Lionheart FX Automated Live Cell Imager

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Automated digital microscopy up to 100x

Automated digital microscopy up to 100x

Brightfield, color brightfield, phase contrast and fluorescence imaging

Brightfield, color brightfield, phase contrast and fluorescence imaging

Live cell assay support with environmental controls

Live cell assay support with environmental controls

Compact design for quick installation and setup

Compact design for quick installation and setup

Lionheart™ FX Automated Live Cell Imager with Augmented Microscopy™ is an inclusive microscopy system. Brightfield, color brightfield, phase contrast and fluorescence channels offer maximum support for a wide range of imaging applications. The system is optimized for live cell imaging with temperature and CO2/O2 control, plus an available humidity chamber and reagent injector to add greater optimization for live cell imaging under ideal physiological conditions.

Gen5™ 3.0  software provides automated image capture, processing and analysis, plus annotation and movie maker functions. Capture and analyze images across a broad range of live and fixed cell applications, including perfusion assays. Augmented Microscopy is the combination of all of these features in one compact system. With Lionheart FX, you can capture, process, analyze, and generate publication-ready images and data with ease. .
 

www.cellimager.com

 

Visit BioTek’s Imaging & Microscopy microsite for details of the wide range of applications, image processing and analysis capabilities available with Lionheart FX Automated Live Cell Imager and Cytation Cell Imaging Multi-Mode Readers, along with Gen5 3.0 Software.

Automated digital microscopy to 100x

Automated digital microscopy to 100x
  • Automated image capture, automated image analysis, automate movie making
  • Image-based and laser autofocus, auto LED intensity, auto exposure
  • High contrast brightfield for label-free cell counting
  • Image whole organisms to subcellular details


Four imaging modes, multiple imaging processes

Four imaging modes, multiple imaging processes
  • Brightfield, color brightfield, phase contrast and fluorescence
  • Z-stacking and z-projection, montage and stitching
  • Digital phase contrast


Gen5 3.0 Software tools

Gen5 Software tools
  • Integrated image capture, analysis, annotation and movie making on a single platform
  • Quick implementation, no extensive training required
  • Gen5 Image+ and Gen5 Image Prime for expanded image analysis


Kinetic live cell assay support

Kinetic live cell assay support
  • Environmental control cover contains temperature and gas
  • Humidity chamber supports long term live cell kinetics
  • Dual reagent injectors provide quick inject/image to observe fast reactions


Fully integrated and compact

Lionheart FX
  • Thoughtful design offers quick installation and setup
  • Tool-free connections for gas controller, injectors and environmental control cover
  • Filter cubes and objectives easily accessible from the front panel

 

LFX
Lionheart FX Automated Live Cell Imager. Fluorescence, brightfield, color brightfield and phase contrast imaging capabililty. Includes base Gen5 software. Objectives, filter/LED cubes, accessories and software upgrades sold separately.

General
Microplate types 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Support for labware up to 1.5" tall
Temperature control Incubation to 40 °C with optional environmental control cover
Software Gen5 Microplate Reader and Imager Software included
Gen5 Image and Image+ Software available for advanced image analysis (option)
CO2 and O2 control (option) 0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller
Environmental control cover Top cover for light tight imaging and incubation control (option)
X/Y Stage resolution Lead screw driven stage with 0.1 micron resolution
Humidity control Humidity chamber with rapid gas recharge (option)
Imaging System
Imaging mode Fluorescence, brightfield, color brightfield, and phase contrast
Imaging method Single color, multi-color, montage, time lapse, Z-stacking, burst mode
Light source High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Camera 16-bit gray scale, Sony CCD, 1.25 megapixel
Camera binning Optional 2x2 binning for focus and/or image capture
Camera exposure range 5 milliseconds to 4 seconds
Image outputs available Raw Images: 16-bit TIFF
Saved Images: TIF, JPG, BMP, PNG, EMF, GIF
Movies: MP4, WMV
Objective capacity 6 onboard, user-replaceable objectives
Objectives available Fluorescence:
Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30;
20x, NA: .45; 40x, NA.60; 60x, NA .70
Oil: 60x, NA:1.42; 100x, NA: 1.40
Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method Image-based autofocus
Laser autofocus option
Image collection rate Single well fastest frame rate capture:
*Full Resolution: up to 10 frames per second for single color images
*2x2 Binning: up to 20 frames per second for single color images
Microscope stage control Gen5 Software Control
Optional joystick controller
Reagent Injectors (option)
Number 2 syringe pumps
Supported labware 6- to 384-well microplates, Petri and cell culture dishes, chamber slides
Dead volume <1.65 mL with back flush
Dispense tip options Aligned tip - aligned with optical path for dispensing for fast kinetic assays
Offset tip - dispensing is offset from the optical path
Dispense volume 5 - 1000 µL in 1 µL increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL
Physical Characteristics
Power 250 Watts max.
Dimensions With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm)
Weight Without environmental control cover: 51 lbs (23.1 kg)
With environmental control cover: 58 lbs (26.3 kg)
Regulatory
Regulatory CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Microplate Reader and Imager Software

Gen5 Microplate Reader and Imager Software

BioTek’s Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.
Note: Some instruments come with a specific edition of Gen5, please check the microplate reader specifications.


Optional

Adapters and Labware Holders

1220548 Slide adapter accessory has picture
1320016 Counting Chamber Adapter accessory has picture
1450006 Humidity chamber accessory has picture
1450007 T75 stage insert accessory has picture
1450008 T75 u-shaped flask stage insert accessory has picture
1450527 Spare microplate/slide stage insert accessory has picture
1450541 Multi-vessel adapter accessory has picture
1452275 T-25 adapter accessory has picture
1530006 Stage Insert CellASIC ONIX2 Basic accessory has picture
1530007 Stage Insert CellASIC ONIX2 XT accessory has picture

Imaging Filter Cubes

1225100 DAPI Filter cube accessory has picture
1225101 GFP Filter cube accessory has picture
1225102 Texas Red Filter cube accessory has picture
1225103 RFP Filter cube accessory has picture
1225104 YFP Filter cube accessory has picture
1225105 CY5 Filter cube accessory has picture
1225106 CY7 Filter cube accessory has picture
1225107 CFP Filter cube accessory has picture
1225109 Acridine Orange (+RNA) filter cube accessory has picture
1225111 Propidium Iodide filter cube accessory has picture
1225112 Chlorophyll A filter cube accessory has picture
1225113 469/593 filter cube accessory has picture
1225114 Cy5.5 filter cube accessory has picture
1225115 TagBFP filter cube accessory has picture
1225116 GFP excitation - CY5 emission
1225117 RFP excitation - CY5 emission
1225118 CFP FRET V2 filter cube
1225119 CFP/YFP FRET V2 filter cube
1225121 Ex 377 Em 647 filter cube

Imaging LED Cubes

1225001 465 nm LED cube accessory has picture
1225002 590 nm LED cube accessory has picture
1225003 523 nm LED cube accessory has picture
1225004 505 nm LED cube accessory has picture
1225005 623 nm LED cube accessory has picture
1225006 740 nm LED cube accessory has picture
1225007 365 nm LED cube
1225008 655nm LED cube
1225009 390nm LED cube
1225011 405 nm LED cube

Imaging Objectives

1220517 20x objective, Plan Fluorite WD 6.6 NA 0.45
1220518 10x objective, Plan Fluorite WD 10 NA 0.3
1220519 4x objective, Plan Fluorite WD 17 NA 0.13
1220539 2.5x objective (2.75x eff.) Zeiss, Fluorite WD 6.3 N.A: 0.12
1220544 40x objective, Plan Fluorite WD 2.7 NA 0.6
1220545 60x objective, Plan Fluorite WD 1.8 NA 0.7
1220549 2.5x objective (2.25x eff.) Meiji, Plan Achromat, WD 6.2 NA: 0.07
1220562 1.25x objective, Plan Apochromat WD 5 NA 0.04
1320515 4x phase objective, Plan Fluorite WD 17 NA 0.13
1320516 10x phase objective, Plan Fluorite WD 10 NA 0.3
1320517 20x phase objective, Plan Fluorite WD 6.7 NA 0.45
1320518 40x phase objective, Plan Fluorite WD 2.7 NA 0.6 accessory has picture
1320520S Spare Phase Contrast Annulus for 4x Objective
1320521S Spare Phase Contrast Annulus for 10x / 20x Objective
1322026 Spare Phase Contrast Annulus for 40x Objective
1322500 Spare Alignment Tool for 10x / 20x Phase Contrast Objectives
1322507 Spare Alignment Tool for 40x Phase Contrast Objectives
1450538 60x oil objective, Plan Apochromat WD 0.15 NA 1.42 accessory has picture
1450539 100x oil objective, Plan Apochromat WD 0.13 NA 1.4 accessory has picture

Miscellaneous

01985 Spare Cover Slip for Counting Chamber
02221 Spare oil for 60x and 100x oil objectives
1210012 CO2 - only gas controller accessory has picture
1210013 CO2 and O2 gas controller accessory has picture
1220024 Laser autofocus retrofit kit for field installed Cytation and Lionheart FX
1225010 Laser autofocus cube for new Lionheart FX and Cytation imaging (“V”) configurations accessory has picture
1320003 Joystick accessory has picture
1320023 High Contrast Brightfield Kit for Label-Free Cell Counting
1322131 Counting Chamber accessory has picture
1450009 Environment Control Cover

Reagent Injector Assembly

1450532 Injector tip – offset accessory has picture
1450533 Dispense tip aligned accessory has picture
1452124 Injector tubing bracket
8040031 Dual reagent injector module for use with Lionheart FX accessory has picture

Software Options

GEN5IPLUS Gen5 Image+ accessory has picture
GEN5SECUREIPLUS Gen5 Secure Image+ accessory has picture
Gen5IPRIME Gen5 Image Prime
Gen5SECUREIPRIME Gen5 Secure Image Prime

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