MicroFill Microplate Dispenser
MicroFill™ offers an economical, compact, and reliable alternative to existing microplate dispensers. Accurate and precise dispensing are guaranteed without the time-consuming recalibration, cassette replacement, and maintenance commonly associated with other dispensers available today. Autoclavability is standard available for those applications requiring a sterile fluid path.
Using BioTek's vast expertise in liquid handling, MicroFill™ incorporates a microprocessor controlled syringe pump for optimal performance. 24-, 96- and 384-well standard microplates and deep well blocks are accommodated with flexible volume ranges from 5 µL to 6,000 µL per well. MicroFill's compact footprint makes it ideally suited to fit inside laminar flow cabinets. The user-controlled dispense flow rates allow low- to high-velocity dispensing for any application.
Superior Performance in 24-, 96- and 384-well Plates

MicroFill repeatedly delivers reagents with a range in sample viscosities. Typical performance is 1.5% CV at 80 μL.
US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).
- Robot compatible carrier accommodates 24-, 96- and 384-well standard and deep well microplates
- Microprocessor-controlled syringe pump guarantees optimal precision and accuracy
- No recalibration required
- Low-maintenance design with BioTek's long history of liquid handling instruments
- User-controlled dispense flow rates for low- to high-velocity dispensing
- Up to 75 programs stored in multiple languages at the touch of a button
- Built-in maintenance routines

MicroFill’s autoclavable syringe pump is changed in seconds for guaranteed sterility and no reagent carryover.
| Model |
MicroFill™ |
|
Part # |
AF1000A |
| 24-, 96- and 384-well | — |
| Autoclavable | — |
| Organic solvents | — |
Language versions available: English, French, German, Italian, and Spanish.
| General | |
| Number of Reagents | 1 |
| Microplate Types | 24-, 96- and 384-well microplates, in low profile, standard and deep well formats PCR trays Microtubes |
| Soak Time | Programmable in minutes and seconds, up to 60 minutes |
| Safety/Convenience/Maintenance | Pre-programmed maintenance routines |
| Automation | BioStack Microplate Stacker - up to 50 SBS standard height plates (optional) |
| Onboard Software | 2 x 24 character LCD display 25 alphanumeric keys Create, edit or run multiple protocols |
| Software | Interface software for robotic system integration |
| Operating Temperature | 15° C to 35° C (59° F to 95° F) |
| Humidity | Maximum 80% relative humidity at temperatures up to 31° C (87.8° F) decreasing linearly to 50% at 40° C (104° F) |
| Electrical | Compatible with 100 - 240 VAC +10% 50-60 Hz |
| Dispensing - Syringe pump | |
| Minimum Prime Volume | 10 mL |
| Manifold Types | 24-well dispensing: One 8-tube (1x8) manifold - 316 stainless steel tubes 96-well dispensing: One 8-tube (1x8) manifold - 316 stainless steel tubes 96-/384-well dispensing: One 16-tube (1x16) manifold - 316 stainless steel tubes |
| Dispensing Speed | 10 µL/well, 96 wells, 1 x 16: 4 sec 5 µL/well, 384-wells 1 x 16: 7 sec |
| Volume Range | 5 - 6000 µL/well (manifold dependent) |
| Fluid Delivery | One positive displacement syringe drive |
| Flow Rates | 1 - 5 |
| Dispense Accuracy | +1 µL typical at 5 µL +1 µL typical at 20 µL +1% typical at 100 µL |
| Dispense Precision | <5% CV typical at 5 µL <2.5% CV typical at 20 µL <1% CV typical at 100 µL |
| Number of Reagents | 1 |
| Supply Bottle Volume | 1 L |
| Sterilization | Autoclave: Up to 50 cycles at temperatures and pressures of 121° C and 1 bar (750 mmHg) Chemical: 70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution |
| Physical Characteristics | |
| Dimensions | 18" D x 15" W x 7"H (46 cm x 38 cm x 18 cm) |
| Weight | 20 lbs (8.9 kg) |
| Regulatory | |
| Regulatory | CE and TUV marked. In Vitro Diagnostic use models are available. |
Specifications are subject to change.
Optional

BioStack3 Microplate Stacker
Standard (Included) | ||
Bottles & Tubing | ||
| 7140510 | Spare Tubing Set for AF1000A | |
| 7140511 | Autoclavable Reagent Bottle (1L) with Cap and Connector |
|
Manifolds | ||
| 7140508S | Spare 16-Channel Manifold, for 96- and 384-Well Microplates | |
Miscellaneous | ||
| 2872304 | Spare Stylus | |
| 4070517 | Aerosol Cover |
|
| 7140006 | Priming Trough Draining Set |
|
Operators Manual | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |
Optional | ||
Bottles & Tubing | ||
| 7140514 | Supply Tubing for DMSO and Acetonitrile |
|
| 7140522 | Tubing Set for AF1000A - Teflon lined | |
Manifolds | ||
| 7140515S | 8-Channel Manifold, for 96-well Half Area and PCR Microplates |
|
| 7140518S | 8-Channel Manifold, for 24-well Microplates | |
Miscellaneous | ||
| 7140516 | Spare Autoclavable Syringe Head with Piston | |
| 7210515 | 3-Instrument Rack – for third party robotic system integration |
|
Product Qualification Packages | ||
| 7140517 | MicroFill Qualification Package | |
Test Plates & Reagents | ||
| 7773001 | BioTek Solution #2 10X Concentrate Blue Test Dye (125 mL) | |
| 7773002 | BioTek Solution #1 100X Concentrate Wetting Agent (125 mL) | |
Request a Quote ▶


