Cytation 3 Cell Imaging Multi-Mode Reader

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Automated digital microscope and multi-mode reader in one

Modular and upgradable: buy only what you need now

2.5x to 60x imaging: population analysis, intra-cellular details

Award Winning Technology

Cytation 3 Image Catalog

Cytation™ 3 is a cell imaging multi-mode microplate reader that combines automated digital microscopy and conventional microplate detection.  This unique patent pending design provides rich phenotypic cellular information with well-based quantitative data. Equipped with BioTek’s patented Hybrid Technology™ for microplate detection, Cytation 3 includes both high sensitivity filter-based detection and a flexible quadruple monochromator based system for unmatched versatility and performance. The upgradable fluorescence cellular imaging module, with up to 60x magnification provides researchers rich cellular visualization analysis without the complexity and expense of standard automated microscopes, making cell imaging more accessible to a larger number of research laboratories at an affordable price.

Fluorescence microscopy is a powerful technique for visualizing cellular responses to understand cell proliferation, protein expression, cytotoxicity and other expressions. The ability to perform both conventional quantitative fluorescence measurements and cell imaging takes cell biology research to another level.  With special emphasis on live-cell assays, Cytation 3 features temperature control to 45 °C, CO2/O2 gas control, orbital shaking and full support for time lapse studies with BioTek’s Gen5™ software, specifically designed to make plate reading and image capture easy.

Technology advances are found throughout Cytation 3’s including high-intensity LED light sources, filter cubes optimized for high signal/noise ratio, hard coated optical filters, Olympus objectives, and superior autofocus for totally software controlled microscopy.

Cytation 3 is the recipient of the 2013 SelectScience Drug Discovery Product of the Year Award, 2013 Miptec New Product Innovation Award, Thermo Fisher Scientific “Extraordinary New Product Line Award” and  2014 Silver Edison Award.

 

Images captured with Cytation 3
View the Cytation 3 Image Catalog for additional images.

Images captured with Cytation 3: 4x zebrafish, 3T3 cells 20x, BPAE cell 60x, Z-stacked tumoroid 20x, CFP yeast brightfield 40x and c.elegans 20x



Cytation 3 Video


US Federal Government customers:
This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note GSA Contract in the Comments field).

Cytation 3 Image Catalog
  • Automated digital microscopy and/or multi-mode microplate detection in one instrument
  • Modular and upgradable: microscopy only, microplate reading only, or both
  • Flexible sample format: 6- to 384-well plates, microscope slides, cell culture flasks and Petri dishes
  • Affordable automation: automated XY stage, auto focus, auto exposure, automated image capture, LED intensity
  • Inverted fluorescence and brightfield microscopy
  • High Power LED cubes that provide superior excitation energy and long life
  • Optimized epifluorescence filter cubes for superior sensitivity for commonly used fluorophores: DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5 and CY7
  • From 2.5x for full-well imaging to 60x for sub-micron resolution for intra-cellular details
  • High quality images: Olympus objectives, 16-bit gray scale CCD camera, Semrock filters.
  • Live cell assays with temperature control up to 45 °C, CO2/O2 gas control accessory
  • Endpoint, time-lapse and montage assays
  • Automatic cell counting, sub-population analysis and image statistics
  • Dual reagent injectors
  • Patented Hybrid Technology™ microplate reading mode with high performance filter-based optics and high-flexibility quadruple monochromator optics
Part #
CYT3MFV
CYT3MV
CYT3FV
CYT3V
CYT3MF
CYT3M
CYT3F
Fluorescence and brightfield imaging
     
Monochromator fluorescence
 
 
 
Monochromator absorbance
   
 
Filter/dichroic fluorescence
 
 
 
Fluorescence polarization
 
 
 
Time resolved fluorescence
•*
 
•*
Luminescence
 
Filtered luminescence
 
 
 
Temperature control to 45°C
Linear, orbital, double-orbital shaking
Gas controller compatible**
Dual reagent injector compatible**
Gen5 Data Analysis software
* TRF is secondary mode in monochromator-only configurations
**Gas controller module and dual reagent injector module sold separately.

Non-"V" configurations can be upgraded to add fluorescence and brightfield imaging
"V" configurations
can be upgraded to add monochromator and/or filter-based multi-mode detection

 

General
Imaging mode Fluorescence and brightfield
Detection mode Monochromators: FL, Lum, UV-Vis Abs
Filters: FL, TRF, FP, Lum
Read method Endpoint, time-lapse, kinetic, well mode, montage
Labware type 6- to 384- well microplates, microscope slides, cell culture flasks (T25), 35 mm, 60 mm, 100 mm Petri dishes
Compatible with Take3™ Micro-Volume Plates with 2 µL microspots
Temperature control To 45 °C +0.2 °C at 37 °C
Independent top and bottom temperature control
Shaking Linear, orbital, double orbital
Automation BioStack and 3rd party automation compatible
CO2 and O2 Control 0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller
Software Gen5™ Data Analysis Software or Gen5 Image+ (optional)
Imaging System
Camera 16-bit, gray scale, Sony CCD, 1.1 megapixel
Imaging filter cube capacity 4 onboard, user-replaceable filter cubes
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange (ACR OR), CFP-YFP FRET, and Propidium Iodide
Imaging LED cubes available 365 nm, 465 nm, 590 nm, 523 nm, 505 nm, 623 nm, 740 nm
Objective capacity 2 onboard user-replaceable objectives
Available objectives 2.5x (2.25x eff), 2.5x Zeiss(2.75 eff), 4x, 10x, 20x, 40x, 60x
Image collection rate 96 wells, 1 color (DAPI), 4x, 6 minutes
96 wells, 3 colors, 4x, 12 minutes
Fluorescence Intensity
Wavelength Selection Quadruple monochromators (top and bottom) and, deep blocking bandpass filters / dichroic mirrors (top)
Sensitivity Monochromators:
Top: fluorescein 2.5 pM (0.25 fmol/well 384-well plate)
Bottom: fluorescein 4 pM (0.4 fmol/well 384-well plate)

Filters/mirrors:
Fluorescein 0.25 pM (0.025 fmol/well 384-well plate)
Light Source Xenon Flash lamp
Wavelength Range Monos: 250 - 700 nm (850 option)
Filters: 200 - 700 nm (850 option)
Dynamic Range 5 decades
Detection System Two PMT detectors: one for monochromator system, one for filter system
Luminescence
Sensitivity Monochromator system: 20 amol ATP (flash)
Filter system: 10 amol ATP (flash)
100 amol ATP (glow)
Wavelength Range 300 - 700 nm
Dynamic Range >6 decades
Fluorescence Polarization
Sensitivity 1.2 mP standard deviation at 1 nM fluorescein
Wavelength Range 320 - 700 nm (850 nm option)
Time-Resolved Fluorescence
Sensitivity Europium 40 fM with filters (4 amol/well in 384-well plate)
Europium 1200 fM with monos (120 amol/well in 384-well plate)
Light Source Xenon flash lamp
Wavelength Range Monos: 250 - 700 nm
Filters: 200 - 700 nm (850 option)
Absorbance
Light Source Xenon flash lamp
Wavelength Selection Monochromator
Wavelength Range 230 - 999 nm, 1 nm increment
Bandpass 4 nm (230-285 nm), 8 nm (>285 nm)
Dynamic Range 0 - 4.0 OD
Resolution 0.0001 OD
Reagent Dispensers
Number 2 syringe pumps
Dispense Volume 5 - 1000 µL in 1 µL increment
Dead Volume 1 mL, 100 µL with back flush
Plate Geometry 6- to 384-well microplates
Dispense Precision <2% at 50-200 µL
Dispense Accuracy +1 µL or 2%
Physical Characteristics
Power 130 Watts max.
Dimensions 20" D x 16.5" W x 17.5" H
(50.8 cm x 41.91 cm x 44.5 cm)
Weight 80 lbs (36.3 Kg)
Regulatory
Regulatory CE and TUV marked. Models for In Vitro Diagnostic use are available.

Specifications are subject to change. Performance values represent the average observed factory test values.

Standard (Included)

Gen5 Data Analysis Software

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.


Optional

BioStack Microplate Stacker

BioStack Microplate Stacker

BioStack™ is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. BioStack offers rapid plate exchange speeds to increase throughput and enhance productivity, accommodating assay workflows both with and without plate lids.


Optional

Adapters

1220548 Slide holder adapter accessory has picture
1222210 T25 cell culture flask adapter accessory has picture
1222240 Petri dish adapter, 2x 35 mm dish accessory has picture
1222241 Petri dish adapter, 60 mm dish accessory has picture
1222242 Petri dish adapter, 100 mm dish accessory has picture

BioStack Integration Kits

7310053 BioStack Integration Kit for Cytation 3

Bottles & Tubing

1210011 Calibration Kit for CO2 Controller

Filter Accessories

7082073 Plug (Dummy filter) for Synergys and FLx800 accessory has picture
7082075 Retainer Clip for Synergys and FLx800 accessory has picture

Filter Cubes

8040501S Filter Cube: Blue (360/460) / Green (485/528) accessory has picture
8040502 Filter Cube: Green (485/528) / Red (530 / 590) accessory has picture
8040505S Filter Cube: HTRF® – TR-FRET accessory has picture
8040506 Filter Cube: LanthaScreen – TR-FRET accessory has picture
8040507 Filter Cube: Lance – TR-FRET accessory has picture
8040553S Filter Cube – Luminescence accessory has picture
8040561 Filter Cube: Green FP (485/528) accessory has picture
8040562 Filter Cube: Red FP (530 / 590) accessory has picture
8040563 Blue/UV FP filter set: 360/40 EX, 460/40 EM, 400 nm mirror, UV polarizers
8040564 Empty Filter Cube - Fluorescence Polarization Visible (excitation > 400nm)
8040565 Empty Filter Cube - Fluorescence Polarization UV (excitation between 300 and 400 nm)
8040566 Empty Filter Cube - non FP applications

Filters

7092202 274/5 nm Fluorescence Filter accessory has picture
7092146 276/10 nm Fluorescence Filter accessory has picture
7092145 276/5 nm Fluorescence Filter accessory has picture
7092148 280/10 nm Fluorescence Filter accessory has picture
7092147 280/5 nm Fluorescence Filter accessory has picture
7082259 284/10 nm Fluorescence Filter accessory has picture
7092149 284/5 nm Fluorescence Filter accessory has picture
7082248 310/20 nm Fluorescence Filter accessory has picture
7082250 320/20 nm Fluorescence Filter accessory has picture
7092205 325/50 nm Fluorescence Filter accessory has picture
7092150 330/20 nm Fluorescence Filter accessory has picture
7082263 330/80 nm Fluorescence Filter accessory has picture
7082254 340/11 nm Fluorescence Filter accessory has picture
7082230 340/30 nm Fluorescence Filter accessory has picture
7092151 355/20 nm Fluorescence Filter accessory has picture
7082220 360/40 nm Fluorescence Filter accessory has picture
7092152 380/11 nm Fluorescence Filter accessory has picture
7082228 380/20 nm Fluorescence Filter accessory has picture
7092153 395/25 nm Fluorescence Filter accessory has picture
7082242 400/10 nm Fluorescence Filter accessory has picture
7082205 400/30 nm Fluorescence Filter accessory has picture
7092154 405/20 nm Fluorescence Filter accessory has picture
7092155 410/10 nm Fluorescence Filter accessory has picture
7092200 410/80 nm Fluorescence Filter accessory has picture
7082270 420/27 nm Fluorescence Filter accessory has picture
7082206 420/50 nm Fluorescence Filter accessory has picture
7082227 440/30 nm Fluorescence Filter accessory has picture
7082207 440/40 nm Fluorescence Filter accessory has picture
7082208 450/50 nm Fluorescence Filter accessory has picture
7082222 460/40 nm Fluorescence Filter accessory has picture
7092157 470/40 nm Fluorescence Filter accessory has picture
7092158 475/20 nm Fluorescence Filter accessory has picture
7082221 485/20 nm Fluorescence Filter accessory has picture
7082209 485/40 nm Fluorescence Filter accessory has picture
7092160 490/10 nm Fluorescence Filter accessory has picture
7082268 495/10 nm Fluorescence Filter accessory has picture
7092162 500/20 nm Fluorescence Filter accessory has picture
7082256 500/27 nm Fluorescence Filter accessory has picture
7082218 508/20 nm Fluorescence Filter accessory has picture
7092201 515/30 nm Fluorescence Filter accessory has picture
7082246 516/20 nm Fluorescence Filter accessory has picture
7092163 520/10 nm Fluorescence Filter accessory has picture
7092164 520/20 nm Fluorescence Filter accessory has picture
7082247 528/20 nm Fluorescence Filter accessory has picture
7082223 530/25 nm Fluorescence Filter accessory has picture
7092166 530/30 nm Fluorescence Filter accessory has picture
7092167 532/40 nm Fluorescence Filter accessory has picture
7092168 540/15 nm Fluorescence Filter accessory has picture
7082249 540/25 nm Fluorescence Filter accessory has picture
7082253 540/35 nm Fluorescence Filter accessory has picture
7092169 540/45 nm Fluorescence Filter accessory has picture
7082210 545/40 nm Fluorescence Filter accessory has picture
7092170 550/10 nm Fluorescence Filter accessory has picture
7092171 556/10 nm Fluorescence Filter accessory has picture
7092172 560/10 nm Fluorescence Filter accessory has picture
7082215 560/15 nm Fluorescence Filter accessory has picture
7082211 560/20 nm Fluorescence Filter accessory has picture
7082212 560/40 nm Fluorescence Filter accessory has picture
7082271 565/10 nm Fluorescence Filter
7092173 570/10 nm Fluorescence Filter accessory has picture
7092174 575/10 nm Fluorescence Filter accessory has picture
7082245 575/15 nm Fluorescence Filter accessory has picture
7092175 580/10 nm Fluorescence Filter accessory has picture
7082244 580/50 nm Fluorescence Filter accessory has picture
7092176 585/10 nm Fluorescence Filter accessory has picture
7092177 590/10 nm Fluorescence Filter accessory has picture
7082225 590/20 nm Fluorescence Filter accessory has picture
7082224 590/35 nm Fluorescence Filter accessory has picture
7092178 596/15 nm Fluorescence Filter accessory has picture
7082252 600/40 nm Fluorescence Filter accessory has picture
7092179 610/10 nm Fluorescence Filter accessory has picture
7082265 620/10 nm Fluorescence Filter accessory has picture
7082251 620/15 nm Fluorescence Filter accessory has picture
7082213 620/40 nm Fluorescence Filter accessory has picture
7092180 625/10 nm Fluorescence Filter accessory has picture
7082214 635/32 nm Fluorescence Filter accessory has picture
7092182 645/10 nm Fluorescence Filter accessory has picture
7082226 645/40 nm Fluorescence Filter accessory has picture
7092183 660/10 nm Fluorescence Filter accessory has picture
7092184 665/34 nm Fluorescence Filter accessory has picture
7082266 665/8 nm Fluorescence Filter accessory has picture
7092185 670/10 nm Fluorescence Filter accessory has picture
7092186 670/20 nm Fluorescence Filter accessory has picture
7092187 680/10 nm Fluorescence Filter accessory has picture
7092188 680/20 nm Fluorescence Filter accessory has picture
7082229 680/30 nm Fluorescence Filter accessory has picture
7092189 690/10 nm Fluorescence Filter accessory has picture
7092190 700/20 nm Fluorescence Filter accessory has picture
7092191 710/20 nm Fluorescence Filter accessory has picture
7092192 730/40 nm Fluorescence Filter accessory has picture
7092193 760/20 nm Fluorescence Filter accessory has picture
7092194 760/34 nm Fluorescence Filter accessory has picture
7092195 781/20 nm Fluorescence Filter accessory has picture
7092196 785/20 nm Fluorescence Filter accessory has picture
7092197 807/25 nm Fluorescence Filter accessory has picture
7092198 818/40 nm Fluorescence Filter accessory has picture

Imaging Filter Cubes

1225100 DAPI Filter cube accessory has picture
1225101 GFP Filter cube accessory has picture
1225102 Texas Red Filter cube accessory has picture
1225103 RFP Filter cube accessory has picture
1225104 YFP Filter cube accessory has picture
1225105 CY5 Filter cube accessory has picture
1225106 CY7 Filter cube accessory has picture
1225107 CFP Filter cube accessory has picture
1225109 Acridine Orange (ACR OR) filter cube
1225110 CFP-YFP FRET filter cube
1225111 Propidium Iodide filter cube

Imaging LED Cubes

1225000 365 nm LED cube accessory has picture
1225001 465 nm LED cube accessory has picture
1225002 590 nm LED cube accessory has picture
1225003 523 nm LED cube accessory has picture
1225004 505 nm LED cube accessory has picture
1225005 623 nm LED cube accessory has picture
1225006 740 nm LED cube accessory has picture

Imaging Objectives

1220517 Objective 20x mag
1220518 Objective 10x mag
1220519 Objective 4x mag
1220539 Objective 2.5x mag Zeiss (2.75x eff.)
1220544 Objective 40x mag (Requires Gen5 Image+)
1220545 Objective 60x mag (Requires Gen5 Image+)
1220549 Objective 2.5x mag (2.25x eff.)

Mirrors

7132121 Half-size dichroic mirror, 50%, Ex 200-850 nm, Em 200-850 nm accessory has picture
7137400 Full-Size Dichroic Mirror, Ex. 320-390, Em. 410-800 accessory has picture
7137435 Full-Size Dichroic Mirror, Ex. 385-425, Em. 445-610 accessory has picture
7137455 Full-Size Dichroic Mirror, Ex. 400-450, Em. 460-710 accessory has picture
7137510 Full-Size Dichroic Mirror, Ex. 440-505, Em. 515-640 accessory has picture
7137525 Full-Size Dichroic Mirror, Ex. 475-520, Em. 530-670 accessory has picture
7137545 Full-Size Dichroic Mirror, Ex. 512-535, Em. 555-578 accessory has picture
7137550 Full-Size Dichroic Mirror, Ex. 415-540, Em. 560-850 accessory has picture
7137555 Full-Size Dichroic Mirror, Ex. 541-550, Em. 560-595 accessory has picture
7137570 Full-Size Dichroic Mirror, Ex. 515-565, Em. 575-735 accessory has picture
7137595 Full-Size Dichroic Mirror, Ex. 540-590, Em. 600-770 accessory has picture
7138320 Half-Size Dichroic Mirror, Ex. 260-305, Em. 335-750 accessory has picture
7138365 Half-Size Dichroic Mirror, Ex. 290-350, Em. 380-800 accessory has picture
7138400 Half-Size Dichroic Mirror, Ex. 320-390, Em. 410-800 accessory has picture
7138435 Half-Size Dichroic Mirror, Ex. 385-425, Em. 445-610 accessory has picture
7138455 Half-Size Dichroic Mirror, Ex. 400-450, Em. 460-710 accessory has picture
7138510 Half-Size Dichroic Mirror, Ex. 440-505, Em. 515-640 accessory has picture
7138525 Half-Size Dichroic Mirror, Ex. 475-520, Em. 530-670 accessory has picture
7138545 Half-Size Dichroic Mirror, Ex. 512-535, Em. 555-578 accessory has picture
7138550 Half-Size Dichroic Mirror, Ex. 415-540, Em. 560-850 accessory has picture
7138555 Half-Size Dichroic Mirror, Ex. 541-550, Em. 560-595 accessory has picture
7138570 Half-Size Dichroic Mirror, Ex. 515-565, Em. 575-735 accessory has picture
7138595 Half-Size Dichroic Mirror, Ex. 540-590, Em. 600-770 accessory has picture
7138660 Half-Size Dichroic Mirror, Ex. 580-655, Em. 665-850 accessory has picture

Miscellaneous

01604 FireWire Interface 1394B for desktop computers
1210007 Gas Controller for CO2 control. Includes CO2 flow meter & tubing set (p/n 1213032) accessory has picture
1210008 Gas Controller for CO2 and O2 control. Includes CO2 and N2 flow meters & tubing set (p/n 1213033 and p/n 1213032). accessory has picture
1220521 Anti-vibration plate
1220535 FireWire Laptop Card/Supply for laptop computers

Product Qualification Packages

1220514 Cytation 3 Product Qualification Package
1222520 Jig Assembly Tilt and Contrast (Autofocus)
1222531 40x/60x Setup Plate

Reagent Injector Assembly

8040003S Dual Reagent Dispenser Module option accessory has picture

Reagent Injector Options

1320514 Angled tip injector head accessory has picture

Software Options

GEN5SECURE Gen5 Secure accessory has picture
GEN5IVD Gen5 IVD accessory has picture
GEN5SECUREIPLUS Gen5 Secure Image+ accessory has picture
GEN5IVDIPLUS Gen5 IVD Image+ accessory has picture
GEN5IPLUS Gen5 Image+ accessory has picture

Take3 Micro-Volume Plate

TAKE3 Take3 Micro-Volume Plate accessory has picture
Take3Trio Take3 Trio Micro-Volume Plate accessory has picture

Test Plates & Reagents

7092092 Fluorescence Test Plate accessory has picture
7260522E Absorbance Test Plate accessory has picture
8030015 Luminescence Test Plate accessory has picture

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