BioTek: Microplate Instrumentation & Software Solutions
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ELx50 Microplate Strip Washer

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Automated strip washer for
96- and 384-well plates



Vacuum filtration and magnetic bead assays

BioTek's years of experience in the development of both microplate washers and automated systems for EIA applications has culminated in the ELx50™ Strip Washer. As a self contained and programmable instrument, the ELx50 allows for full control of precise fluidic delivery from the gentle dripping of a simple squeeze bottle to the full force of pressure delivery systems.

The new ELx50 Washer is now a truly flexible platform that provides washing capabilities unsurpassed in its class. Newly available models with biomagnetic separation and vacuum filtration capabilities make the ELx50 an excellent choice for automating the wash steps of 96-well magnetic or polystyrene bead assays, such as those developed on the Luminex® xMAP® technology platform. The ELx50 Washer is Luminex® xMAP® approved.Users not requiring an integrated stacker can expect the same great bead washing performance as BioTek’s industry leading full plate washers but here in a more compact footprint.  Comprehensive onboard software makes programming quick and easy. Custom magnets, in either flat or ring designs, incorporate high-energy neodymium iron boron magnets for rapid separation of micrometer and nanometer magnetic beads with superior retention. A fast and efficient vacuum filtration module allows vacuum to be adjusted with a range of settings for flexibility with polystyrene beads when using various filter pore sizes and sample viscosities. The vacuum filtration module is also well suited for filtration-to-waste processes such as PCR cleanup after DNA amplification to remove unwanted residues or reaction by-products with filtrate.  The washer’s modular platform allows for processing standard solid bottom plates used in a laboratory’s many traditional ELISAs and cell based assays.

The ELx50 can also be equipped with our patented Dual-Action™ 16-channel manifold. This breakthrough design allows for independent control of the dispense and aspiration manifolds for overfill washing and overflow protection in both 96- and 384-well formats. The problem of fitting dispense and aspirate tubes into a much smaller 384-well is solved with the ELx50. Additionally, in a 96-well format, the Dual-Action manifold provides incomparable wash performance with two sets of dispense and aspiration tubes per well. This exclusive method of two-fold aspiration guarantees low residuals.

As a welcome upgrade from manual processing, the new ELx50 Microplate Strip Washer will bring to your lab an all inclusive wash solution offering consistent performance and unattended operation.

High Strength Biomagnetic Separation

Fast and Efficient Vacuum Filtration

The ELx50's custom magnet designs incorporate high-energy neodymium iron boron magnets for rapid separation of micrometer and nanometer beads with superior retention.

An available vacuum filtration module automates the washing of 96-well filter bottom plates. Vacuum is adjusted via a range of settings for optimal performance with various filter pore sizes.

 Automated Liquid Level Sensing 

 



The Liquid Level Alert™ option allows users the convenience of continuous monitoring for both supply and waste bottles. At the beginning and end of a wash protocol, the liquid level is verified to ensure sufficient wash buffer remains in the designated dispense bottle to complete a microplate wash. Sufficient storage capacity in the waste bottle is also verified.

Patented Dual-Action™ Manifold 

 

The ELx50/16 offers incomparable wash results for 96- and 384-well microplates. In 96-well format, the unique design incorporates two sets of dispense and aspiration tubes per well.

 

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).

 

xMAP® is a registered trademark of Luminex® Corporation.

General

  • Syringe drive fluid delivery system for precise control over all fluid flow rates
  • Wash single strips or full plates
  • 96- and 384-well microplates
  • Unique priming trough built into removable microplate carrier for effortless priming and easy maintenance
  • Automatic buffer switching
  • Create, edit, delete and copy up to 75 programs from the keypad
  • Link up to 10 programs to run the most complex routines
  • Multi-user friendly; total programming control for complex research applications, yet easy to use for routine clinical work
  • Programmable shaking duration and intensity
  • Built-in maintenance programs
  • Bottom washing and crosswise aspiration protocols
  • Small footprint with integrated dispense and waste pumps
  • Aerosol cover for safety

Biomagnetic Separation

  • High strength biomagnetic separation for super paramagnetic bead based assays, such as Luminex xMAP®
  • High energy neodymium iron boron magnets
  • Optimized field gradients within wells
  • Choice of flat or ring magnets - two 96-well high strength designs custom for BioTek microplate washers
  • Rapid separation of micrometer and nanometer beads
  • Superior microsphere retention

Vacuum Filtration

  • Fast and efficient vacuum filtration for polystyrene bead assays or filtration-to-waste processes
  • Range in accommodated filter pore sizes from 0.45 mm to 1.2 µmm
  • Selectable vacuum levels from –91 mmHg to –313 mmHg for varying sample viscosities
  • Carrier vacuum grate directs liquid droplets away from plate for minimized after-wash residual on plate’s underside and no cross contamination
  • Software programmable dispense volume, soak and aspiration time for completely unattended operation and recall
Model Part # 96-well only 96-/384-well Buffer Switching Biomagnetic Separation Vacuum Filtration
ELx50™   ELX508
       
ELX508M
   
 
ELX508MF
   
ELX508F
     
ELX508V
 
   
ELX5012
        
ELX5012V
 
   
ELX5016  
      
ELX5016V  
   

 

General
Microplate Types 96- and 384-well (model dependent)
Standard height and low profile
Solid and filter bottom (model dependent)
  • Filter pore sizes 0.45 µM to 1.2 µM
Assays Magnetic bead, polystyrene bead (model dependent)
  • Multiplex assays
  • Bead-based ELISA
ELISA
Cell-based assays
Protein arrays
Filtration-to-waste processes (model dependent)
Separation Biomagnetic separation, vacuum filtration (model dependent)
Shaking Programmable in minutes and seconds up to 60 minutes
Intensities - slow, medium, fast or variable
Soak Time Programmable in minutes and seconds up to 60 minutes
Magnet High strength 96-well format
Choice of two designs, custom for BioTek microplate washers:
Flat
- Flat-bottom well - beads pulled to band across well bottom
- Round-bottom well - beads pulled to button at well bottom
Ring - beads pulled to 4-zone ring at well bottom

Incorporated into carrier, removable for non-magnetic washing
Safety/Convenience/Maintenance Waste sensing, fluid detection (optional)
Aerosol cover
Overflow protection
Pre-programmed maintenance routines
Onboard Software 2 x 24 character LCD display
25 alphanumeric soft keys
Create, edit or run multiple protocols
Operating Temperature 15°C to 40°C (59° to 104° F)
Humidity 10% to 85%, non-condensing
Electrical Compatible with 100 to 240 V~ ± 10% @ 50-60 Hz
Washing
Manifold Types 96-well washing:
8-tube (1x8) manifold
12-tube (1x12) manifold 96-/384-well washing:
Dual-Action™ 16-tube (1x16) manifold

Washing Speed Solid bottom plates
3 asp./disp. cycles: >300 µL/well, 96 wells, 8-tube manifold, final aspirate: < 130 seconds

Filter bottom plates:
Variable, based on wash parameters
Fluid Delivery One positive displacement syringe drive
Vacuum Filtration Selectable vacuum levels (final at 30 seconds):

0.45 µm 96-well plates:
Low: -91 mmHg
Medium: -150 mmHg
High: -313 mmHg

1.2 µm 96-well plates:
Low: -95 mmHg
Medium: -155 mmHg
High: -299 mmHg

Vacuum filtration time range: 1 to 180 seconds
Volume Range 25 - 3,000 µL/well (model dependent)
Selectable in 1 µL increments
Buffer Selection Automatic switching for up to 3 wash buffers (model dependent)
Flow Rates 1 - 9
Wash Cycles 1 - 10
Dispense Precision ≤3% CV
Residual Volume Solid bottom plates
≤2 µL/well

Filter bottom plates
Average increase weight of plate ≤1.2 g after dispensing 300 µL to 0.45 µm plate, filtration 30 seconds, low vacuum, blotted
Sterilization Chemical:
70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Supply Bottle Volume 2 L
Physical Characteristics
Dimensions 14"W x 16"D x 6.5"H (35.6 x 40.6 x 16.5 cm)
Weight 22 lbs (9.8 kg)
Regulatory
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change.

Standard (Included)

Bottles & Tubing

48140 Waste Bottle (2L) without cap accessory has picture
4070510 Supply Tubing Set for non-buffer switching ELx50 Models accessory has picture
4070515 Dispense Bottle (2L) with Cap and Connector accessory has picture

Manifolds

4070512S 8-Channel Manifold accessory has picture
4070513S 12-Channel Manifold accessory has picture

Miscellaneous

4070517 Aerosol Cover accessory has picture

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Optional

Bottles & Tubing

48140 Waste Bottle (2L) without cap accessory has picture
4070075 Waste Bottle, 4L & Tubing
4070076 Dispense Bottle, 4L with Filter & Tubing
4070511 Waste Tubing Set for non-vacuum filtration ELx50 models accessory has picture

Dispense/Waste Systems

48146 Vacuum Line Filter
4070077 Complete Dispense/Waste System, 4L Bottles
4070078 Complete Dispense/Waste System, 4L Bottles
4073009 Silencing muffler accessory has picture

Manifolds

4070512S 8-Channel Manifold accessory has picture
4070513S 12-Channel Manifold accessory has picture
4070519S 8-Channel Manifold with short dispense tubes accessory has picture

Miscellaneous

4070035 Liquid Level Alert Kit for non-buffer switching ELx50 models
4070036 Liquid Level Alert Kit for buffer switching ELx50 models accessory has picture
4070517 Aerosol Cover accessory has picture
4070523 Stylus Kit accessory has picture
7102216 96-well Ring Magnet accessory has picture
7103016 96-well Flat Magnet accessory has picture

Product Qualification Packages

4070530 ELx50 Qualification Package
4070566 ELx50 Qualification Package - for 8M, 8F and 8MF models

Test Plates & Reagents

7773001 BioTek Solution #2 10X Concentrate Blue Test Dye (125 mL)
7773002 BioTek Solution #1 100X Concentrate Wetting Agent (125 mL)

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