Application Notes | Technical Resources : BioTek

Absorbance Detection

Analysis of Histamine in Wine Samples Using the Microplate Format (2006-08-29)

BioTek PowerWave 200 Microplate Spectrophotometer: Nitric Oxide Synthesis (2001-02-07)

BioTek PowerWave HT Microplate Spectrophotometer: Quantitation of Nucleic Acids (2001-02-06)

Determination of ATPase Activity Using BioTek's ELx808 Microplate Reader (2001-09-24)

Determination of Total Protein By the Lowry Method Using the ELx808 (2001-02-20)

Dual-Wavelength Measurements Compensate for Optical Interference (2004-05-07)

Kinetic Analysis of ß-Galactosidase Activity using the PowerWave™ HT and Gen5™ Data Analysis Software (2007-02-26)

Monitoring the Growth of E. coli With Light Scattering Using the Synergy™ 4 Multi-Mode Microplate Reader with Hybrid Technology™ (2008-01-15)

Nucleic Acid Purity Assessment Using A260/A280 Ratios (2001-02-06)

Phosphate and ATPase Determination Using the µQuant Scanning Microplate Spectrophotometer (2001-02-06)

PowerWave 200 Microplate Spectrophotometer: Spectral Scanning Capabilities (2001-02-06)

The Importance of the 240 nm Absorbance Measurement (2001-02-07)

The Kinetic ELISA Advantage in Quantitative Assays: Demonstrated with BioTek's PowerWave™ HT Microplate Reader and KC4™ Software (2006-03-27)

Drug Discovery – HTS

AlphaLISA Quantitation of Human Insulin Using a Hybrid Multi-Mode Microplate Reader, Synergy 4 (2007-12-10)

AlphaScreen™ Quantitation of cAMP using the Synergy™ 2 Multi-Mode Microplate Reader (2007-12-10)

An Introduction to Fluorescence Resonance Energy Transfer (FRET) Technology and its Application in Bioscience (2005-06-20)

Automation of the IDEXX Avian Influenza Virus Antibody Test Kit Using Individual Components and a Twister II from BioTek (2008-03-04)

Fluorescence Polarization Detection of Brucella abortus and Mycobacterium bovis specific antibodies Using Kits from Diachemix (2008-05-16)

FRET-Based Ion Channel, GPCR, and Kinase Assay Applications on the Synergy™ HT Multi-Detection Microplate Reader from BioTek (2005-03-01)

GeneBLAzer® FRET Cell-based Assay from Invitrogen™ on Synergy™ 4 (2007-12-20)

Gyrasol Kinase Assays (2008-09-26)

Invitrogen's Predictor™ hERG Fluorescence Polarization Assay Using BioTek's Synergy™ 4 Hybrid Microplate Reader (2008-07-21)

Invitrogen’s Adapta™ Universal Kinase Assay using BioTek’s Synergy™ 4 Hybrid Microplate Reader (2008-05-20)

Ion Channel Assay Development on the Synergy™ HT Multi Detection Microplate Reader from BioTek (2004-01-29)

LanthaScreen™ TR-FRET Assay from Invitrogen on Synergy 4 (2008-10-10)

Omnia Kinase Assay from Invitrogen on Synergy 4 (2007-12-19)

Optimizing Performance of the Transcreener® ADP Assay for the BioTek Synergy 2 and Synergy 4 Multi-Mode Microplate Readers (2008-09-17)

Oris - A Novel High Throughput-Compatible Cell Migration Screening Assay (2008-08-21)

Quantitate cAMP and Tumor Necrosis Factor Utilizing the Synergy™ 2 Multi-Detection Microplate Reader and HTRF® Assays for High-Throughput Screening (2007-03-26)

Z’-LYTE® Kinase Assay from Invitrogen™ on Synergy™ 4 (2007-11-15)

Fluorescence Detection

Detection of Calcium Concentration Changes Using the FLx800 Fluorescence Microplate Reader (2003-06-06)

Determination of Fluorescence Excitation and Emission Peaks for Qdot® Nanocrystals Using the Synergy™ 4 Multi-Mode Microplate Reader (2007-06-28)

Determination of Horseradish Peroxidase (HRP) Using Amplex Red and the Synergy HT Microplate Reader (2003-04-21)

Determination of NADH Concentrations with the Synergy™ 2 Multi-Detection Microplate Reader using Fluorescence or Absorbance (2006-12-04)

Determination of Relative Potency Using Parallel Line Analysis with Gen5 Data Analysis Software (2008-09-26)

Determination of ß-Galactosidase Activity using a BioTek Microplate Fluorescence Reader (2001-02-20)

Fluorescence-based Total Protein Determination using the Compound CBQCA (2001-02-20)

Fluorimetric Quantitation of Protein using the Reactive Compound Fluorescamine (2001-02-20)

Fluorometric Quantitation of dsDNA Using PicoGreen® (2006-12-04)

Fluorometric Quantitation of Hydrolyzed DNA (2001-02-20)

Fluorometric Quantitation of Protein using NanoOrange (2001-02-21)

Increasing the Range of DNA Quantitation when using Hoechst Dye 33258 (2001-02-09)

Measurement of Caspase Activity Using the Synergy HT Multi-Detection Microplate Reader: Measuring Apoptosis Enzymes (2004-07-28)

Performing Oxygen Radical Absorbance Capacity (ORAC) Assays with Synergy™ HT Multi-Detection Microplate Reader (2005-08-17)

Quantitation of Cholesterol Using a Fluorometric Assay and the Synergy HT Multi-Detection Microplate Reader (2005-02-28)

Quantitation of DNA using Hoechst 33258 (2001-02-21)

Quantitation of Green Fluorescent Protein in Microplates (2001-02-20)

Quantitation of Hydrogen Peroxide Using the Synergy HT (2002-08-20)

Quantitation of Peptides and Amino Acids with a Synergy HT using UV Fluorescence (2003-04-18)

Quantitation of RNA with RiboGreen Stain Using the FL600 Fluorescence Microplate Reader (2003-09-05)

Quantitation of ssDNA using OliGreen Fluorescent Stain (2001-02-21)

Quantitation of Total Protein Using OPA (2001-02-20)

Using BioTek's Synergy HT Reader to Measure Reactive Oxygen Species (ROS) Generation in Stimulated Cells (2008-11-05)

Using the Synergy HT to Measure Time-Resolved Fluorescent Compounds (2002-10-29)

General

Necessity is the Mother of Invention (2005-07-18)

Nucleic Acid & Protein Quantitation in the Microplate Format (2001-02-21)

Liquid Handling

Aliquoting Agar Containing Solutions with the MicroFlo™ Select Dispenser (2008-04-28)

Automating the Wash Steps of ELISPOT Assays using BioTek’s ELx405™ Microplate Washer (2006-09-19)

Automation of Routine Microplate Pipetting and Dispensing (2001-09-24)

Using the ELx50™ Filter Microplate Washer to Perform the Wash Processing Steps Required for Luminex xMAP Assays (2007-04-16)

Luminescence Detection

Detection of Mycoplasma Using the Synergy™ 2 Multi-Mode Microplate Reader and the Lonza MycoAlert Assay (2006-12-08)

Luciferase Measurements Using the Clarity™ Luminescence Microplate Reader (2004-06-09)

Luminescent Determination of ATP Concentrations Using the Clarity™ Luminescence Microplate Reader (2004-06-22)

Optimizing Dual-Glo™ Luciferase Assays with the Synergy™ HT Multi-Detection Microplate Reader (2005-02-23)

Quantitation of Human TNF-a with a QuantiGlo Assay from R&D Systems Using BioTek’s Synergy™ 2 Multi-Mode Microplate Reader (2008-05-21)

Using a BioTek Fluorescence Microplate Reader for Chemiluminescence Detection (2001-02-21)

Using the Clarity™ Luminescence Microplate Reader to Perform Dual-Luciferase® Assays (2004-06-09)

Using the Synergy™ HT Multi-Detection Microplate Reader to Run the Dual-Luciferase® Reporter Assay System (2005-02-15)