405 LS Washer

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Intuitive onboard User Interface or LHC™ pc software

Magnetic and polystyrene bead assays, conventional ELISAs

Patented Dual-Action™ manifold

The 405™ LS Microplate Washer is the fifth generation BioTek washer, known for superior performance and reliability. Built-in automated buffer switching and "quick-change" manifold designs provide improved ease of use and efficiency when washing 96 and 384 well microplates.

Washer maintenance is critical for excellent assay performance, and the 405 LS now offers the new patented* Verify™ Technology for clog detection and patented Ultrasonic Advantage™ for automated manifold cleaning, making the 405 LS a truly 'self-maintaining' system.

The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions. The optional BioStack™ is available for walk-away automation of up to 50 microplates.

Cell-based assays are easily processed with the 405 Select LS, with its angled dispense tubes, extensive aspirate and dispense rates and plate position controls providing optimal conditions for washing even fragile cell layers.  Magnetic separation and vacuum filtration modules are available for washing magnetic and polystyrene beads used in a growing number of multiplex assays and bead-based ELISAs, such as those developed on the Luminex® xMAP® technology platform. The vacuum filtration feature automates the wash steps of filter bottom plates with five different vacuum levels for optimal performance of filter pore sizes ranging from 0.45 µm to 1.2 µm. The 405 LS is Luminex® xMAP® approved.

A range of configurations for different microplate formats and assay requirements is available for microplate washing, each designed for optimal performance for your plate type and application.

* EP 1 637 887


US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®


  • Patented built-in Verify™ sensor controlled through Liquid Handling Control™ (LHC) pc software, automatically checks dispense/aspirate performance and reports clogged tubes:

  • Accommodates 96- and 384-well microplates
  • 2x24 character backlit LCD display and membrane keypad user interface
  • Patented built-in Ultrasonic Advantage™ automatically cleans clogged tubes
  • Automated internal 4-buffer switching
  • Internal positive displacement pump eliminates use of troublesome pressure delivery systems and allows use of any size dispense reservoir
  • Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates need for blank plates when used in automated robotic systems
  • Quick change manifold design
  • BioStack and 3rd party automation compatible
  • All washers biomagnetic separation ready (accessory magnets sold separately)
  • All washers vacuum filtration ready (accessory filtration modules sold separately)

405 Select LS

  • 96-/384-well washing with patented 96-tube Dual-Action™ manifold
  • Cell washing is optimized with two low-flow rates and 20° angled dispense tubes ideal for washing loosely adherent cell monolayers

405 HT LS

  • Fast 384-well washing with patented 192-tube Dual-Action™ manifold for independent dispense and aspiration control for precise overfill washing and overflow protection:
  • Superior tube-in-tube aspiration manifold design for 384-well washing with buffers not containing surfactant
  • 96-well washing ready with optional 96-tube Dual-Action manifold

405 LS

  • 405 LS models automate basic 96-well dispensing and aspiration


  • Quick menu allows fluid paths to be easily primed, wash process started and manifold cleaned
  • Predefined protocols included for out-of-box washing
  • Fully customizable protocol parameters for individualized wash requirements - gentle cell-washing to vigorous wash for high-binding assays
  • AutoPrime and auto maintenance routines
  • Additional software flexibility and 21CFR Part 11 compliance with LHC™ Software for method development, downloading and washing plates
  • Run protocols created onboard or downloaded from LHC Software
Part #
Optimized Cell Washing
4-Buffer Switching
Biomagnetic Separation*
Vacuum Filtration*


* With available accessory module sold separately


405™ LS Microplate Washer Configurations
  Feature Design
HT Faster 384-well microplate washing
V Automated Internal 4-Buffer Switching  
S Ultrasonic Advantage™
  • Built-in ultrasonic cleaner for manifold self-maintenace
U 96-/384-well microplate washing
  • 96-tube (8 x 12) manifold
  • Dual-Action™
R 96-well microplate washing
  • 96-tube (8 x 12) manifold
Q Verify™ Technology
  • Built-in clog detection for dispense and aspirate tubes



Assays ELISA
Cell-based assays (model dependent)
Magnetic bead, polystyrene bead
  • Multiplex assays
  • Bead-based ELISA

Filtration-to-waste processes
Shaking Programmable in minutes and seconds, up to 60 minutes
Intensities - slow, medium, fast or variable
Soak Time Programmable in minutes and seconds, up to 60 minutes
Separation Biomagnetic separation, vacuum filtration
Microplate Types 96- and 384-well (model dependent)
Low profile and standard height
Solid and filter bottom
  • Filter pore sizes 0.45 µm to 1.2 µm
Safety/Convenience/Maintenance Waste and vacuum sensing, fluid and flow detection
Aerosol cover
Adjustment utility for plate positioning
Overflow protection
Preprogrammed maintenance routines
Magnet High strength 96- and 384-well designs
Choice of two designs, custom for BioTek microplate washers:
- Flat-bottom well - beads pulled to band across well bottom
- Round-bottom well - beads pulled to button at well bottom
Ring: - beads pulled to 4-zone ring at well bottom
Automation BioStack™ Microplate Stacker - up to 50 ANSI/SBS standard height plates (optional)
  • Except filter bottom plates
Onboard Software 2 x 24 character backlit LCD display with membrane keypad UI
Predefined sample wash protocols - ELISA, cell wash, biomagnetic separation and vacuum filtration
Quick Menu
Create or edit multiple custom protocols
Run protocols created onboard, downloaded from LHC™ Software
Software Liquid Handling Control™ (LHC™), for PC wash protocol programming and execution (optional)
Operating Temperature 15° C to 30° C (59° F to 86° F)
Interface For PC control and BioStack™ integration - 1 USB port, USB and 9-pin RS232
Humidity 10% to 85%, non-condensing
Electrical 100 - 240 Volts AC 50/60 Hz, < 8.0 A
Manifold Types 96-well washing:
96-tube (8 X 12) manifold
96-/384-well washing:
Dual-Action™ 96-tube (8 X 12) manifold
384-well washing:
Dual-Action™ 192-tube (16 x 12) manifold
Washing Speed Solid bottom plates:

3 aspirate / dispense cycles, 300 µL/well, 96-tube manifold, final aspirate:
< 30 seconds

3 aspirate / dispense cycles, 100 µL/well, 96-tube manifold, final aspirate:
< 80 seconds
1 aspirate / dispense cycle, 400 µL/well, 192-tube manifold, final aspirate:
< 20 seconds
Filter bottom plates:
Variable, based on wash parameters
Fluid Delivery One internal positive displacement pump
Vacuum Filtration Selectable vacuum levels (approximate finals at 30 seconds):

Lowest: -38 mmHg
Low: -113 mmHg
Medium: -200 mmHg
High: -390 mmHg
Highest: -506 mmHg

Vacuum filtration time range:
5 to 999 seconds
Volume Range 25 to 3,000 µL/well
Selectable in 1 µL increments
Buffer Selection Internal automatic switching for up to 4 wash buffers (optional)
Flow Rates 1 to 11
Includes low flow, cell wash rates (model dependent)
Wash Cycles 1 to 250
Dispense Precision
  • 96-well washing - 96-tube (8 x 12) manifold:
    <3% CV: 300 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate 6, absorbance read at 630 nm and 450 nm reference
  • 384-well washing - 192-tube (16 x 12) manifold:
    < 4% CV: 80 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate 7, absorbance read at 630 nm and 450 nm reference
Residual Volume Solid bottom plates:

  • 96-well washing - 96-tube (8 x 12) manifold:
    2 µL/well: 3-cycle wash, 300 µL/well (deionized water with 0.1%
    Tween 20), Corning 96-well flat-bottom plate, aspiration height
    optimized prior to testing
  • 384-well washing - 192-tube (16 x 12) manifold:

Filter bottom plates:

  • 96-well:
    Average increase weight of plate < 1.2 g after dispensing 300 µL to 0.45 µm plate, filtration 30 seconds, low vacuum, blotted
  • 384-well:
    Average increase weight of plate < 4.0 g after dispensing 80 µL to 1.2
    µm plate, filtration 10 seconds, low vacuum, blotted
Sterilization Chemical
0.5% sodium hypochlorite, 70% isopropyl alcohol or 70% ethanol solution
Ultrasonic Cleaning Built-in ultrasonic bath for protein and salt crystal buildup
Programmable in hours and minutes, up to 60 minutes
(model dependent)
Clog Detection Built-in Verify technology detects aspirate and dispense tube blockage (model dependent)
Supply Bottle 4 L or 10 L (optional)
Physical Characteristics
Dimensions 14" W x 17" D x 10" H (35.6 x 43.2 x 25.4 cm)
Weight With internal buffer switching - 36 lbs (16.5 kg)
Without - 30 lbs (13.5 kg)
Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.

Specifications are subject to change. Performance values represent the average observed factory test values.


BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.

Liquid Handling Control™ Software

Liquid Handling Control™ Software

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.

Standard (Included)

Operators Manual

MANUAL Operator's manuals can be obtained via the Customer Resource Center


BioStack Integration Kits

7310040 BioStack Integration Kit for 405™ TS and 405™ LS

Bottles & Tubing

1173000 Dispense Bottle - with Filter, 10L accessory has picture
1173002 Cap for Dispense Bottle - with Filter, 10L
1173003 Cap for Dispense Bottle - with Filter, 4L accessory has picture
1173031 Dispense Bottle – with Filter, 4L accessory has picture
7100531 Cap for Waste Bottle, 4L (Also fits 10L or 20L) accessory has picture
7100533 Waste Tubing Set accessory has picture
7100534 Waste Bottle, 4L accessory has picture
7100537 Dispense Tubing Set – 4 Buffers
7100538 Dispense Tubing Set –1 Buffer accessory has picture
7100542 Waste Bottle with Level Sensing, 4L accessory has picture
7100543S Waste Set with Level Sensing, 4L Bottles accessory has picture
7100544 Cap for Waste Bottle with Level Sensing, 4L accessory has picture
7100556 Waste Bottle and Tubing, 20L accessory has picture
7100557 Waste Bottle and Tubing, 10L accessory has picture

Dispense/Waste Systems

1170528S Complete Dispense System, 10L Bottles accessory has picture
1170529S Complete Dispense System, 4L Bottles accessory has picture
1170530 Complete Dispense/Waste System 115V/230V, 4L Bottles accessory has picture
1170531S Complete High Flow Waste System 115V/230V, 10L Bottles
1170532 Complete Dispense/High Flow Waste System 115V/230V, 4L Bottles accessory has picture
1170533 Complete Dispense/High Flow Waste System 115V/230V, 10L Bottles
1170534S Complete Waste System 115V/230V, 10L Bottles
1170535 Complete Dispense/Waste System 115V/230V, 10L Bottles
1170554 Complete Dispense/Waste System 115V/230V, 20 L Bottles
1170555S Complete Waste System 115V/230V, 20L bottles
1170556 Complete Dispense/High Flow Waste System 115V/230V, 20L Bottles
1170557S Complete High Flow Waste System 115V/230V, 20L Bottles
7100582S Vacuum Set with Level Sense Assembly, 10L accessory has picture
7100746S Complete Waste System 115V/230V, 4L Bottles accessory has picture
7100753S Complete High Flow Waste System 115V/230V, 4L Bottles
7100754 High Flow Vacuum Pump 115/230V
7103024 Standard Vacuum Pump 115V/230V accessory has picture


01310 Filter for Dispense Bottle
48294 Filter for Waste Tubing accessory has picture


1170010 96-Tube Dual-Action Manifold Kit, with 70 dispense tubes and tube-in-tube aspiration manifold kit for 405 HT TS and 405 HT LS models only
1172046S 96-tube Dual-Action™ Tube-in-Tube Aspiration Manifold accessory has picture
7102068S 96-tube Dual-Action™ 7° Dispense Manifold


01113 Silencing Muffler for Standard Vacuum Pump
1170008 96-well Vacuum Filtration Kit accessory has picture
1170009 96-/384-well Vacuum Filtration Kit accessory has picture
1170013 15' extension kit for use with robotic system integrations of 405 TS / LS
4030551 Vacuum Gauge/Regulator
7100025S Stylus Kit for any 405™/ELx405™ Select Deep Well manifold accessory has picture
7102215 384-well Ring Magnet accessory has picture
7102216 96-well Ring Magnet accessory has picture
7103016 96-well Flat Magnet accessory has picture
7103017 384-well Flat Magnet accessory has picture
7210515 3-Instrument Rack – for third party robotic system integration accessory has picture

Product Qualification Packages

1170543 405™ LS Qualification Package - IQ/OQ/PQ package on CD

Test Plates & Reagents

1240001 Replacement 405 Verify test plate kit accessory has picture

Waste Systems

1170555S Complete Waste System 115V/230V, 20L Bottles
7100764S Waste set with Level Sensing, 20L Bottles

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