BioTek: Microplate Instrumentation & Software Solutions
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405 Touch Microplate Washer

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High resolution, advanced touch screen User Interface



USB flash drive ports for convenient file transfer and storage


Optimized for loosely adherent monolayers: low flow rates, angled dispensing



Patented Verify™ clog detection technology and Ultrasonic Advantage™

The 405 Touch Microplate Washer features the new patented* Verify™ technology, which runs an automated QC check for manifold tube blockage, and visually reports any failed wells.  The patent pending Ultrasonic Advantage™ can then be used to automatically and thoroughly clean the aspirate and dispense manifolds. The 405 Touch, with the unique Verify and Ultrasonic Advantage features, overcomes the common problem of undetected washer manifold clogging which can lead to assay failure. The 405 Touch is the first self-checking, self-maintaining microplate washer available!

The 405™ Touch Microplate Washer incorporates a high resolution touch screen user interface for intuitive and flexible programming of 96- and 384-well microplate wash protocols. The extensive onboard software seamlessly guides users through protocol development, instrument maintenance and operation. For those who prefer computer control or require 21 CFR Part 11 compliance, the 405 Touch can be controlled with optional LHC™ Software.

Cell-based assays and microsphere-based assays are easily run with the 405 Touch’s unique set of available features and modules for gentle washing vacuum filtration and biomagnetic separation. The 405 Touch is Luminex® xMAP® approved; a range of configurations is available for this and a broad variety of other microplate washing applications. Review the models and specifications sections for details on all the possible configurations, there’s certain to be one that fits your laboratory’s requirements.

With its color graphical user interface, fail-safe QC and maintenance tools, and compact, robot-compatible design, the 405 Touch is an easy to use microplate washing solution for a wide range of applications.

* EP 1 637 887


US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®

 

Hardware features and options:

  • Patented built-in Verify sensor automatically checks dispense / aspirate performance and reports clogged locations in the color graphical display.

Washer-Verifier

  • Accommodates 96- and 384-well microplates
  • High resolution touch screen user interface
  • Onboard computer running Microsoft® Windows® CE
  • Patent pending built-in Ultrasonic Advantage automatically cleans clogged tubes
  • Two USB flash drive ports enable convenient file transfer, storage and operation
  • Automated internal 4-buffer switching
  • Internal positive displacement pump eliminates use of troublesome pressure delivery systems and allows use of any size dispense reservoir
  • Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates need for blank plates when used in automated robotic systems
  • All washers vacuum filtration ready (accessory filtration modules sold separately)
  • All washers biomagnetic separation ready (accessory magnets sold separately)

405 Select TS

  • 96-/384-well washing with patented 96-tube Dual-Action™ manifold
  • Cell washing is optimized with two low-flow rates and 20° angled dispense tubes ideal for washing loosely adherent cell monolayers

dual-action-manifold

405 HT TS

  • Fast 384-well washing with patented 192-tube Dual-Action™ manifold for independent dispense and aspiration control for precise overfill washing and overflow protection:
  • Superior tube-in-tube aspiration manifold design for 384-well washing with buffers not containing surfactant
  • 96-well washing ready with optional 96-tube Dual-Action manifold

405 TS

  • 405 TS models automate basic 96-well dispensing and aspiration

Software Features:

  • Context sensitive help system complete with recommendations, diagrams and images is only a key press away when questions arise
  • Pre-defined protocols and instructional videos walk users through instrument setup and basic use
  • Fully customizable protocol parameters for individualized wash requirements - gentle cell-washing to vigorous wash for high-binding assays
  • AutoPrime
  • Additional software flexibility and 21 CFR Part 11 compliance with Liquid Handling Control™ (LHC) Software for method development, downloading and washing plates.
  • Run protocols created onboard, downloaded from LHC™ Software or transferred on USB drive
Part #
96-/384-well
384-well
96-well
Optimized Cell Washing
4-Buffer Switching
Ultrasonic Advantage
Verify™ technology
Biomagnetic Separation*
Vacuum Filtration*
405TSHT
      
 
405TSHTV
   
 
405TSU
    
     
405TSUV
 
405TSUS
   
 
 
405TSUSQ
405TSUVS
   
 
405TSUVSQ
405TSR
 
   
405TSRV
    
 
   
405TSRS
   
 
 
405TSRSQ
   
   
405TSRVS
   
 
 
405TSRVSQ
   
 

* With available accessory module sold separately

 

405™ Touch Microplate Washer Configurations
Feature Design
HT Faster 384-well microplate washing
  • 192-tube (16 x 12) manifold
  • Dual-Action™
V Automated Internal 4-Buffer Switching  
S Ultrasonic Advantage™
  • Built-in ultrasonic cleaner for manifold self-maintenance
U 96-/384-well microplate washing
  • 96-tube (8 x 12) manifold
  • Dual-Action™
R 96-well microplate washing
  • 96-tube (8 x 12) manifold
Q Verify™ technology
  • Built-in clog detection for dispense and aspirate tubes

 

General
Microplate Types 96- and 384-well (model dependent)
Low profile and standard height
Solid and filter bottom
  • Filter pore sizes 0.45 µm to 1.2 µm
Assays ELISA
Cell-based assays (model dependent)
Magnetic bead, polystyrene bead
  • Multiplex assays
  • Bead-based ELISA

Filtration-to-waste processes
Separation Biomagnetic separation, vacuum filtration
Shaking Programmable in minutes and seconds, up to 60 minutes
Intensities - slow, medium, fast or variable
Soak Time Programmable in minutes and seconds, up to 60 minutes
Magnet High strength 96- and 384-well designs
Choice of two designs, custom for BioTek microplate washers:
Flat:
- Flat-bottom well - beads pulled to band across well bottom
- Round-bottom well - beads pulled to button at well bottom
Ring: - beads pulled to 4-zone ring at well bottom
Safety/Convenience/Maintenance Waste and vacuum sensing, fluid and flow detection
Aerosol cover
Adjustment utility for plate positioning
Overflow protection
Preprogrammed maintenance routines
Automation BioStack™ Microplate Stacker - up to 50 ANSI/SBS standard height plates (optional)
  • Except filter bottom plates
Onboard Software High resolution backlit LED touch screen UI
5.7 inch graphical color display
Intel Atom processor running Windows CE
Predefined sample wash protocols - ELISA, cell wash, biomagnetic separation and vacuum filtration
Quick Menu
Create or edit multiple custom protocols
Run protocols created onboard, downloaded from LHC™ Software or transferred on USB flash drive
Software Liquid Handling Control™ (LHC™), for PC wash protocol programming and execution (optional)
Operating Temperature 15° C to 30° C (59° F to 86° F)
Interface For PC control and BioStack™ integration – 1 USB port, 1 9-pin RS232 port
For file transfer – 2 USB Flash Memory ports
Humidity 10% to 85%, non-condensing
Electrical 100 – 240 Volts AC 50/60 Hz, < 8.0 A
Washing
Manifold Types 96-well washing:
96-tube (8 X 12) manifold
96-/384-well washing:
Dual-Action™ 96-tube (8 X 12) manifold
384-well washing:
Dual-Action™ 192-tube (16 x 12) manifold
Washing Speed Solid bottom plates:

96-Well:
3 asp. / disp. cycles, 300 µL/well, 96-tube manifold, final aspirate: <30 seconds
384-Well:
3 asp. / disp. cycles, 100 µL/well, 96-tube manifold, final aspirate: <80 seconds
1 asp. / disp. cycle, 400 µL/well, 384 wells, 192-tube manifold, final aspirate: <20 seconds


Filter bottom plates:
Variable, based on wash parameters
Fluid Delivery One internal positive displacement pump
Vacuum Filtration Selectable vacuum levels (approximate finals at 30 seconds):

Lowest: -38 mmHg
Low: -113 mmHg
Medium: -200 mmHg
High: -390 mmHg
Highest: -506 mmHg

Vacuum filtration time range:
5 to 999 seconds
Volume Range 25 to 3,000 µL/well
Selectable in 1 µL increments
Buffer Selection Internal automatic switching for up to 4 wash buffers (optional)
Flow Rates 1 to 11
Includes low flow, cell wash rates (model dependent)
Wash Cycles 1 to 250
Dispense Precision
  • 96-well washing - 96-tube (8 x 12) manifold:
    3% CV: 300 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate 6, absorbance read at 630 nm and 450 nm reference
  • 384-well washing - 192-tube (16 x 12) manifold:
    < 4% CV: 80 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate 7, absorbance read at 630 nm and 450 nm reference
Residual Volume Solid bottom plates:
  • 96-well washing - 96-tube (8 x 12) manifold:
2 µL/well: 3-cycle wash, 300 µL/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height
optimized prior to testing
  • 384-well washing - 192-tube (16 x 12) manifold:
< 2 µL/well: 3-cycle wash, 100 µL/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height optimized prior to testing

Filter bottom plates:
  • 96-well:
Average increase weight of plate < 1.2 g after dispensing 300 µL to
0.45 µm plate, filtration 30 seconds, low vacuum, blotted
  • 384-well:
Average increase weight of plate < 4.0 g after dispensing 80 µL to 1.2
µm plate, filtration 10 seconds, low vacuum, blotted
Sterilization Chemical
0.5% sodium hypochlorite, 70% isopropyl alcohol or 70% ethanol solution
Ultrasonic Cleaning Built-in ultrasonic bath for protein and salt crystal buildup
Programmable in hours and minutes, up to 60 minutes
(model dependent)
Clog Detection Built-in Verify™ technology detects dispense and aspirate tubes for blockage (model dependent)
Supply Bottle Volume 4 L or 10 L (optional)
Physical Characteristics
Dimensions Depth: 17" (43.2 cm)
Width: 14" (35.6 cm)
Height: 10" (25.4 cm)
Weight With internal buffer switching - 36 lbs (16.5 kg)
Without - 30 lbs (13.5 kg)
Regulatory
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.

Specifications are subject to change.

Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Liquid Handling Control™ Software

Liquid Handling Control™ Software

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.


Standard (Included)

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center

Optional

BioStack Integration Kits

7310040 BioStack Integration Kit for 405™ TS and 405™ LS

Bottles & Tubing

1173000 Dispense Bottle - with Filter, 10L accessory has picture
1173002 Cap for Dispense Bottle - with Filter, 10L
1173003 Cap for Dispense Bottle - with Filter, 4L accessory has picture
1173031 Dispense Bottle – with Filter, 4L accessory has picture
7100531 Cap for Waste Bottle, 4L (Also fits 10L or 20L) accessory has picture
7100533 Waste Tubing Set accessory has picture
7100534 Waste Bottle, 4L accessory has picture
7100537 Dispense Tubing Set – 4 Buffers
7100538 Dispense Tubing Set –1 Buffer accessory has picture
7100542 Waste Bottle with Level Sensing, 4L accessory has picture
7100543S Waste Set with Level Sensing, 4L Bottles accessory has picture
7100544 Cap for Waste Bottle with Level Sensing, 4L accessory has picture
7100556 Waste Bottle and Tubing, 20L accessory has picture
7100557 Waste Bottle and Tubing, 10L accessory has picture

Dispense/Waste Systems

1170528S Complete Dispense System, 10L Bottles accessory has picture
1170529S Complete Dispense System, 4L Bottles accessory has picture
1170530 Complete Dispense/Waste System 115V/230V, 4L Bottles accessory has picture
1170531S Complete High Flow Waste System 115V/230V, 10L Bottles
1170532 Complete Dispense/High Flow Waste System 115V/230V, 4L Bottles accessory has picture
1170533 Complete Dispense/High Flow Waste System 115V/230V, 10L Bottles
1170534S Complete Waste System 115V/230V, 10L Bottles
1170535 Complete Dispense/Waste System 115V/230V, 10L Bottles
1170536 Complete Dispense/Direct Drain Waste System 115V/230V, 4L Bottles accessory has picture
1170537 Complete Dispense/Direct Drain Waste System 115V/230V, 10L Bottles accessory has picture
7100582S Vacuum Set with Level Sense Assembly, 10L accessory has picture
7100746S Complete Waste System 115V/230V, 4L Bottles accessory has picture
7100753S Complete High Flow Waste System 115V/230V, 4L Bottles
7103024 Standard Vacuum Pump 115V/230V accessory has picture

Filters

01310 Filter for Dispense Bottle
48294 Filter for Waste Tubing accessory has picture

Manifolds

1170010 96-Tube Dual-Action Manifold Kit, with 70 dispense tubes and tube-in-tube aspiration manifold kit for 405 HT TS and 405 HT LS models only
1172046S 96-tube Dual-Action™ Tube-in-Tube Aspiration Manifold accessory has picture
7102068S 96-tube Dual-Action™ 7° Dispense Manifold

Miscellaneous

01113 Silencing Muffler for Standard Vacuum Pump
1170008 96-well Vacuum Filtration Kit accessory has picture
1170009 96-/384-well Vacuum Filtration Kit accessory has picture
4030551 Vacuum Gauge/Regulator
7100025S Stylus Kit for any 405™/ELx405™ Select Deep Well manifold accessory has picture
7102215 384-well Ring Magnet accessory has picture
7102216 96-well Ring Magnet accessory has picture
7103016 96-well Flat Magnet accessory has picture
7103017 384-well Flat Magnet accessory has picture
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture

Product Qualification Packages

1170522 405™ TS Qualification Package - IQ/OQ/PQ package on CD

Test Plates & Reagents

1240001 Replacement 405 Verify test plate kit accessory has picture

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