EL406 Microplate Washer Dispenser
The EL406 Combination Washer Dispenser offers fast, full plate washing along with three reagent dispensers in one, compact instrument… all from the recognized industry leader. The EL406 is the latest advancement in 1536-, 384- and 96-well microplate washing and dispensing incorporating BioTek’s patented Dual-Action™ manifold, optimized washing for loosely adherent cell monolayers, built-in patent-pending Ultrasonic Advantage™ for unattended wash manifold maintenance and up to four wash buffers for complex wash routines.
BioTek’s EL406 magnetic bead washing module offers high strength biomagnetic separation in both 384- and 96-well microplates. Based upon what is regarded as the industry standard in microplate washing, BioTek’s EL406™ Microplate Washer Dispenser offers full plate washing of magnetic microspheres used in a growing number of multiplex assays and bead-based ELISAs along with reagent dispensing. Developed in conjunction with leaders in genotyping, gene expression and protein assays built upon the Luminex® xMAP® platform, BioTek’s magnets incorporate high-energy neodymium iron boron magnets for rapid separation of micrometer and nanometer beads with superior retention. An accessory magnet adapter kit allows any EL406 configuration to accommodate magnetic bead assay washing and dispensing. An available vacuum filtration module makes the EL406 also well suited for polystyrene bead assays and filtration-to-waste processes. The EL406 Washer Dispenser is Luminex® xMAP® approved.
Up until now scientists had to choose their microplate dispenser technology – usually between either a peristaltic or syringe pump. Each of these technologies has its unique advantages. The EL406 eliminates the need to choose, offering both dispenser technologies on a single platform plus microplate washing. Now you can simply press a button and walk away. Automate an entire process by adding a BioStack™ Microplate Stacker.
The EL406 can be used for applications such as:
- ELISA Automation
- MSD Automation
- High Content Screening Immunocytochemistry
- Label-free Cell-based Assays – especially 1536-well density
- FLIPR® Ca2+ flux
- Magnetic and Polystyrene Bead Assay Automation for gene- and protein-expression
xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®
- 1536-, 384- and 96-well microplates
- Magnetic and polysyrene microsphere assays
- ELISAs and cell-based assays
- High-energy neodymium iron boron magnets
- Selection of optimized field gradients within wells
- Rapid separation of micrometer and nanometer beads with superior retention
- Fast and efficient vacuum filtration
- Fast microplate washing and dispensing
- Patented Dual-Action™ wash manifold
- Up to three reagent dispensers
- Unattended operation for long and tedious liquid handling processes
- Smaller benchtop or robotic system footprint compared to separate washer and three dispensers
- BioStack™ compatible for automated processing of up to 50 standard height microplates
- Choice of software control from instrument keypad or Liquid Handling Control™ (LHC) PC software
- Built-in ultrasonic cleaner
- Automatic switching of up to four wash buffers or reagents
| Model | EL406™ | ||||||||
| Part # | 1536-/ 384-/ 96-well | 1536-/ 384-well | 1536-well | 384-/ 96-well | 384-well | 96-well | 3 reagent disp. | 2 reagent disp. | 1 reagent disp. |
| 406PSUB3SB | • | • | |||||||
| 406PSUB3LB | • | • | |||||||
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| 406PSUB1LB | • | • | |||||||
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| 406PSU0LB | • | • | |||||||
| 406SNAOLB | • | • | |||||||
| 406PSUB3 | • | • | |||||||
| 406PSUB1 | • | • | |||||||
| 406PUB3 | • | • | |||||||
| 406PUB1 | • | • | |||||||
| 406PSUB2 | • | • | |||||||
| 406PUB2 | • | • | |||||||
| 406PSNA4 | • | • | |||||||
| 406SNA4 | • | • | |||||||
| 406P4 | • | • | |||||||
| EL406™ 1536-/384-/96-well Microplate Washer Dispenser Configurations |
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| Feature | Design | |
| P | 1-Reagent Dispense Module – 1 Peristaltic Pump |
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| S | 2-Reagent Dispense Module – 2 Syringe Pumps |
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| SNA | 2-Reagent Dispense Module – 2 Syringe Pumps |
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| U | Wash Module – Ultrasonic Advantage™ | |
| B | Wash Module – Automatic 4-Buffer Switching | |
| 1 | Wash Module – 384-/96-well microplate washing |
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| 2 | Wash Module – faster 384-well microplate washing |
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| 3 | Wash Module – 384-/96-well microplate washing |
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| 4 | Wash Module – 96-well microplate washing |
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| SB | Wash Module – 1536-well microplate washing and dispensing |
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| LB | Wash Module – 1536-well microplate washing and dispensing |
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| General | |
| Assays | Magnetic bead, polystyrene bead (optional)
Cell-based assays (model dependent) Filtration-to-waste processes (optional) |
| Separation | Biomagnetic separation, vacuum filtration (optional) |
| Microplate Types | 96-, 384- and 1536-well (model dependent) Low profile and standard height Solid and filter bottom (optional) |
| Shaking | Programmable in minutes and seconds, up to 60 minutes Intensities - slow, medium, fast or variable |
| Soak Time | Programmable in minutes and seconds, up to 60 minutes |
| Magnet | High strength 96- and 384-well designs Flat - Flat-bottom well - beads pulled to band across well bottom - Round-bottom well - beads pulled to button at well bottom Ring - beads pulled to 4-zone ring at well bottom |
| Safety/Convenience/Maintenance | Waste and vacuum sensing, fluid and flow detection Aerosol cover Adjustment utility for plate positioning Overflow protection Pre-programmed maintenance routines |
| Automation | BioStack™ Microplate Stacker - up to 50 SBS standard height plates (optional)
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| Onboard Software | 2 x 24 character LCD display 25 alphanumeric soft keys Create, edit or run multiple protocols |
| Software | Liquid Handling Control™, for PC protocol programming and execution (optional) |
| Operating Temperature | 15°C to 35°C (59°F to 95°F) |
| Humidity | Maximum 80% relative humidity at temperatures up to 31°C (87.8°F) decreasing linearly to 50% at 40°C (104°F) |
| Electrical | Compatible with 100 to 240 V~ ± 10% @ 50-60 Hz |
| Washing | |
| Manifold Types | 96-well washing: 96-tube (8x12) manifold - 316 stainless steel tubes 96-/384-well washing: Dual-Action 96-tube (8 x 12) manifold - 316 stainless steel tubes 384-well washing: Dual-Action 192-tube (16 x 12) manifold - 316 stainless steel tubes 1536-well washing: 2 32-tube (1 x 32) dispense manifolds - sapphire jeweled or 316 stainless steel tubes 128-tube (4 x 32) aspiration manifold |
| Washing Speed | Solid bottom plates 1 asp. / disp. cycle: 300 µL/well, 96 wells, 96-tube manifold: 13 seconds 100 µL /well, 384 wells, 192-tube manifold: 17 seconds 10 µL /well, 1536 wells, two 32-tube manifolds: 36 seconds Filter bottom plates: Variable, based on wash parameters |
| Fluid Delivery | 96- and 384-well washing: One positive displacement pump 1536-well washing: Two positive displacement syringe drives |
| Vacuum Filtration | Selectable vacuum levels: 0 to -380 mmHg (final at 30 seconds) Vacuum filtration time range: 5 - 999 seconds |
| Volume Range | 1 - 3,000 µL/well (model dependent) |
| Buffer Selection | Automatic switching for up to 4 wash buffers (model dependent) |
| Flow Rates | 1 - 11, including low flow cell wash rates (model dependent) |
| Wash Cycles | 1 - 250 |
| Dispense Accuracy | +3% typical |
| Dispense Precision | <3% CV (model dependent) |
| Residual Volume | <2 µL/well |
| Sterilization | Chemical |
| Ultrasonic Cleaning | Built-in ultrasonic bath for protein and salt crystal buildup Programmable in hours and minutes, up to 24 hours (model dependent) |
| Supply Bottle Volume | 4 L or 10 L (optional) |
| Dispensing - Peristaltic pump | |
| Manifold Types | 8-tip cassette (1 x 8) - sapphire jeweled 316 stainless steel, 316 stainless steel or polypropylene plastic tips |
| Dispensing Speed | 10 µL /well, 96 wells, 8-tip cassette: 3 seconds 5 µL /well, 384 wells, 8-tip cassette: 6 seconds 1 µL /well, 1536 wells, 8-tip cassette: 20 seconds 500 nL /well, 1536 wells, 8-tip cassette: 17 seconds 500 nL /well, 384 wells, 8-tip cassette: 6 seconds |
| Volume Range | 500 nL - 3,000 µL/well (model dependent) Selectable in 1 µL increments |
| Fluid Delivery | One positive displacement peristaltic pump |
| Flow Rates | Low, medium or high |
| Cassette Size | 1 µL: Recommended Volume Range: 500 nL - 50 µL Dispense Accuracy: +5% typical at 1 µL Dispense Precision: <5% CV typical at 1 µL <10% CV at 500 nL Minimum Prime Volume (30" tubing length): 1.20 mL 5 µL: Recommended Volume Range: 5 - 2,500 µL Dispense Accuracy: +2.5% typical at 5 µL Dispense Precision: <2.5% CV typical at 5 µL Minimum Prime Volume (30" tubing length): 4.23 mL 10 µL: Recommended Volume Range: 10 - 3,000 µL Dispense Accuracy: + 2.0% typical >-10 µL Dispense Precision: <-2.0% typical >-10 µL Minimum Prime Volume (30" tubing length): 7.36 mL |
| Recommended Replacement Interval | 1 µL Cassette: 1,000 384-well microplates at 5 µL / well 5 µL Cassette: 1,000 96-well microplates at 50 µL / well 10 µL Cassette: 1,000 96-well microplates at 100 µL / well |
| Sterilization | Autoclave, chemical |
| Dispensing - Syringe pump | |
| Manifold Types | 96-well dispensing: 1 16-tube (2 x 8) manifold - 316 stainless steel tubes 96-/384-well dispensing: 2 16-tube (1 x 16) manifolds - 316 stainless steel tubes 1536-well dispensing: 2 32-tube (1 x 32) manifolds - sapphire jeweled 316 stainless steel or 316 stainless steel tubes |
| Dispensing Speed | 10 µL /well, 96 wells, 1 x 16 or 1 x 8 tubes: 9 seconds 5 µL /well, 384 wells, 1 x 16 tubes: 11 seconds 3 µL /well 1536 wells, 2 x 32 tubes: 14 seconds |
| Volume Range | 3 - 3,000 µL/well (model dependent) Selectable in 1 µL increments Minimum Prime Volume: 12 mL |
| Fluid Delivery | Two positive displacement syringe drives |
| Flow Rates | 1 - 5 |
| Dispense Accuracy | +1 µL typical at 5 µL +1 µL typical at 20 µL +1% typical at 100 µL |
| Dispense Precision | <5% CV typical at 5 µL <2.5% CV typical at 20 µL <1% CV typical at 100 µL |
| Reagent Selection | Automatic switching for up to 4 reagents (optional) |
| Supply Bottle Volume | 1 L or 2 L (model dependent) |
| Sterilization | Autoclave (model dependent), chemical |
| Physical Characteristics | |
| Dimensions | Depth: 18" (46 cm) Width: 16.5" (42 cm) Height: 12.5" (32 cm) |
| Weight | 32 lbs (14.5 kg) |
| Regulatory | |
| Regulatory | CE and TUV marked. In Vitro Diagnostic use models are available. |
Specifications are subject to change.
Optional
BioStack3 Microplate Stacker
BioStack3 is the fastest stacker available to integrate with BioTek microplate instruments including washers, dispensers and readers. The new design allows compact, walk-away automation for benchtop or laboratory enclosure use.
Liquid Handling Control Software
Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.
Standard (Included) | ||
Operators Manual | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |
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