ELx50 Microplate Strip Washer
BioTek's years of experience in the development of both microplate washers and automated systems for EIA applications has culminated in the ELx50™ Auto Strip Washer. As a self contained and programmable instrument, the ELx50 allows for full control of precise fluidic delivery from the gentle dripping of a simple squeeze bottle to the full force of pressure delivery systems.
The ELx50 is a fast and versatile automated strip washer which can come equipped with our patented Dual-Action™ 16-channel manifold. This breakthrough design allows for independent control of the dispense and aspiration manifolds for overfill washing and overflow protection in both 96- and 384-well formats. The problem of fitting dispense and aspirate tubes into a much smaller 384-well is solved with the ELx50.
Additionally, in a 96-well format, the Dual-Action manifold provides incomparable wash performance with two sets of dispense and aspiration tubes per well. This exclusive method of two-fold aspiration guarantees low residuals. The ELx50 Auto Strip Washer is the perfect solution for low volume users of both 96- and 384-well microplates.
Now, BioTek has expanded their popular microplate washing product line with the ELx50 Filter Microplate Washer (FMW). The ELx50 FMW model, for use with filter-bottom microplates, allows a user to process important multiplex bead assays, widely used in many diverse applications. With a unique vacuum grate and bottom aspiration, bead assays are quickly, efficiently and reliably washed. The aspiration carrier can be adjusted for optimal performance with filter pore sizes ranging from 0.45 μm to 1.2 μm. Exchange the vacuum carrier for the included standard carrier, and the ELx50 FMW is ready to wash traditional ELISAs and cell-based assays.
In addition to washing multiplex bead assays, the ELx50 FMW is well-suited to work with filtration-to-waste processes such as PCR cleanup after DNA amplification to remove unwanted residues or reaction by-products with filtrate.

The ELx50 Filter Microplate Washer provides a 96-well
microplate vacuum filtration option for filter bottom plates

The Liquid Level Alert™ option allows users the convenience of continuous
monitoring for both supply and waste bottles. At the beginning and end of a
wash protocol, the liquid level is verified to ensure sufficient wash buffer remains
in the designated dispense bottle to complete a microplate wash. Sufficient
storage capacity in the waste bottle is also verified.

The ELx50/16 offers incomparable wash results via
two sets of dispense and aspiration tubes per well in
96-well format.
US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).
- Syringe drive fluid delivery system for precise control over all fluid flow rates
- Patented Dual-Action™ manifold washes both 96- and 384-well plates precisely and accurately.
- Unique priming trough built into removable microplate carrier for effortless priming and easy maintenance
- Automatic buffer switching (optional)
- Electronics completely isolated from fluidic system
- Aerosol shield
Software:
- Wash single strips or full plates
- Create, edit, delete and copy up to 75 programs from the keypad
- Link up to 10 programs to run the most complex routines
- Multi-user friendly; total programming control for complex research applications, yet easy to use for routine clinical work
- Programmable shaking duration and intensity
- Easy to use and maintain with built-in maintenance programs
- Bottom washing and crosswise aspiration protocols
- Language versions (optional): English, French, German, Italian, and Spanish
| Model | Part # | 96-well only | 96-/384-well | Valve option | Filter Plate option |
| ELx50™ | ELX50/8 | • |
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| ELX50/8V | • |
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| ELX50/12 | • |
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| ELX50/12V | • |
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| ELX50/16 | • |
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| ELX50/16V | • |
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| ELX50/8FMW | • |
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| General | |
| Microplate Types | All flat-bottom 96-well plates, 384- or 96-well flat-bottom plates, 1x8 flat-bottom strips (16-channel) All 96-well plates, 1x8 strips (8-channel) All 96-well plates, 1x12 strips (12-channel) Filter Microplate Washer - Filter Plate Washing: 96-well plates Filter pore sizes from 0.45 µM - 1.2 µM Filter Microplate Washer - Solid Bottom Plate Washing: All 96-well plates, 1x8 strips |
| Shaking | User-programmable speeds and timing |
| Soak Time | 1 - 600 seconds |
| Onboard Software | Create up to 75 customized protocols including Prime, Aspirate, Soak and Dispense Program Linking - Link up to 10 previously created protocols Soak times of 1 - 600 seconds Selectable shake speed and duration Dispense volumes of 25 - 3,000 µL per well Variable fluid flow rates of 150 - 1,000 µL per well per sec AutoPrime program Overfill wash and overflow protection Up to 10 cycles per Wash protocol Bottom washing Crosswise aspiration Filter Microplate Washer -Filter Plate Washing: Bottom aspiration times of 1 - 180 seconds |
| Washing | |
| Manifold Type | 16-channel patented Dual-Action, with separate dispense/aspiration (16-well) 8-channel aspiration/dispense (8-channel) 12-channel aspiration/dispense (12-channel) |
| Washing Speed | 3 asp. / disp. cycles: <250 seconds (100 µL/well, 384 wells) <105 seconds (300 µL/well, 96 wells) Filter Microplate Washer - Filter Plate Washing: Variable, based on wash parameters |
| Vacuum Filtration | Vacuum chamber integrated into microplate carrier Selectable vacuum levels:
Integrated priming trough for unattended startup Hold down clamp for guaranteed seal |
| Volume Range | 25 - 3,000 µL/well |
| Buffer Selection | Optional valve module for up to 3 buffers |
| Wash Cycles | 1-10 |
| Dispense Precision | <3% CV |
| Residual Volume | <2 µL/well Filter Microplate Washer - Filter Plate Washing: No apparent residual inside wells after a 3-cycle wash, dispensing 250 µL per well of deionized water with 0.1% Tween 20 and aspiration times of 30 seconds Minimal liquid droplets on underside of plate after a 3-cycle wash |
| Sterilization | Chemical |
| Supply Bottle Volume | 2 liter |
| Physical Characteristics | |
| Dimensions | 16"D x 14"W x 6.5"H (40.6 x 35.6 x 16.5 cm) |
| Weight | 20 lbs (8.9 kg) |
| Regulatory | |
| Regulatory | IVD (In Vitro Diagnostics) Directive. All BioTek microplate instrumentation is CE and NRTL Safety Agency marked. For details on approvals and standards compliance, please contact BioTek. |
Specifications are subject to change.
Standard (Included) | ||
Bottles & Tubing | ||
| 4070510 | Supply Tubing Set |
|
| 4070515 | Reagent Bottle (2L) with Cap and Connector |
|
Dispense/Waste Systems | ||
| 48140 | 2-Liter Waste Bottle (without cap) |
|
Manifolds | ||
| 4070512S | 8-Channel Manifold |
|
| 4070513S | 12-Channel Manifold |
|
| 7140519S | Spare 16-Channel Manifold |
|
Miscellaneous | ||
| 4070517 | Aerosol Cover |
|
Operators Manual | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |
Optional | ||
Bottles & Tubing | ||
| 4070510 | Supply Tubing Set |
|
| 4070511 | Waste Tubing Set |
|
| 4070515 | Reagent Bottle (2L) with Cap and Connector |
|
Dispense/Waste Systems | ||
| 48140 | 2-Liter Waste Bottle (without cap) |
|
Manifolds | ||
| 4070512S | 8-Channel Manifold |
|
| 4070513S | 12-Channel Manifold |
|
| 4070519S | 8-Channel Manifold with short dispense tubes |
|
Miscellaneous | ||
| 4070035 | Liquid Level Alert Kit for non-valve option ELx50 models | |
| 4070036 | Liquid Level Alert Kit for valve option ELx50 models |
|
| 4070517 | Aerosol Cover |
|
| 4070523 | Stylus Kit |
|
Product Qualification Packages | ||
| 4070530 | ELx50 Qualification Package | |
Test Plates & Reagents | ||
| 7773001 | BioTek Solution #2 10X Concentrate Blue Test Dye (125 mL) | |
| 7773002 | BioTek Solution #1 100X Concentrate Wetting Agent (125 mL) | |
Vacuum Systems | ||
| 48146 | Vacuum Line Filter | |
| 4073009 | Silencing muffler | |



