 20 µL to 6,000 µL per well |
| Fluid Delivery | Microprocessor controlled syringe pump |
| Dispense Accuracy | +1% typical at 100 µL +1 µL typical at 20 µL +1 µL typical at 5 µL |
| Dispense Precision | < 2% CV typical at 100 µL < 5% CV typical at 20 µL < 10% CV typical at 5 µL |
| Number of Channels | 16 for both standard and deep-well 96- and 384-well microplates. 8 for 96-well Half Area microplates, 96-well PCR microplates, and 24-well microplates. |
| Number of Reagents | 1 |
| Dead Volume | < 20 ml |
| Physical Characteristics | |
| Dimensions | 18" D x 15" W x 7"H (46 cm x 38 cm x 18 cm) |
| Weight | 20 lbs (8.9 kg) |
| Regulatory | |
| Regulatory | For in vitro diagnostic use. All BioTek microplate instrumentation is CE and NRTL Safety Agency marked. For details on approvals and standards compliance, please contact BioTek. |
Specifications are subject to change.
Optional

BioStack Microplate Stacker

BioStack Twister II Microplate Handler
Standard (Included) | ||
Bottles & Tubing | ||
| 7140514 | Supply Tubing for DMSO and Acetonitrile |
|
Miscellaneous | ||
| 4070517 | Aerosol Cover |
|
| 7140006 | Priming Trough Draining Set |
|
| 7140516 | Spare Autoclavable Syringe Head with Piston | |
Operators Manual | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |
Optional | ||
Bottles & Tubing | ||
| 4070515 | Reagent Bottle (2L) with Cap and Connector |
|
| 7140505 | Spare Tubing Set for AF1000 |
|
| 7140510 | Spare Tubing Set for AF1000A | |
| 7140511 | Autoclavable Reagent Bottle (1L) with Cap and Connector |
|
| 7140514 | Supply Tubing for DMSO and Acetonitrile |
|
| 7140521 | Tubing Set for AF1000 - Teflon lined | |
| 7140522 | Tubing Set for AF1000A - Teflon lined | |
Manifolds | ||
| 7140508S | Spare 16-Channel Autoclavable Manifold | |
| 7140515S | 8-Channel Manifold for 96-well Half Area and PCR Microplates |
|
| 7140518S | 8-Channel Manifold for 24-well Microplates | |
| 7140519S | Spare 16-Channel Manifold |
|
Miscellaneous | ||
| 2872304 | Spare Stylus | |
| 7140006 | Priming Trough Draining Set |
|
| 7140516 | Spare Autoclavable Syringe Head with Piston | |
Product Qualification Packages | ||
| 7140517 | MicroFill Qualification Package | |
Test Plates & Reagents | ||
| 7773001 | BioTek Solution #2 10X Concentrate Blue Test Dye (125 mL) | |
| 7773002 | BioTek Solution #1 100X Concentrate Wetting Agent (125 mL) | |


