EL406 Washer Dispenser

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Combines fast microplate washing with 3 reagent dispensers in 1 compact instrument

96-, 384- and 1536-well plates

Full automation of intra-assay liquid handling steps

The EL406 Combination Washer Dispenser offers fast, full plate washing along with three reagent dispensers in one, compact instrument… all from the recognized industry leader. The EL406 is the latest advancement in 1536-, 384- and 96-well microplate washing and dispensing incorporating BioTek’s patented Dual-Action™ manifold, optimized washing for loosely adherent cell monolayers, built-in patented Ultrasonic Advantage™ for unattended wash manifold maintenance and up to four wash buffers for complex wash routines.

BioTek’s EL406 magnetic bead washing module offers high strength biomagnetic separation in both 384- and 96-well microplates. Based upon what is regarded as the industry standard in microplate washing, BioTek’s EL406™ Microplate Washer Dispenser offers full plate washing of magnetic microspheres used in a growing number of multiplex assays and bead-based ELISAs along with reagent dispensing. Developed in conjunction with leaders in genotyping, gene expression and protein assays built upon the Luminex® xMAP® platform, BioTek’s magnets incorporate high-energy neodymium iron boron magnets for rapid separation of micrometer and nanometer beads with superior retention. An accessory magnet adapter kit allows any EL406 configuration to accommodate magnetic bead assay washing and dispensing. An available vacuum filtration module makes the EL406 also well suited for polystyrene bead assays and filtration-to-waste processes. The EL406 Washer Dispenser is Luminex® xMAP® approved.

Up until now scientists had to choose their microplate dispenser technology - usually between either a peristaltic or syringe pump. Each of these technologies has its unique advantages. The EL406 eliminates the need to choose, offering both dispenser technologies on a single platform plus microplate washing. Now you can simply press a button and walk away. Automate an entire process by adding a BioStack™ Microplate Stacker.

The EL406 can be used for applications such as:

  • ELISA Automation
  • MSD Automation
  • High Content Screening Immunocytochemistry
  • Label-free Cell-based Assays – especially 1536-well density
  • FLIPR® Ca2+ flux
  • Magnetic and Polystyrene Bead Assay Automation for gene- and protein-expression

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®

 

  • 1536-, 384- and 96-well microplates
  • Magnetic and polysyrene microsphere assays
  • ELISAs and cell-based assays
  • High-energy neodymium iron boron magnets
  • Selection of optimized field gradients within wells
  • Rapid separation of micrometer and nanometer beads with superior retention
  • Fast and efficient vacuum filtration
  • Fast microplate washing and dispensing
  • Patented Dual-Action™ wash manifold
  • Up to three reagent dispensers
  • Unattended operation for long and tedious liquid handling processes
  • Smaller benchtop or robotic system footprint compared to separate washer and three
  • dispensers
  • BioStack™ compatible for automated processing of up to 50 standard height microplates
  • Choice of software control from instrument keypad or Liquid Handling Control™ (LHC) PC
  • software
  • Built-in ultrasonic cleaner
  • Automatic switching of up to four wash buffers or reagents

 

Part # 1536-/ 384-/ 96-well 1536-/ 384-well 1536-well 384-/ 96-well 384-well 96-well 3 reagent disp. 2 reagent disp. 1 reagent disp.
406PSUB3SB              
406PSUB3LB              
406PSUB1SB              
406PSUB1LB              
406PSUB2SB              
406PSUB2LB              
406PSU0SB              
406PSU0LB              
406SNA0LB              
406PSUB3              
406PSUB1              
406PUB3              
406PUB1              
406PSUB2              
406PUB2              
406PSNA4              
406SNA4              
406P4              

 

EL406™ 1536-/384-/96-well Microplate Washer Dispenser Configurations
  Feature Design
P 1-Reagent Dispense Module –
1 Peristaltic Pump
  • Autoclavable
  • 8-tip (1 x 8) cassette
  • Sapphire jeweled, steel or plastic tips
S 2-Reagent Dispense Module –
2 Syringe Pumps
  • Autoclavable
  • 2 16-tube (1 x 16) manifolds
  • Steel tubes
SNA 2-Reagent Dispense Module –
2 Syringe Pumps
  • Non-autoclavable
  • 1 16-tube (2 x 8) manifolds - except with P/N 406SNA0-LB
  • Steel tubes
U Wash Module – Ultrasonic Advantage™  
B Wash Module – Automatic 4-Buffer Switching  
1 Wash Module – 384-/96-well microplate washing
2 Wash Module – faster 384-well microplate washing
  • Dual-Action™
  • 192-tube (16 x 12) manifold
  • Steel tubes
3 Wash Module – 384-/96-well microplate washing
  • Dual-Action™
  • Interchangeable 96-tube (384-/96- well washing) and 192-tube (faster 384-well washing) manifolds
  • Steel tubes
4 Wash Module – 96-well microplate washing
  • 96-tube (8 x 12) manifold
  • Steel tubes
SB Wash Module – 1536-well microplate washing and dispensing
  • 2 32-tube (1 x 32) dispense manifolds
  • Small bore, sapphire jeweled tubes
  • 128-tube (4 x 32) aspiration manifold
LB Wash Module – 1536-well microplate washing and dispensing
  • 2 32-tube (1 x 32) dispense manifolds
  • Large bore, steel tubes
  • 128-tube (4 x 32) aspiration manifold

 

General
Assays ELISA
Cell-based assays (model dependent)
Magnetic bead, polystyrene bead (optional)
  • Multiplex assays
  • Bead-based ELISA
Filtration-to-waste processes (optional)
Shaking Programmable in minutes and seconds, up to 60 minutes
Intensities - slow, medium, fast or variable
Soak Time Programmable in minutes and seconds, up to 60 minutes
Separation Biomagnetic separation, vacuum filtration (optional)
Microplate Types 96-, 384- and 1536-well (model dependent)
Low profile and standard height
Solid and filter bottom (optional)
Safety/Convenience/Maintenance Waste and vacuum sensing, fluid and flow detection
Aerosol cover
Adjustment utility for plate positioning
Overflow protection
Pre-programmed maintenance routines
Magnet High strength 96- and 384-well designs
Flat
- Flat-bottom well - beads pulled to band across well bottom
- Round-bottom well - beads pulled to button at well bottom
Ring - beads pulled to 4-zone ring at well bottom
Automation Compatible with BioStack (excluding filter bottom plates) and 3rd party automation
Onboard Software Create, edit or run multiple protocols
Software Liquid Handling Control™, for PC protocol programming and execution (optional). SiLA compliant driver available.
Washing
Manifold Types 96-well washing:
96-tube (8x12) manifold - 316 stainless steel tubes
96-/384-well washing:
Dual-Action 96-tube (8 x 12) manifold - 316 stainless steel tubes
384-well washing:
Dual-Action 192-tube (16 x 12) manifold - 316 stainless steel tubes
1536-well washing:
Two 32-tube (1 x 32) dispense manifolds -sapphire jeweled
316 stainless steel or 316 stainless steel tubes
128-tube (4 x 32) aspiration manifold
Washing Speed Solid bottom plates, 1 asp. / disp. cycle:
300 µL/well, 96 wells, 96-tube manifold: 13 seconds
100 µL /well, 384 wells, 192-tube manifold: 17 seconds
10 µL /well, 1536 wells, two 32-tube manifolds: 36 seconds
Fluid Delivery 96- and 384-well washing:
One positive displacement pump
1536-well washing:
Two positive displacement syringe drives
Vacuum Filtration Selectable vacuum levels: 0 to -380 mmHg
(final at 30 seconds)
Vacuum filtration time range: 5 - 999 seconds
Volume Range 3 - 3,000 µL/well (model dependent)
Selectable in 1 µL increments
Buffer Selection Automatic switching for up to 4 wash buffers (model dependent)
Flow Rates 1 - 11, including low flow cell wash rates (model dependent)
Wash Cycles 1 - 250
Dispense Precision <3% CV (model dependent)
Dispense Accuracy +3%
Residual Volume <2 µL/well
Sterilization Chemical
Ultrasonic Cleaning Built-in ultrasonic bath to remove protein and salt crystal buildup
Supply Bottle 4 L or 10 L (optional)
Dispensing - Peristaltic pump (Multi-Channel)
Manifold Types 8-tip cassette (1 x 8) - sapphire jeweled 316 stainless steel, 316 stainless steel or polypropylene plastic tips
Dispense Speed 10 µL /well, 96 wells, 8-tip cassette: 8 seconds
5 µL /well, 384 wells, 8-tip cassette: 12 seconds
1 µL /well, 1536 wells, 8-tip cassette: 27 seconds
Volume Range 500 mL - 3,000 µL/well (model dependent)
Selectable in 1 µL increments
Fluid Delivery One positive displacement peristaltic pump
Flow Rates Low, medium or high
Cassette Size 1 µL:
Recommended Volume Range: 1 - 50 µL
Dispense Accuracy: +5% at 1 µL
Dispense Precision: <5% CV at 1 µL
<10% CV at 500 nL
Minimum Prime Volume (30" tubing length): 1.20 mL

5 µL:
Recommended Volume Range: 5 - 2,500 µL
Dispense Accuracy: +2.0% at 5 µL
Dispense Precision: <2.5% CV at 5 µL
Minimum Prime Volume (30" tubing length): 4.23 mL

10 µL:
Recommended Volume Range: 10 - 3,000 µL
Dispense Accuracy: + 2.0% at 10 µL
Dispense Precision: <-2.0% CV at 10 µL
Minimum Prime Volume (30" tubing length): 7.36 mL
Sterilization Autoclave, chemical
Dispensing - Syringe pump
Manifold Types 96-well dispensing:
One 16-tube (2 x 8) manifold - 316 stainless steel tubes
96-/384-well dispensing:
Two 16-tube (1 x 16) manifolds - 316 stainless steel tubes
1536-well dispensing:
Two 32-tube (1 x 32) manifolds - sapphire jeweled 316 stainless steel or 316 stainless steel tubes
Dispense Speed 20 µL /well, 96 wells, 1 x 16 tubes: 5.25 seconds
20 µL /well, 384 wells, 1 x 16 tubes: 14 seconds
3 µL /well 1536 wells, 2 x 32 tubes: 7 seconds
Volume Range 3 - 3,000 µL/well
Selectable in 1 µL increments
Minimum Prime Volume: 12 mL
Fluid Delivery Two positive displacement syringe drives
Flow Rates 1 - 5
Dispense Accuracy +1 µL at 5 µL
+1 µL at 20 µL
+1% at 100 µL
Dispense Precision <5% CV at 5 µL
<2.5% CV at 20 µL
<1% CV at 100 µL
Reagent Selection Automatic switching for up to 4 reagents (optional)
Supply Bottle 1 L or 2 L (model dependent)
Sterilization Autoclave (model dependent), Chemical
Physical Characteristics
Power 100 - 240 Volts AC. 50/60 Hz.
Dimensions 16.5" W x 18" D x 12.5" H (42 x 46 x 32 cm)
Weight 32 lbs (14.5 kg)
Regulatory
Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Liquid Handling Control™ Software

Liquid Handling Control™ Software

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.


Standard (Included)

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Optional

BioStack Integration Kits

7310042 BioStack Integration Kit for EL406 accessory has picture

Bottles & Tubing

7100531 Cap for Waste Bottle, 4L (Also fits 10L or 20L) accessory has picture
7100532 Spare Cap for 4L Dispense Bottle accessory has picture
7100543S Waste Set with Level Sensing, 4L Bottles accessory has picture
7100544 Cap for Waste Bottle with Level Sensing, 4L accessory has picture
7100556 Waste Bottle and Tubing, 20L accessory has picture
7100557 Waste Bottle and Tubing, 10L accessory has picture
7100559 Dispense Bottle (10L) accessory has picture
7180541 Dispense Tubing Set - Teflon Lined
7183002 Supply Tubing Set for DMSO & Acetonitrile for 2 Syringe Pump Dispensers
7183006 Spare Tubing Set for 2 Syringe Pump Dispensers

Cassettes

7170007 Large Volume Replacement Tubing Set (10 µL) - molded plastic tips accessory has picture
7170008 Medium Volume Replacement Tubing Set (5 µL) - molded plastic tips accessory has picture
7170009 Low Volume Replacement Tubing Set (1 µL) - molded plastic tips accessory has picture
7170010 Large Volume (10 µL) Cassette - molded plastic tips accessory has picture
7170011 Medium Volume (5 µL) Cassette - molded plastic tips accessory has picture
7170012 Low Volume (1 µL) Cassette - molded plastic tips accessory has picture
7170013 Large Volume (10 µL) Cassette - stainless steel tips accessory has picture
7170014 Medium Volume (5 µL) Cassette - stainless steel tips accessory has picture
7170015 Low Volume (1 µL) Cassette - sapphire jeweled, stainless steel tips accessory has picture
7170016 Low Volume (1 µL) 1536-well Cassette accessory has picture
7170018 Low Volume (1 µL) 1536-well Cassette - molded plastic tips accessory has picture
7170020 Large volume (10 µL) Cassette Tubing Extension Kit accessory has picture
7170021 Medium volume (5 µL) Cassette Tubing Extension Kit accessory has picture
7170022 Low volume (1 µL) Cassette Tubing Extension Kit accessory has picture
7170023 Large Volume (10 µL) Cassette - large bore, molded plastic tips
7170024 Medium Volume (5 µL) Cassette - large bore, molded plastic tips

Dispense/Waste Systems

7100581S Dispense System Complete Assembly, 10L accessory has picture
7100582S Vacuum Set with Level Sense Assembly, 10L accessory has picture
7100745S Complete Dispense/Waste System 115/230V, 4L Bottles accessory has picture
7100746S Complete Waste System 115V/230V, 4L Bottles accessory has picture
7100751 Complete Dispense/High Flow Waste System 115V/230V, 4L Bottles accessory has picture
7100752 Complete Dispense/High Flow Waste System 115/230V, 10L Bottles accessory has picture
7100754 High Flow Vacuum Pump 115/230V
7103024 Standard Vacuum Pump 115V/230V accessory has picture

Filters

49943 Dispense Fluid Inlet Screen
48294 Filter for Waste Tubing accessory has picture

Manifolds

7180549S 8-tube (1x8) Large bore manifold for 96-well Microplates autoclavable

Miscellaneous

4030551 Vacuum Gauge/Regulator
7100025S Stylus Kit for any 405™/ELx405™ Select Deep Well manifold accessory has picture
7102215 384-well Ring Magnet accessory has picture
7102216 96-well Ring Magnet accessory has picture
7103016 96-well Flat Magnet accessory has picture
7103017 384-well Flat Magnet accessory has picture
7180006S 2 Syringe Pump Dispenser Module - Autoclavable Upgrade Kit
7180010 Peristaltic Pump Angled Dispense Kit accessory has picture
7180011 Magnet Adapter Kit
7180028 EL406 Dual Syringe Dispenser Automatic Reagent Switching Module accessory has picture
7180029 EL406 Vacuum Filtration Module Accessory accessory has picture
7182109 40 mL Capacity Dispenser Priming Trough accessory has picture
7210509 Spare Reservoir Holder and Strap
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture

Product Qualification Packages

7180527 EL406 Qualification Package

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