AlphaScreen - High Throughput Screening Assay Technology
AlphaScreen, used mostly in high throughput screening, is a homogenous assay technology similar to TR-FRET, to some extent, which allows detection of molecular events such as binding. Coated "Donor" and "Acceptor" beads are the basis of the AlphaScreen assay technology. AlphaScreen, a bead based assay, works through the interaction of the beads coming close to each other, resulting in a cascade of chemical reactions that act to produce a greatly amplified signal.
Traditionally an expensive process, BioTek has created unique, lower-cost options for AlphaScreen detection. The Synergy™ 2 and Synergy 4 Microplate Readers offer high-performance results without the use of a laser light source - reducing the cost of AlphaScreen detection while still providing results similar to more expensive systems.
If you are looking for a lower-cost solution for AlphaScreen detection, the Synergy 2 and Synergy 4 Plate Readers may be exactly what you are looking for. Synergy 2 is a five-mode microplate reader while Synergy 4 with patent-pending Hybrid Technology is the first in a new class of Hybrid Microplate Readers that combines the exquisite sensitivity of filter-based detection with the flexibility of monochromator-based detection, all in one compact unit.
Lower-Cost AlphaScreen Detection
Because AlphaScreen detection has traditionally been performed with the help of a dedicated laser excitation source, AlphaScreen-compatible readers have been very expensive. Now, with Synergy 2 and Synergy 4 from BioTek, AlphaScreen detection has a lower-cost solution. Synergy 2 and Synergy 4 both offer AlphaScreen detection with performance proven similar to more expensive laser-based systems. In addition to AlphaScreen, the Synergy 2 and Synergy 4 plate readers have also been shown to perform extremely well with other major screening assay technologies.
Learn more about these readers and their AlphaScreen detection and other screening assay capabilities:
AlphaScreen technology is only one of the many screening assay technologies. In addition to AlphaScreen, other popular homogeneous assay technologies include TR-FRET, FRET, Fluorescence Polarization and Luminescence. Like AlphaScreen, all of these technologies can be easily automated and miniaturized. Synergy 2 and Synergy 4 have both been shown, through partnerships and field tests, to perform extremely well not only for AlphaScreen, but also for all these detection technologies. It is this price / performance ratio coupled with their unique ability to deliver high-performance AlphaScreen results without using an expensive laser light source that sets Synergy 2 and Synergy 4 apart.
For AlphaScreen and other popular homogeneous assay technologies, Synergy 2 and Synergy 4 offer the superior performance you expect from BioTek Instruments, in a lower-cost solution.
To learn more about the Synergy 2 and Synergy 4, their AlphaScreen capabilities, and the other detection technologies they work with, visit our Microplate Reader Home Page.
Detection Data Analysis Software
The industry-leading Gen5™ Data Analysis Software controls Synergy 2 and Synergy 4 Multi-Mode Microplate Readers. Utilizing its unique reader control functions, Gen5 can be quickly programmed to run a variety of assays, including high-throughput AlphaScreen assays. By providing efficient workflow and a logical interface, this software enables quick and easy transition between reading parameters, plate layout, and data reduction - creating customized reports in a publication-ready form.
From AlphaScreen to high throughput screening, we invite you to learn more about how we leverage leading microplate technology to achieve optimal results through products such as Synergy 2, Synergy 4 and more.