BioSpa Live Cell Imaging System

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Full unattended workflow automation for up to 8 microplates /labware

Full unattended workflow automation for up to 8 microplates /labware

Integrated temperature and CO2/O2 control and monitoring, plus humidity control

Integrated temperature and CO2/O/2/ control and monitoring, plus humidity control

Fluorescence, brightfield, color brightfield and phase contrast

Fluorescence, brightfield, color brightfield and phase contrast

Washing, media replacement and reagent dispensing

Washing, media replacement and reagent dispensing

The BioSpa Live Cell Imaging System brings together BioSpa Automated Incubator and Cytation 5 Cell Imaging Multi-Mode Reader to fully automate live cell imaging and analysis. Plate washing and reagent dispensing can be added to the system to automate the entire process from sample prep through image analysis. Cytation 5 provides high quality, powerful data-rich image capture, processing and analysis of live cell assays, up to 60x. BioSpa's temperature, gas and humidity controlled environment houses and protects cells in up to 8 microplates or other labware. The robotic gripper moves the plate from the incubator drawers to Cytation 5 for kinetic imaging and analysis in Gen5 software.  BioSpa’s software seamlessly integrates imaging and liquid handling protocols in an easy scheduling interface - reporting and 24/7 monitoring of all processes.

HT-1080 Wound Healing 10s     Confluency Graph

BioSpa System automates a wide range of live cell imaging applications, including scratch wound healing

  • Full unattended workflow automation for up to 8 plates or other labware
  • Real time temperature and CO2/O2 control and monitoring, plus humidity monitoring
  • Continuous recording of environment conditions and customized notifications
  • Fluorescence, brightfield, color brightfield and phase contrast imaging modes
  • Up to 60x magnification and up to 4 color channels covers a range of applications
  • Single and multi-color, montage, time lapse and automated z-stacking
  • Z-projection, digital phase contrast and image stitching for powerful processing
  • Image-based and available laser autofocus: fast and accurate for a variety of applications
  • Washing, media replacement and dispensing to automated sample prep through imaging
  • Gen5 3.0 Software with image capture, analysis, annotation and movie maker
  • Compact system integration fits in biosafety cabinet, providing the ultimate cell assay protection
Session Chart
Real time temperature and CO2/O2 control and monitoring, plus humidity monitoring

BioSpa Live Cell Imaging system can be comprised of of BioSpa 8 Automated Incubator, along with other compatible imagers, washers and dispensers:



BioSpa System

 

General
Microplate types 6- to 1536-well standard height microplates, with or without lids.
Plate height range: 7.6 mm to 25.4 mm
Other labware supported Cell culture dishes (60 mm and 35 mm), T25 flasks
Lidded plate handling Robotic arm moves plate to and from BioSpa 8 and connected instrument; handles de-lidding and re-lidding plates.
Microplate capacity Up to 8 microplates (or other labware)
Air filter User-replaceable HEPA filter
Compatible instruments Cytation 5, Cytation 3, Synergy Neo2, Synergy H1, Epoch 2, EL406, 405 TS, 405 LS, MultiFlo FX, MultiFlo
Temperature control Range: to 45 °C
Control resolution: 0.1 °C
Uniformity + 0.5 °C at 37 °C
Software BioSpa Automated Incubator Software:

Provides programming interface for BioTek detection and liquid handling devices
Allows user notification (text or email) of events and/or errors in the system
Provides control, monitoring and logging of:
    CO2/O2
    incubator temperature
Provides humidity level monitoring and logging
Allows long-term uninterrupted runs up to 2 weeks
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: +0.1% (CO2 and O2)
Stability: +0.2% at 5% CO2+0.2% at 1% O2
Humidity control rH: 80 to 95% (lidded plates and 5% CO2)
Source: Removable water pan
Water level sensor: Low water level alert
Imaging System
Imaging mode Fluorescence
Brightfield
Color Brightfield
Phase Contrast
Imaging method Single color, multi-color, montage, time lapse, z-stacking
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit gray scale, Sony CCD, 1.25 megapixel. 0.3 µm/pixel at 20x
Objective capacity 6 user-replaceable objectives
Objectives available 1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x
Phase objectives available 4x, 10x, 20x, 40x
Image filter cube capacity 4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions User-trained autofocus, autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Liquid handling See the individual product pages at EL406, MultiFlo FX and 405 TS

Specifications are subject to change. Performance values represent the average observed factory test values.


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