ELx405 Select Deep Well Microplate Washer

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Washes 96- and 384-well deep well and standard height plates

Ideal for cell culture, plasmid DNA purification and much more

From the leaders in microplate washing, BioTek introduces the world’s first deep well microplate washer that aspirates and dispenses into 96- and 384-well plates <50 mm in height. ANSI/SBS standard microplates are also accommodated, resulting in a total solution for all of a laboratory’s solid bottom microplate washing needs. The new ELx405™ Select Deep Well Microplate Washer is a robot compatible, full plate washer incorporating BioTek’s patented Dual-Action™ manifold with independent filling and evacuation control for precise overfill washing and overflow protection. Available low-flow rates and angled dispensing make the ELx405 particularly useful in cell-based assays.

Scientists regard unexpected clogging of manifold tubes to be the largest contributor to assay failure. Cleaning these tubes is a time consuming and labor intensive process.  With optional Ultrasonic Advantage™, BioTek washers are the only products available today with built in ultrasonic cleaners able to automatically prime with cleaning solution, sonicate tubes to remove any protein or salt crystal build up and re-prime with rinse or wash buffer leaving the instrument clean and ready for its next wash.

BioTek's Liquid Handling Control Software provides full PC control of the ELx405 Select Deep Well in a familiar Microsoft Windows® environment, while its intuitive StepWise™ protocol creation makes LHC Software easy to use even for novice users.  LHC Secure offers 21 CFR Part 11 compliance with flexible multi-user permission levels and electronic protocol and system audit trail signing additional security is required.


Deep Well Plates
• Plasmid DNA purification
• Serum/plasma sample preparation
• Cell signaling – phospho flow setup for flow cytometry
• Hematopoietic progenitor cell enumeration
• Cell culture – bacterial, yeast and mammalian cells grown in suspension

Standard Height Plates
• MSD assays
• HCS immune cytochemistry
• FLIPR® Ca2+ flux
• Cell-based assays
• Magnetic bead assays – such as gene expression and cytokine
• ELISPOT assays


US Federal Government customers:This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).


xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®


  • Washes microplates <50 mm in height
  • Accommodates 96- and 384-well formats
  • Automation of dispense and aspiration steps of conventional ELISA workflows
  • Optional cell washing with gentle, low-flow rates and 20° angled dispense tubes
  • Patented Dual-Action™ manifold for independent control of the aspirate and dispense manifolds for precise overfill washing and overflow protection
  • Internal positive displacement pump eliminates the use of troublesome pressure delivery systems and allows the use of any size dispense reservoir
  • Optional external valve module connects four reagent bottles with automatic switching between reagents
  • Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates the need for blank plates when used in automated robotic systems
  • Optional Ultrasonic Advantage™ – built in ultrasonic cleaner formanifold self-maintenance
  • Built-in fluid flow, fluid detection, and vacuum sensing provide complete protection for unattended operation
  • Biomagnetic separation ready with ANSI/SBS standard height microplates
  • BioStack compatible for unattended processing of up to 50 ANSI/SBS standard height microplates


Patented Dual-Action™ manifold design for 96- and 384-well plates.

The ELx405™ Select Deep Well Washer aspirates and dispenses into full size 96- and 384-well blocks with unattended operation.



  • 75 programs
  • Link up to 10 programs (if valve module is attached, links multiple buffers automatically)
  • Pre/AutoPrime
  • 10 cycles/wash
  • Crosswise aspiration
  • Fully programmable for total control of washing cycles from gentle cell-washing to vigorous wash for high-binding assays
    • Dispense height
    • Aspiration height
    • Aspiration delay
    • Soak
    • Shake
    • Aspiration rate
    • Horizontal aspirate and dispense position
  • External programming/downloading available through USB or RS-232 ports
  • Software control from onboard keypad or Liquid Handling Control™ PC Software
  • 21 CFR Part 11 compliance with LHC™ Secure
Part #
96-/384- well
Deep Well
Standard Height
Cell Washing
Buffer Switching
Biomagnetic Separation*


* With available module sold separately. Biomagnetic separation compatible with standard height plates only.


ELx405™ Select Deep Well Microplate Washer Configurations
  Feature Design
S Ultrasonic Advantage™
  • Built-in ultrasonic cleaner for manifold self-maintenace
V Automatic 4-Buffer Switching  
U 96-/384-well microplate washing
  • 96-tube (8 x 12) manifold
  • Dual-Action™
D Deep well microplates
  • Accomodates ANSI/SBS standard and deep well microplates <50 mm in height
CW Optimized cell washing for loosely adherent monolayers  


Microplate Types 96- and 384-well
Low profile and standard height
Deep well up to 50 mm
Assays ELISA
Cell-based assays (configuration dependent)
Magnetic bead, polystyrene bead (configuration dependent; ; Biomagnetic separation compatible with standard height plates only )
  • Multiplex assays
  • Bead-based ELISA

Fluid Delivery Internal positive displacement pump
Shaking Programmable in minutes and seconds, up to 60 minutes
Intensities – slow, medium, fast or variable
Soak Time Programmable in minutes and seconds, up to 60 minutes
Safety/Convenience/Maintenance Waste and vacuum sensing, fluid and flow detection
Aerosol cover
Adjustment utility for plate positioning
Overflow protection
Pre-programmed maintenance routines
Automation Compatible with BioStack (excluding deep well plates) and 3rd party automation
Onboard Software 2 x 24 character backlit LCD display
25 alphanumeric soft keys
Create, edit or run multiple protocols
Software Liquid Handling Control™, for PC wash protocol programming and execution (optional)
Operating Temperature 15°C to 30°C (59°F to 86°F)
Interface USB and 9-pin RS232
Humidity 10% to 85%, non-condensing
Electrical 100 - 240 Volts AC 50/60 Hz, <8.0 A
Manifold Types Dual-Action 96-tube (8 x 12) manifold
Washing Speed 96-well:
< 30 seconds: 3 aspirate / dispense cycles, 300 µL/well, no soak, final aspirate
< 80 seconds: 3 aspirate / dispense cycles, 100 µL/well, no soak, final aspirate
Volume Range 50 - 3000 µL/well
Selectable in 1 µL increments
Buffer Selection Automatic switching for up to 4 wash buffers (configuration dependent)
Flow Rates 1 - 11
Includes low flow, cell wash rates (model dependent)
Wash Cycles 1 - 10
Dispense Precision ≤3% CV: 300 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate of 300µL/well/second, Corning 96-well flat-bottom plate, absorbance read at 630 nm and 450 nm reference
Residual Volume Standard 96-well plate
<2 µL/well: 3-cycle wash, 300 µL/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height optimized prior to testing
Deep 96-well plate
<2 µL/well: 3-cycle wash, 2,000 µL/well (deionized water with 0.1% Tween 20), Corning 96-well storage block - P/N 3960 or 3961
Sterilization Chemical
Ultrasonic Cleaning Built-in ultrasonic bath for protein and salt crystal buildup
Programmable in hours and minutes, up to 60 minutes (optional)
Supply Bottle Volume 4 L or 10 L (optional)
Physical Characteristics
Dimensions Depth: 17" (43.2 cm)
Width: 14" (35.6 cm)
Height: 11" (27.9 cm)
Weight 30 lbs (13.5 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.


BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.

Liquid Handling Control™ Software

Liquid Handling Control™ Software

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.

Standard (Included)

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center


Bottles & Tubing

7100531 Cap for Waste Bottle, 4L (Also fits 10L or 20L) accessory has picture
7100532 Spare Cap for 4L Dispense Bottle accessory has picture
7100533 Waste Tubing Set accessory has picture
7100534 Waste Bottle, 4L accessory has picture
7100535 Spare 4L Dispense Bottle Assembly accessory has picture
7100538 Dispense Tubing Set –1 Buffer accessory has picture
7100541S Dispense System Complete Assembly, 4L
7100542 Waste Bottle with Level Sensing, 4L accessory has picture
7100543S Waste Set with Level Sensing, 4L Bottles accessory has picture
7100544 Cap for Waste Bottle with Level Sensing, 4L accessory has picture
7100556 Waste Bottle and Tubing, 20L accessory has picture
7100557 Waste Bottle and Tubing, 10L accessory has picture
7100559 Dispense Bottle (10L) accessory has picture

Dispense/Waste Systems

7100581S Dispense System Complete Assembly, 10L accessory has picture
7100582S Vacuum Set with Level Sense Assembly, 10L accessory has picture
7100745S Complete Dispense/Waste System 115/230V, 4L Bottles accessory has picture
7100746S Complete Waste System 115V/230V, 4L Bottles accessory has picture
7100751 Complete Dispense/High Flow Waste System 115V/230V, 4L Bottles accessory has picture
7100752 Complete Dispense/High Flow Waste System 115/230V, 10L Bottles accessory has picture
7100753S Complete High Flow Waste System 115V/230V, 4L Bottles
7100754 High Flow Vacuum Pump 115/230V
7103024 Standard Vacuum Pump 115V/230V accessory has picture


49943 Dispense Fluid Inlet Screen
48294 Filter for Waste Tubing accessory has picture


01113 Silencing Muffler for Standard Vacuum Pump
4030551 Vacuum Gauge/Regulator
7100025S Stylus Kit for any 405™/ELx405™ Select Deep Well manifold accessory has picture
7102215 384-well Ring Magnet accessory has picture
7102216 96-well Ring Magnet accessory has picture
7103016 96-well Flat Magnet accessory has picture
7103017 384-well Flat Magnet accessory has picture
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture

Product Qualification Packages

7100567 ELx405 Qualification Package

Test Plates & Reagents

7773001 BioTek Solution #2 10X Concentrate Blue Test Dye (125 mL)
7773002 BioTek Solution #1 100X Concentrate Wetting Agent (125 mL)

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