EL406 Combination Washer Dispenser
The EL406 Combination Washer Dispenser offers fast, full plate washing along with three reagent dispensers in one, compact instrument… all from the recognized industry leader. The EL406 is the latest advancement in microplate washing and dispensing incorporating BioTek’s patented Dual-Action™ manifold, optimized washing for loosely adherent cell monolayers, built-in patent-pending Ultrasonic Advantage™ for unattended wash manifold maintenance and up to four wash buffers for complex wash routines.
BioTek’s newly released EL406 magnetic bead washing module offers high strength biomagnetic separation in both 384- and 96-well microplates. Based upon what is regarded as the industry standard in microplate washing, BioTek’s EL406™ Microplate Washer Dispenser now offers full plate washing of magnetic microspheres used in a growing number of multiplex assays and bead-based ELISAs along with reagent dispensing. Developed in conjunction with leaders in genotyping, gene expression and protein assays built upon the Luminex xMAP® platform, BioTek’s magnets incorporate high-energy neodymium iron boron magnets for rapid separation of micrometer and nanometer beads with superior retention. An accessory magnet adapter kit allows any EL406 configuration to accommodate magnetic bead assay washing and dispensing.
Up until now scientists had to choose their microplate dispenser technology – usually between either a peristaltic or syringe pump. Each of these technologies has its unique advantages. The EL406 eliminates the need to choose, offering both dispenser technologies on a single platform plus microplate washing. Now you can simply press a button and walk away. Automate the entire process by adding a BioStack™ Microplate Stacker.
The EL406 can be used for applications such as:
- ELISA Automation
- MSD Automation
- High Content Screening Immunocytochemistry
- Label-free Cell-based Assays – especially 1536-well density
- FLIPR Ca2+ flux
- Magnetic Bead Assay Automation for gene- and protein-expression
- Accommodates 1536-, 384- and 96-well microplates
- Magnetic particle assays and standard ELISAs
- High-energy neodymium iron boron magnets
- Optimized field gradient within wells
- Rapid separation of micrometer and nanometer beads
- Superior microsphere retention
- Fast microplate washing and dispensing
- Patented Dual-Action™ wash manifold
- Up to three reagent dispensers
- Unattended operation for long and tedious liquid handling processes
- Smaller benchtop or robotic system footprint compared to separate washer and three dispensers
- BioStack™ compatible for automated processing of up to 50 standard height microplates
- Choice of software control from instrument keypad or Liquid Handling Control™ (LHC) PC software
- Built-in ultrasonic cleaner
- Automatic buffer switching of up to four wash buffers
| Model | EL406™ | ||||||
| Part # | 1536-/ 384-/ 96-well | 1536-/ 384-well | 1536-well | 384-/ 96-well | 384-well | 3 reagent disp. | 1 reagent disp. |
| 406PSUB3-SB | • | • | |||||
| 406PSUB3-LB | • | • | |||||
| 406PSUB1-SB | • | • | |||||
| 406PSUB1-LB | • | • | |||||
| 406PSUB2-SB | • | • | |||||
| 406PSUB2-LB | • | • | |||||
| 406PSU0-SB | • | • | |||||
| 406PSU0-LB | • | • | |||||
| 406PSUB3 | • | • | |||||
| 406PSUB1 | • | • | |||||
| 406PUB3 | • | • | |||||
| 406PUB1 | • | • | |||||
| 406PSUB2 | • | • | |||||
| 406PUB2 | • | • | |||||
All models include 1 peristaltic pump reagent dispenser module and Ultrasonic Advantage for unattended wash module manifold maintenance. All 384-/96-well and 384-well models include automatic wash module four-buffer switching.
| EL406™ 1536-/384-/96-well Microplate Washer Dispenser Configurations |
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| Feature | Design | |
| P | 1-Reagent Dispense Module – 1536-/384-/96-well microplate dispensing |
1 Peristaltic Pump
|
| S | 2-Reagent Dispense Module – 384-/96-well microplate dispensing |
2 Syringe Pumps
|
| U | Wash Module – Ultrasonic Advantage™ | |
| B | Wash Module – Automatic 4-Buffer Switching | |
| 1 | Wash Module – 384-/96-well microplate washing |
|
| 2 | Wash Module – faster 384-well microplate washing |
|
| 3 | Wash Module – 384-/96-well microplate washing |
|
| SB | Wash Module – 1536-well microplate washing and dispensing |
|
| LB | Wash Module – 1536-well microplate washing and dispensing |
|
| General | |
| Assays | Magnetic bead
|
| Microplate Types | Magnetic bead
|
| Shaking | Programmable in minutes and seconds, up to 60 minutes Intensities - slow, medium, fast or variable |
| Soak Time | Programmable in minutes and seconds, up to 60 minutes |
| Magnet | Custom LifeSep™design from Dexter Magnetic Technologies 384- and 96-well formats Optimized for flat-bottom microplates Removable for non-magnetic washing |
| Magnetic Field | 384-well separation 4,300 Gauss, on magnet surface 96-well separation 6,800 Gauss, on magnet surface |
| Safety/Convenience/Maintenace | Waste and vacuum sensing, fluid and flow detection Aerosol cover Adjustment utility for plate positioning Overflow protection Pre-programmed maintenance routines |
| Automation | BioStack™ Microplate Stacker compatible for unattended delivery of up to 50 plates |
| Onboard Software | 2 x 24 LCD character display 25 alphanumeric soft keys Create, edit or run multiple protocols |
| PC Software | Liquid Handling Control™ (optional), for PC protocol programming and execution EL406 Interface Software (optional), for robotic system integration EL406 Interface Software, for robotic system integration (optional) |
| Operating Temperature | 15°C to 35°C (59°F to 95°F) |
| Humidity | Maximum 80% relative humidity at temperatures up to 31°C (87.8°F) decreasing linearly to 50% at 40°C (104°F) |
| Electrical | Compatible with 100 to 240 V~ ± 10% @ 50-60 Hz |
| Washing | |
| Manifold Type | 1536-well washing: 2 32-tube (1 x 32) dispense manifolds - sapphire jeweled or steel tubes 128-tube (4 x 32) aspiration manifold 384-/96-well washing: Dual-Action™ 96-tube (8 x 12) manifold - steel tubes 384-well washing: Dual-Action™ 192-tube (16 x 12) manifold - steel tubes |
| Washing Speed | 1 asp. / disp. cycle: 300 µL/well, 96 wells, 96-tube manifold: 13 seconds 100 µL /well, 384 wells, 192-tube manifold: 17 seconds 10 µL /well, 1536 wells, 2 32-tube manifolds: 36 seconds |
| Fluid Delivery | 1536-well washing 2 microprocessor-controlled syringe drives 384-/96-well washing Internal positive displacement pump |
| Volume Range | 3 - 3,000 µL/well Selectable in 1 µL increments |
| Buffer Selection | Automatic switching for up to 4 wash buffers |
| Flow Rates | 1 - 11 Includes low flow, cell wash rates |
| Wash Cycles | 1 - 250 |
| Dispense Accuracy | +/-3% typical |
| Dispense Precision | <3% CV |
| Residual Volume | < 2 µL/well |
| Sterilization | Chemical |
| Ultrasonic Cleaning | Built-in ultrasonic bath for protein and salt crystal buildup Programmable in hours and minutes, up to 24 hours |
| Dispensing - Peristaltic pump | |
| Manifold Type | 8-tip cassette (1 x 8) - sapphire jeweled, steel or plastic tips |
| Dispensing Speed | 10 µL /well, 96 wells, 8-tip cassette: 8 seconds 5 µL /well, 384 wells, 8-tip cassette: 12 seconds 1 µL /well, 1536 wells, 8-tip cassette: 27 seconds |
| Volume Range | 1 - 3,000 µL/well Selectable in 1 µL increments |
| Fluid Delivery | Positive displacement peristaltic pump |
| Flow Rates | Low, medium or high |
| Cassette Size | 1 µL: Recommended Volume Range: 1 µL to 50 µL Dispense Accuracy: +/-5% typical at 1 µL Dispense Precision: <5% CV typical at 1 µL Dead Volume (30" tubing length): 1mL 5 µL: Recommended Volume Range: 5 µL to 2,500 µL Dispense Accuracy: +/-2.5% typical at 5 µL Dispense Precision: <2.5% CV typical at 5 µL Dead Volume (30" tubing length): 4 mL 10 µL: Recommended Volume Range: 10 µL to 3,000 µL Dispense Accuracy: +/-2.5% typical >10 µL Dispense Precision: <2.5% CV typical >10 µL Dead Volume (30" tubing length): 7 mL |
| Recommended Replacement Interval | 1 μL Cassette: 1,000 384-well microplates at 5 μL per well 5 μL Cassette: 1,000 96-well microplates at 50 μL per well 10 μL Cassette: 1,000 96-well microplates at 100 μL per well |
| Sterilization | Autoclave: Up to 50 cycles at temperatures and pressures of 121° C and 1 bar (750 mmHg) Chemical: 70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution |
| Dispensing - Syringe pump | |
| Manifold Type | 1536-well dispensing: 2 32-tube (1 x 32) manifolds - sapphire jeweled or steel tubes 384-/96-well dispensing: 2 16-tube (1 x 16) manifolds - steel tubes |
| Dispensing Speed | 10 µL /well, 96 wells, 16-tube manifold: 9 seconds 5 µL /well, 384 wells, 16-tube manifold: 11 seconds 3 µL /well 1536 wells, 2 32-tube manifolds: 14 seconds |
| Volume Range | 3 - 3,000 µL/well Selectable in 1 µL increments |
| Fluid Delivery | 2 microprocessor-controlled syringe drives |
| Flow Rates | 1 - 5 |
| Dispense Accuracy | +/-1 µL typical at 5 µL +/-1 µL typical at 20 µL +/-1% typical at 100 µL |
| Dispense Precision | <10% CV typical at 5 µL <5% CV typical at 20 µL <2% CV typical at 100 µL |
| Number of Reagents | 2 reagents |
| Dead Volume | 12 mL |
| Physical Characteristics | |
| Dimensions | 18"D x 16.5"W x 12.5"H (46 x 42 x 32 cm) |
| Weight | 32 lbs (14.5 kg) |
| Regulatory | |
| Regulatory | All BioTek microplate instrumentation is CE and ETL marked |
Specifications are subject to change.
Optional
BioStack Microplate Stacker
BioStack Twister II Microplate Handler
Liquid Handling Control Software
Standard (Included) | ||
Operators Manual | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |
