405 LS Microplate Washer

The 405™ LS Microplate Washer is the fifth generation BioTek washer, known for superior performance and reliability. Built-in automated buffer switching and "quick-change" manifold designs provide improved ease of use and efficiency when washing 96 and 384 well microplates.

Washer maintenance is critical for excellent assay performance, and the 405 LS now offers the new patented* Verify™ Technology for clog detection and patent-pending Ultrasonic Advantage™ for automated manifold cleaning, making the 405 LS a truly 'self-maintaining' system.

The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions. The optional BioStack™ is available for walk-away automation of up to 50 microplates.

Cell-based assays are easily processed with the 405 Select LS, with its angled dispense tubes, extensive aspirate and dispense rates and plate position controls providing optimal conditions for washing even fragile cell layers.  Magnetic separation and vacuum filtration modules are available for washing magnetic and polystyrene beads used in a growing number of multiplex assays and bead-based ELISAs, such as those developed on the Luminex® xMAP® technology platform. The vacuum filtration feature automates the wash steps of filter bottom plates with five different vacuum levels for optimal performance of filter pore sizes ranging from 0.45 µm to 1.2 µm. The 405 LS is Luminex® xMAP® approved.

A range of configurations for different microplate formats and assay requirements is available for microplate washing, each designed for optimal performance for your plate type and application.

* EP 1 637 887

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®

Hardware:

• Patented built-in Verify™ sensor controlled through Liquid Handling ControlTM (LHC) pc software, automatically checks dispense/aspirate performance and reports clogged tubes:

• Accommodates 96- and 384-well microplates
• 2x24 character backlit LCD display and membrane keypad user interface
• Patent-pending built-in Ultrasonic Advantage™ automatically cleans clogged tubes
• Automated internal 4-buffer switching
• Internal positive displacement pump eliminates use of troublesome pressure delivery systems and allows use of any size dispense reservoir
• Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates need for blank plates when used in automated robotic systems
• Quick change manifold design
• BioStack and 3rd party automation compatible
• All washers biomagnetic separation ready (accessory magnets sold separately)
• All washers vacuum filtration ready (accessory filtration modules sold separately)

405 Select LS

• 96-/384-well washing with patented 96-tube Dual-Action™ manifold
• Cell washing is optimized with two low-flow rates and 20° angled dispense tubes ideal for washing loosely adherent cell monolayers

405 HT LS

• Fast 384-well washing with patented 192-tube Dual-Action™ manifold for independent dispense and aspiration control for precise overfill washing and overflow protection:
• Superior tube-in-tube aspiration manifold design for 384-well washing with buffers not containing surfactant
• 96-well washing ready with optional 96-tube Dual-Action manifold

405 LS

• 405 LS models automate basic 96-well dispensing and aspiration

Software:

• Quick menu allows fluid paths to be easily primed, wash process started and manifold cleaned
• Predefined protocols included for out-of-box washing
• Fully customizable protocol parameters for individualized wash requirements - gentle cell-washing to vigorous wash for high-binding assays
• AutoPrime and auto maintenance routines
• Additional software flexibility and 21CFR Part 11 compliance with LHC™ Software for method development, downloading and washing plates
• Run protocols created onboard or downloaded from LHC Software

* With available accessory module sold separately

Specifications are subject to change.