ELx405 Select Deep Well Washer

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Washes 96- and 384-well deep well and standard height plates

Washes 96- and 384-well deep well and standard height plates

Ideal for cell culture, plasmid DNA purification and much more

Ideal for cell culture, plasmid DNA purification and much more

From the leaders in microplate washing, BioTek introduces the world’s first deep well microplate washer that aspirates and dispenses into 96- and 384-well plates <50 mm in height. ANSI/SBS standard microplates are also accommodated, resulting in a total solution for all of a laboratory’s solid bottom microplate washing needs. The new ELx405™ Select Deep Well Microplate Washer is a robot compatible, full plate washer incorporating BioTek’s patented Dual-Action™ manifold with independent filling and evacuation control for precise overfill washing and overflow protection. Available low-flow rates and angled dispensing make the ELx405 particularly useful in cell-based assays.

Scientists regard unexpected clogging of manifold tubes to be the largest contributor to assay failure. Cleaning these tubes is a time consuming and labor intensive process.  With optional patented Ultrasonic Advantage™, BioTek washers are the only products available today with built in ultrasonic cleaners able to automatically prime with cleaning solution, sonicate tubes to remove any protein or salt crystal build up and re-prime with rinse or wash buffer leaving the instrument clean and ready for its next wash.

BioTek's Liquid Handling Control Software provides full PC control of the ELx405 Select Deep Well in a familiar Microsoft Windows® environment, while its intuitive StepWise™ protocol creation makes LHC Software easy to use even for novice users.  LHC Secure offers 21 CFR Part 11 compliance with flexible multi-user permission levels and electronic protocol and system audit trail signing additional security is required.

Applications:

Deep Well Plates
• Plasmid DNA purification
• Serum/plasma sample preparation
• Cell signaling - phospho flow setup for flow cytometry
• Hematopoietic progenitor cell enumeration
• Cell culture - bacterial, yeast and mammalian cells grown in suspension

Standard Height Plates
• ELISAs
• MSD assays
• HCS immune cytochemistry
• FLIPR® Ca2+ flux
• Cell-based assays
• Magnetic bead assays – such as gene expression and cytokine
panels
• ELISPOT assays

 

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

 

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®

Hardware:

  • Washes microplates <50 mm in height
  • Accommodates 96- and 384-well formats
  • Automation of dispense and aspiration steps of conventional ELISA workflows
  • Optional cell washing with gentle, low-flow rates and 20° angled dispense tubes
  • Patented Dual-Action™ manifold for independent control of the aspirate and dispense manifolds for precise overfill washing and overflow protection
  • Internal positive displacement pump eliminates the use of troublesome pressure delivery systems and allows the use of any size dispense reservoir
  • Optional external valve module connects four reagent bottles with automatic switching between reagents
  • Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates the need for blank plates when used in automated robotic systems
  • Optional patented Ultrasonic Advantage™ – built in ultrasonic cleaner formanifold self-maintenance
  • Built-in fluid flow, fluid detection, and vacuum sensing provide complete protection for unattended operation
  • Biomagnetic separation ready with ANSI/SBS standard height microplates
  • BioStack compatible for unattended processing of up to 50 ANSI/SBS standard height microplates

 



Patented Dual-Action™ manifold design for 96- and 384-well plates.

The ELx405™ Select Deep Well Washer aspirates and dispenses into full size 96- and 384-well blocks with unattended operation.

 

Software:

  • 75 programs
  • Link up to 10 programs (if valve module is attached, links multiple buffers automatically)
  • Pre/AutoPrime
  • 10 cycles/wash
  • Crosswise aspiration
  • Fully programmable for total control of washing cycles from gentle cell-washing to vigorous wash for high-binding assays
    • Dispense height
    • Aspiration height
    • Aspiration delay
    • Soak
    • Shake
    • Aspiration rate
    • Horizontal aspirate and dispense position
  • External programming/downloading available through USB or RS-232 ports
  • Software control from onboard keypad or Liquid Handling Control™ PC Software
  • 21 CFR Part 11 compliance with LHC™ Secure
Part #
96-/384- well
Deep Well
Standard Height
Cell Washing
Buffer Switching
Ultrasonic
Biomagnetic Separation*
ELX405UD
 
 
 
ELX405USD
 
 
ELX405UVD
 
 
ELX405UVSD
 
ELX405UCWD
 
 
ELX405UCWSD
 
ELX405UCWVD
 
ELX405UCWVSD

 

* With available module sold separately. Biomagnetic separation compatible with standard height plates only.

 

ELx405™ Select Deep Well Microplate Washer Configurations
  Feature Design
S Ultrasonic Advantage™
  • Built-in ultrasonic cleaner for manifold self-maintenace
V Automatic 4-Buffer Switching  
U 96-/384-well microplate washing
  • 96-tube (8 x 12) manifold
  • Dual-Action™
D Deep well microplates
  • Accomodates ANSI/SBS standard and deep well microplates <50 mm in height
CW Optimized cell washing for loosely adherent monolayers  

 

General
Microplate Types 96- and 384-well
Low profile and standard height
Deep well plates up to 50 mm
Onboard Software Create, edit or run multiple protocols
Software (pc control) LHC2 pc software
LHC2 Secure for 21 CFR Part 11 compliance (option)
SiLA Compliant driver (option)
Shaking Programmable up to 60 minutes
Slow, medium, fast or variable
Soaking Programmable up to 60 minutes
Automation BioStack and 3rd party automation compatible
Washing
Manifold Types 96-tube Dual-Action manifold for 96- & 384-well washing
Volume Range 50 - 3000 µL/well, in 1 µL increments
Wash Cycles 1 - 10
Buffer/reagent selection Auto switching module for up to 4 buffers (option)
Supply Bottle 4 L or 10 L (optional)
Dispense Precision ≤3% CV: 300 µL/well (96-well washing)
Residual Volume <2 µL/well (standard & deep well 96-well plates)
Wash Speed 96-wells, 300 µL/well, 3 cycles: <30 seconds
384-wells,100 µL/well, 3 cycles: <80 seconds
Flow Rates High flow to low flow
Optimized rates for cell assays
Sterilization Chemical
Vacuum Filtration Selectable: Approximate final at 30 seconds:
Lowest: -38 mmHg
Low: -113 mmHg
Medium: -200 mmHg
High: -390 mmHg
Highest: -506 mmHg

Vacuum filtration time range: 5 to 999 seconds
Physical Characteristics
Power 100 - 240 Volts AC. 50/60 Hz
Dimensions 14" W x 17" D x 11" H
(35.6 x 43.2 x 27.9 cm)
Weight 30 lbs (13.5 kg)
Regulatory
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Liquid Handling Control™ Software

Liquid Handling Control™ Software

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.


Standard (Included)

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Optional

Bottles & Tubing

7100531 Cap for Waste Bottle, 4L (Also fits 10L or 20L) accessory has picture
7100532 Spare Cap for 4L Dispense Bottle accessory has picture
7100533 Waste Tubing Set accessory has picture
7100534 Waste Bottle, 4L accessory has picture
7100535 Spare 4L Dispense Bottle Assembly accessory has picture
7100538 Dispense Tubing Set –1 Buffer accessory has picture
7100541S Dispense System Complete Assembly, 4L
7100542 Waste Bottle with Level Sensing, 4L accessory has picture
7100543S Waste Set with Level Sensing, 4L Bottles accessory has picture
7100544 Cap for Waste Bottle with Level Sensing, 4L accessory has picture
7100556 Waste Bottle and Tubing, 20L accessory has picture
7100557 Waste Bottle and Tubing, 10L accessory has picture
7100559 Dispense Bottle (10L) accessory has picture

Cables

75110 Serial cable, 9-pin male to 9-pin male

Dispense/Waste Systems

7100581S Dispense System Complete Assembly, 10L accessory has picture
7100582S Vacuum Set with Level Sense Assembly, 10L accessory has picture
7100745S Complete Dispense/Waste System 115/230V, 4L Bottles accessory has picture
7100746S Complete Waste System 115V/230V, 4L Bottles accessory has picture
7100751 Complete Dispense/High Flow Waste System 115V/230V, 4L Bottles accessory has picture
7100752 Complete Dispense/High Flow Waste System 115/230V, 10L Bottles accessory has picture
7100753S Complete High Flow Waste System 115V/230V, 4L Bottles
7100754 High Flow Vacuum Pump 115/230V
7100763 Complete Dispense/High Flow Waste System 115V/230V, 10L bottles
7103034 Standard Vacuum Pump 115V/230V accessory has picture

Filters

49943 Dispense Fluid Inlet Screen
48294 Filter for Waste Tubing accessory has picture

Miscellaneous

01113 Silencing Muffler for Standard Vacuum Pump
4030551 Vacuum Gauge/Regulator
7100025S Stylus Kit for any 405™/ELx405™ Select Deep Well manifold accessory has picture
7100096 15' extension kit for use with robotic system integrations of Elx405 Select Deep Well, EL406
7102215 384-well Ring Magnet accessory has picture
7102216 96-well Ring Magnet accessory has picture
7103016 96-well Flat Magnet accessory has picture
7103017 384-well Flat Magnet accessory has picture
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture

Product Qualification Packages

7100567 ELx405 Qualification Package - IQ/OQ/PQ

Test Plates & Reagents

7773001 BioTek Solution #2 10X Concentrate Blue Test Dye (125 mL)
7773002 BioTek Solution #1 100X Concentrate Wetting Agent (125 mL)

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