Patent PendingDual-Action™ Manifold

BioTek’s dual-action manifold design (US Patent 5,957,783) allows independent control and positioning of both the aspirate and dispense sections of the manifold, providing three major benefits:

  1. Rapid washing of either 96- or 384-well plates on one platform
  2. Ideal positioning for gentle washing of sensitive cell-based assays and
  3. Overfill/overflow washing with continuous fill and aspiration at the tops of the wells, vigorous washing for ELISA assays.

The dual-action manifold allows independent control of dispense and aspirate tubes for optimal x-, y- and z- positions.

BioTek's dual-action manifold allows independent control of dispense and aspirate tubes for optimal x-, y- and z- positions. Dispense tubes are angled to 20° for gentle dispensing into well containing delicate cell layers.


BioTek’s 405 Microplate Washer, EL406 Washer Dispenser, MultiFlo FX Multi-Mode Dispenser and ELx50 Strip Plate Washer all incorporate the dual-action manifold in standard or optional configurations. As cell-based assays become more prevalent, the benefits of the dual-action manifolds with their angled tubes are more important. The angled dispense tubes can be positioned to provide a gentle flow down the sides of a 96- or 384-well plate so as not to disrupt the cell layer.

Top photos: cell layer before and after dispense with angled tubes. Bottom photos: cell layer before and after dispense with straight tubes

The photo above shows a) cell layer before dispense and b) after dispense with angled tubes. Photos c) show a cell layer before and d) after dispense with straight tubes

Dual-Action Manifold Benefits:

  • Wash 96- and 384-well microplates on one instrument without changing the manifold
  • Optimal positioning of aspirate tubes for more or less residual after dispense
  • Optimal positioning of dispense tubes for cell-based assays or for standard washing
  • Angled dispense tubes won’t disrupt cell layers
  • Allows vigorous overfill/overflow washing


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