BioTek: Microplate Instrumentation & Software Solutions
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ELx50 Microplate Strip Washer

BioTek's years of experience in the development of both microplate washers and automated systems for EIA applications has culminated in the ELx50™ Auto Strip Washer. As a self contained and programmable instrument, the ELx50 allows for full control of precise fluidic delivery from the gentle dripping of a simple squeeze bottle to the full force of pressure delivery systems.

The ELx50 is a fast and versatile automated strip washer which can come equipped with our patented Dual-Action™ 16-channel manifold. This breakthrough design allows for independent control of the dispense and aspiration manifolds for overfill washing and overflow protection in both 96- and 384-well formats. The problem of fitting dispense and aspirate tubes into a much smaller 384-well is solved with the ELx50.

Additionally, in a 96-well format, the Dual-Action manifold provides incomparable wash performance with two sets of dispense and aspiration tubes per well. This exclusive method of two-fold aspiration guarantees low residuals. The ELx50 Auto Strip Washer is the perfect solution for low volume users of both 96- and 384-well microplates.

Now, BioTek has expanded their popular microplate washing product line with the ELx50 Filter Microplate Washer (FMW). The ELx50 FMW model, for use with filter-bottom microplates, allows a user to process important multiplex bead assays, widely used in many diverse applications. With a unique vacuum grate and bottom aspiration, bead assays are quickly, efficiently and reliably washed. The aspiration carrier can be adjusted for optimal performance with filter pore sizes ranging from 0.45 μm to 1.2 μm. Exchange the vacuum carrier for the included standard carrier, and the ELx50 FMW is ready to wash traditional ELISAs and cell-based assays.

In addition to washing multiplex bead assays, the ELx50 FMW is well-suited to work with filtration-to-waste processes such as PCR cleanup after DNA amplification to remove unwanted residues or reaction by-products with filtrate.




The ELx50 Filter Microplate Washer provides a 96-well
microplate vacuum filtration option for filter bottom plates





The Liquid Level Alert™ option allows users the convenience of continuous
monitoring for both supply and waste bottles. At the beginning and end of a
wash protocol, the liquid level is verified to ensure sufficient wash buffer remains
in the designated dispense bottle to complete a microplate wash. Sufficient
storage capacity in the waste bottle is also verified.





The ELx50/16 offers incomparable wash results via
two sets of dispense and aspiration tubes per well in
96-well format.

 

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).

Hardware:
  • Syringe drive fluid delivery system for precise control over all fluid flow rates
  • Patented Dual-Action™ manifold washes both 96- and 384-well plates precisely and accurately.
  • Unique priming trough built into removable microplate carrier for effortless priming and easy maintenance
  • Automatic buffer switching (optional)
  • Electronics completely isolated from fluidic system
  • Aerosol shield

Software:
  • Wash single strips or full plates
  • Create, edit, delete and copy up to 75 programs from the keypad
  • Link up to 10 programs to run the most complex routines
  • Multi-user friendly; total programming control for complex research applications, yet easy to use for routine clinical work
  • Programmable shaking duration and intensity
  • Easy to use and maintain with built-in maintenance programs
  • Bottom washing and crosswise aspiration protocols
  • Language versions (optional): English, French, German, Italian, and Spanish
Model Part # 96-well only 96-/384-well Valve option Filter Plate option
ELx50™   ELX50/8
     
ELX50/8V
 
 
ELX50/12
      
ELX50/12V
 
 
ELX50/16  
    
ELX50/16V  
 
ELX50/8FMW
   

 

General
Microplate Types All flat-bottom 96-well plates, 384- or 96-well flat-bottom plates, 1x8 flat-bottom strips (16-channel)
All 96-well plates, 1x8 strips (8-channel)
All 96-well plates, 1x12 strips (12-channel)

Filter Microplate Washer - Filter Plate Washing:
96-well plates
Filter pore sizes from 0.45 µM - 1.2 µM

Filter Microplate Washer - Solid Bottom Plate Washing:
All 96-well plates, 1x8 strips
Shaking User-programmable speeds and timing
Soak Time 1 - 600 seconds
Onboard Software Create up to 75 customized protocols including Prime, Aspirate, Soak and Dispense
Program Linking - Link up to 10 previously created protocols
Soak times of 1 - 600 seconds
Selectable shake speed and duration
Dispense volumes of 25 - 3,000 µL per well
Variable fluid flow rates of 150 - 1,000 µL per well per sec
AutoPrime program
Overfill wash and overflow protection
Up to 10 cycles per Wash protocol
Bottom washing
Crosswise aspiration

Filter Microplate Washer -Filter Plate Washing:
Bottom aspiration times of 1 - 180 seconds
Washing
Manifold Type 16-channel patented Dual-Action, with separate dispense/aspiration (16-well)
8-channel aspiration/dispense (8-channel)
12-channel aspiration/dispense (12-channel)
Washing Speed 3 asp. / disp. cycles:
<250 seconds (100 µL/well, 384 wells)
<105 seconds (300 µL/well, 96 wells)

Filter Microplate Washer - Filter Plate Washing:
Variable, based on wash parameters
Vacuum Filtration Vacuum chamber integrated into microplate carrier
Selectable vacuum levels:
  • Low vacuum -64 mmHg, 0.047" hole
  • Medium vacuum -116 mmHg, 0.032" hole vent plug
  • High vacuum -240 mmHg, 0.020" hole vent plug
Droplet director for minimized plate bottom residual and no cross contamination
Integrated priming trough for unattended startup
Hold down clamp for guaranteed seal
Volume Range 25 - 3,000 µL/well
Buffer Selection Optional valve module for up to 3 buffers
Wash Cycles 1-10
Dispense Precision <3% CV
Residual Volume <2 µL/well

Filter Microplate Washer - Filter Plate Washing:
No apparent residual inside wells after a 3-cycle wash, dispensing 250 µL per well of deionized water with 0.1% Tween 20 and aspiration times of 30 seconds
Minimal liquid droplets on underside of plate after a 3-cycle wash
Sterilization Chemical
Supply Bottle Volume 2 liter
Physical Characteristics
Dimensions 16"D x 14"W x 6.5"H (40.6 x 35.6 x 16.5 cm)
Weight 20 lbs (8.9 kg)
Regulatory
Regulatory IVD (In Vitro Diagnostics) Directive. All BioTek microplate instrumentation is CE and NRTL Safety Agency marked. For details on approvals and standards compliance, please contact BioTek.

Specifications are subject to change.

Standard (Included)

Bottles & Tubing

4070510 Supply Tubing Set accessory has picture
4070515 Reagent Bottle (2L) with Cap and Connector accessory has picture

Dispense/Waste Systems

48140 2-Liter Waste Bottle (without cap) accessory has picture

Manifolds

4070512S 8-Channel Manifold accessory has picture
4070513S 12-Channel Manifold accessory has picture
7140519S Spare 16-Channel Manifold accessory has picture

Miscellaneous

4070517 Aerosol Cover accessory has picture

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Optional

Bottles & Tubing

4070510 Supply Tubing Set accessory has picture
4070511 Waste Tubing Set accessory has picture
4070515 Reagent Bottle (2L) with Cap and Connector accessory has picture

Dispense/Waste Systems

48140 2-Liter Waste Bottle (without cap) accessory has picture

Manifolds

4070512S 8-Channel Manifold accessory has picture
4070513S 12-Channel Manifold accessory has picture
4070519S 8-Channel Manifold with short dispense tubes accessory has picture

Miscellaneous

4070035 Liquid Level Alert Kit for non-valve option ELx50 models
4070036 Liquid Level Alert Kit for valve option ELx50 models accessory has picture
4070517 Aerosol Cover accessory has picture
4070523 Stylus Kit accessory has picture

Product Qualification Packages

4070530 ELx50 Qualification Package

Test Plates & Reagents

7773001 BioTek Solution #2 10X Concentrate Blue Test Dye (125 mL)
7773002 BioTek Solution #1 100X Concentrate Wetting Agent (125 mL)

Vacuum Systems

48146 Vacuum Line Filter

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Certifications

  • IVD Compliant

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